Improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles

Mukhopadhyay, Arka; Dasgupta, Anjan Kumar; Chattopadhyay, Dhrubajyoti; Chakrabarti, Krishanu

doi:10.1016/j.biortech.2012.03.094

Cited by 36 publications

(38 citation statements)

References 31 publications

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“…The adsorption/desorption characteristics [44,220,[228][229][230] and conformation changes [230][231][232][233][234][235] of various kinds of proteins (or peptides) on HAp surfaces have been studied. Mukhopadhyay et al [236] recently reported that HAp nanoparticle supplementation increases thermal stability of pectate lyase from Bacillus megaterium, that is, this enzyme can retain high activity at elevated temperatures (up to 90…”

Section: Carriers For Drug Protein and Gene Deliverymentioning

confidence: 99%

Hydroxylapatite nanoparticles: fabrication methods and medical applications

Okada

Furuzono

2012

Science and Technology of Advanced Materials

126

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Hydroxylapatite (or hydroxyapatite, HAp) exhibits excellent biocompatibility with various kinds of cells and tissues, making it an ideal candidate for tissue engineering, orthopedic and dental applications. Nanosized materials offer improved performances compared with conventional materials due to their large surface-to-volume ratios. This review summarizes existing knowledge and recent progress in fabrication methods of nanosized (or nanostructured) HAp particles, as well as their recent applications in medical and dental fields. In section 1, we provide a brief overview of HAp and nanoparticles. In section 2, fabrication methods of HAp nanoparticles are described based on the particle formation mechanisms. Recent applications of HAp nanoparticles are summarized in section 3. The future perspectives in this active research area are given in section 4.

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Section: Carriers For Drug Protein and Gene Deliverymentioning

confidence: 99%

Hydroxylapatite nanoparticles: fabrication methods and medical applications

Okada

Furuzono

2012

Science and Technology of Advanced Materials

126

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“…The stimulatory effect of hydroxyapatite nanoparticles on pectate lyase up to 10.5 lg mL -1 due to chaperon-like activity has also been reported by Dutta et al [22]. Additionally, the elevation of native state activity of the enzyme may be responsible for the observed increase as reported for pectate lyase in the presence of hydroxyapatite nanoparticles [23]. Higher stability of keratinase exposed to iron oxide nanoparticles is also known [24].…”

Section: Response To Bulk and Nano-zno In Legumesmentioning

confidence: 59%

Effect of nano-zinc oxide on nitrogenase activity in legumes: an interplay of concentration and exposure time

2015

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Experiments were carried out to study the effect of zinc oxide nanoparticles (nano-ZnO) on nitrogenase activity in legumes. In the first experiment, nodulated roots of cluster bean, moth bean, green gram and cowpea were dipped in Hoagland solution containing 1.5 and 10 lg mL -1 of nano-ZnO for 24 h. Nitrogenase activity in cluster bean, green gram and cowpea roots increased after dipping in solution containing 1.5 lg mL -1 nano-ZnO, but decreased in roots dipped in solution containing 10 lg mL -1 nano-ZnO. However, in moth bean roots, nitrogenase activity decreased after dipping in solution containing either concentration of nano-ZnO. In the second experiment, nodulated roots of green gram were dipped in Hoagland solution containing 1, 4, 6, 8 and 10 lg mL -1 nano-ZnO for 6-30 h before estimating nitrogenase activity. Results showed that an interactive effect of nanoZnO concentration and exposure time influenced nitrogenase activity. The possible reasons behind this effect have been discussed. A model [A = 3.44 ? 0.46t -0.01t 2 -0.002tc 2 (R 2 = 0.81)] involving linear and power components was developed to simulate the response of nitrogenase activity in green gram roots to the concentration and exposure time of nano-ZnO.

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“…In recent work reported from our laboratory, the addition of cofactors (metal) in nano form greatly enhanced the activity and thermal stability of pectate lyase and laccase (Mukhopadhyay et al, 2012(Mukhopadhyay et al, , 2013a. Such addition also restored the functional properties of a functionally impaired pectate lyase (Dutta et al, 2014).…”

Section: Introductionmentioning

confidence: 92%

“…Functionalized carbon nanotubes have been studied for many potential applications, including immobilization of proteins, to improve the physical stability (Gupta et al, 2002;Cang and Pastorin, 2009). Nano-materials exhibit properties such as large surface-to-volume ratios, high surface reaction activity, high catalytic efficiency, and strong adsorption ability (Mukhopadhyay et al, 2012). Enzyme adsorption on nanoparticles resulted in improved activity (Lynch and Dawson, 2008).…”

Section: Introductionmentioning

confidence: 99%