We studied the proteolysis and conducted a sensory evaluation of fermented
sausages using strains derived from
Kimchi
[
Pediococcus
pentosaceus
-SMFM2021-GK1 (GK1);
P.
pentosaceus
-SMFM2021-NK3 (NK3)],
Doenjang
[
Debaryomyces hansenii-
SMFM2021-D1 (D1)], and spontaneous
fermented sausage [
Penicillium nalgiovense-
SMFM2021-S6 (S6)].
Fermented sausages were classified as commercial starter culture (CST), mixed
with GK1, D1, and S6 (GKDS), and mixed with NK3, D1, and S6 (NKDS). The protein
content and pH of GKDS and NKDS were significantly higher than those of CST on
days 3 and 31, respectively (p<0.05). Sodium dodecyl
sulfate–polyacrylamide gel electrophoresis showed that the NKDS had
higher molecular weight proteins than the GKDS and CST. The myofibrillar protein
solubility of the GKDS and NKDS was significantly higher than that of the CST on
day 31 (p<0.05). The GKDS displayed significantly higher pepsin and
trypsin digestion than the NKDS on day 31 (p<0.05). The hardness,
chewiness, gumminess, and cohesiveness of the GKDS were not significantly
different from those of the CST. The GKDS exhibited the highest values for
flavor, tenderness, texture, and overall acceptability. According to this study,
sausages fermented using lactic acid bacteria (GK1), yeast (D1), and mold (S6)
derived from Korean fermented foods displayed high proteolysis and excellent
sensory evaluation results.