Improvement of the technique for the bovine genome editing using the example of knockout of the CD 209 receptor responsible to the bovine leukemia virus

Abstract

The aim of this work was to evaluate the possibility of editing the CD209 gene associated with bovine leukemia virus (BLV) infection. Two ways of delivering the gene editing system into the zygote were investigated: delivery of the CRISPR/Cas9 system using a viral vector and injection of mRNA and sgRNA into the oocyte cytoplasm. The most effective was the microinjection of spCas9 protein mRNA and sgRNA targeting CD209 at the zygote stage into the cytoplasm.