Improvement of the QuEChERS extraction step by matrix-dispersion effect and application on beta-lactams analysis in wastewater sludge by LC-MS/MS

Guironnet, Alexandre; Wiest, Laure; Vulliet, Emmanuelle

doi:10.1016/j.talanta.2021.122923

Cited by 12 publications

(3 citation statements)

References 37 publications

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“…The sample collection and preparation were described in detail by Guironnet et al [33]. Briefly, 500 mg of freeze-dried sludge was mixed with 2 g of EDTA-treated sand before dispersive-QuEChERS extraction was performed using the citrate buffer with 5 mL of 0.1 M EDTA and 10 mL of ACN.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

“…Firstly, each compound was infused at 100 ng.mL -1 in 50:50 water: ACN. As MRM 3 is the fragmentation of a 1 st generation fragment ion, the first step consists in the development of the MRM parameters, which was described in Guironnet et al [33]. In case of CEF, the selected MRM transition was m/z=524 (P) to m/z=241 (F).…”

Section: Optimisation Of Mrm 3 Parametersmentioning

confidence: 99%

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Advantages of MS/MS/MS (MRM3) vs classic MRM quantification for complex environmental matrices: Analysis of beta-lactams in WWTP sludge

Guironnet

Wiest

Vulliet

2022

Analytica Chimica Acta

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Section: Sample Collection and Preparationmentioning

confidence: 99%

Section: Optimisation Of Mrm 3 Parametersmentioning

confidence: 99%

Advantages of MS/MS/MS (MRM3) vs classic MRM quantification for complex environmental matrices: Analysis of beta-lactams in WWTP sludge

Guironnet

Wiest

Vulliet

2022

Analytica Chimica Acta

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“…However, these assays are highly time-consuming and expensive, have a high percentage of false positives and, in many cases, quantitative determination is not possible. Currently, analytical investigation of cloxacillin in biological fluids, environmental samples and food requires the use of high-performance liquid chromatography (HPLC) combined with ultraviolet (UV) [11], diode array detector (DAD) [12,13], fluorescence detection (FLD) [14], mass spectrometry (MS) [15,16] or tandem mass liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) [17][18][19][20], which is considered the preferred detection method for routine analysis in a complex matrix because of its great sensitivity and selectivity. Although these methods are rather efficient for the determination of cloxacillin in different matrices, some of them have disadvantages such as low sensitivity, time-consuming sample pretreatment, high-cost instrumentation and difficulties in sample preparation [16,17,19].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Molecularly Imprinted Polymers as Selective Solid-Phase Extraction Sorbents for the Detection of Cloxacillin in Drinking and River Water

Garcinuño,

Collado,

Paniagua

et al. 2023

Polymers

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This paper describes a new methodology for carrying out quantitative extraction of cloxacillin from drinking and river water samples using a molecularly imprinted polymer (MIP) as a selective sorbent for solid-phase extraction (MISPE). Several polymers were synthesized via thermal polymerization using cloxacillin as a template, methacrylic acid (MAA) as a functional monomer, ethyleneglycoldimethacrylate (EGDMA) as a cross-linker and different solvents as porogens. Binding characteristics of the adequate molecularly imprinted and non-imprinted (NIP) polymers were evaluated via batch adsorption assays following the Langmuir and Freundlich isotherms and Scatchard assays. The parameters related to the extraction approach were studied to select the most appropriate polymer for cloxacillin determination. Using the optimized MIP as the SPE sorbent, a simple sample treatment methodology was combined with high-performance liquid chromatography (HPLC) to analyze cloxacillin residues in drinking and river water. Under the optimum experimental conditions, the MISPE methodology was validated using spiked samples. The linearity for cloxacillin was assessed within the limits of 0.05–1.5 µg L−1 and the recovery percentage was higher than 98% (RSD < 4%). The limits of detection and limits of quantification were 0.29 and 0.37 µg L−1 and 0.8 and 0.98 µg L−1 for drinking and river water, respectively. The selectivity of MIP against other ß-lactam antibiotics with similar structures (oxacillin, cefazoline, amoxicillin and penicillin V) was studied, obtaining a good recovery higher than 85% for all except cefazoline. The proposed MISPE-HPLC methodology was successfully applied for the detection of cloxacillin in drinking water from Canal de Isabel II (Madrid) and river water from the Manzanares River (Madrid).

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Quantification of 16 cephalosporins in the aquatic environment by liquid chromatography‐tandem mass spectrometry

Kaddah

Al-Dokhmaisy

Mansour

et al. 2022

J of Separation Science

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Antimicrobial agents are essential to protect human and animal health. During the coronavirus disease 2019 pandemic, antimicrobials such as cephalosporins were widely used as prophylactics and to prevent bacterial co-infection.Undoubtedly, the prevalence of antibiotics in the aquatic environment will ultimately affect the degree of resistance against these bacteria in animals and the environmental systems. In order to monitor 16 cephalosporins in the aquatic environment, we developed a new liquid chromatography-tandem mass spectrometry method that functioned simultaneously under positive and negative electrospray ionization switching modes. The chromatographic separation has been implemented using a pentafluorophenyl propyl column kept at 40 • C. The limits of detection and quantitation for the studied cephalosporins ranged from (8 × 10 −4 ) to (7.11 × 10 −2 ) ng/ml and from (2.61 × 10 −3 ) to (2.37 × 10 −1 ) ng/ml, respectively. The percent extraction efficiency (apparent recovery) and relative standard deviations for the analyzed cephalosporins ranged from 61.69% to 167.67% and 2.45% to 13.48%, respectively. The overall findings showed that the effluent from the wastewater treatment plants that receive wastewater from pharmaceutical factories had a higher detected amount of cephalosporins than that of domestic sewage. Moreover, seven cephalosporins, including cefuroxime, ceftazidime, cefradine, cefprozil, cefixime, cefalexin, and cefadroxil (0.68-105.45 ng/L) were determined in the aquatic environment.

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Improvement of the QuEChERS extraction step by matrix-dispersion effect and application on beta-lactams analysis in wastewater sludge by LC-MS/MS

Cited by 12 publications

References 37 publications

Advantages of MS/MS/MS (MRM3) vs classic MRM quantification for complex environmental matrices: Analysis of beta-lactams in WWTP sludge

Advantages of MS/MS/MS (MRM3) vs classic MRM quantification for complex environmental matrices: Analysis of beta-lactams in WWTP sludge

Assessment of Molecularly Imprinted Polymers as Selective Solid-Phase Extraction Sorbents for the Detection of Cloxacillin in Drinking and River Water

Quantification of 16 cephalosporins in the aquatic environment by liquid chromatography‐tandem mass spectrometry

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