Improvement of tissue culture, genetic transformation, and applications of biotechnology toBrassica

Abstract

Development of in vitro plant regeneration method from Brassica explants via organogenesis and somatic embryogenesis is influenced by many factors such as culture environment, culture medium composition, explant sources, and genotypes which are reviewed in this study. An efficient in vitro regeneration system to allow genetic transformation of Brassica is a crucial tool for improving its economical value. Methods to optimize transformation protocols for the efficient introduction of desirable traits, and a comparative analysis of these methods are also reviewed. Hence, binary vectors, selectable marker genes, minimum inhibitory concentration of selection agents, reporter marker genes, preculture media, Agrobacterium concentration and regeneration ability of putative transformants for improvement of Agrobacterium-mediated transformation of Brassica are discussed.

The Impact of Heat Stress on Morpho-Physiological Response and Expression of Specific Genes in the Heat Stress-Responsive Transcriptional Regulatory Network in Brassica oleracea

“…Many commonly cultivated Brassica vegetables such as cabbage, broccoli, cauliflower, kale and brussels sprouts belong to the Brassica oleracea species [1]. They are low-calorie and serve as a rich source of glucosinolates and carotenoids, as well as other vitamins, minerals and anthocyanins that are beneficial to human health [2,3]. However, Brassica vegetables are cool season crops, and most varieties are highly vulnerable to high temperature which stimulates various and often incompatible changes in plant growth, development and physiological processes which together adversely affect crop yield [4].…”

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

“…Usually, the selectable marker gene is positioned in the DNA vector construct along with the desired gene. In plant transformation, dominant selectable marker genes are developed based on genes that confer resistance to antibiotics or herbicides or have the ability to digest non-metabolizable substances (Miki & McHugh, 2004;Ravanfar et al, 2017). Resistance genes for antibiotics (hygromycin and kanamycin) and herbicides (bialaphos, glyphosate and the bar) have been used extensively as selection tools (Pandeya et al, 2018).…”

The Brassica napus L. plants expressing antimicrobial peptide cecropin P1 are safe for colonization by beneficial associative microorganisms

“…Oilseed rape (Brassica napus L.) is an important oilseed and fodder crop (Peng et al 2018;Hegelian et al 2018), producing valuable biotechnological products (Ravanfar et al 2017). Many transgenic herbicide-resistant Brassica napus plants (Nishizawa et al 2016;Zhang et al 2018), and plants resistant to insects (Wang et al 2005), nematodes (Zhong et al 2019), and certain diseases (Larkan et al 2013;Ziaei et al 2016;Aghazadeh et al 2016) are also known.…”