Improvements in algal lipid production: a systems biology and gene editing approach

Banerjee, Avik; Banerjee, Chiranjib; Negi, Sangeeta; Chang, Jo Shu; Shukla, Pratyoosh

doi:10.1080/07388551.2017.1356803

Cited by 72 publications

(28 citation statements)

References 120 publications

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“…Several genera of microalgae have been used for biofuel production, and metabolic models now exist for organisms such as Chlamydomonas [19–30], Chlorella [31–35], Nannochloropsis [36–38], Synechocystis [39–46], Tetraselmis [47], Monoraphidium [48], Ostreococcus [49], Tisochrysis [50], and Phaeodactylum [51–54]. The genetic tractability of several microalgae ( Chlamydomonas , Synechocystis , Phaeodactylum ) [55] also renders them interesting for gene-knockout studies using metabolic modeling tools. Metabolic models have enabled retrieving key information about central carbon metabolism, nutrient dependence, and distribution of reactions throughout different compartments in these organisms.…”

Section: Introductionmentioning

confidence: 99%

Advances in metabolic modeling of oleaginous microalgae

Tibocha‐Bonilla

Zuñiga

Godoy-Silva

et al. 2018

Biotechnol Biofuels

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Production of biofuels and bioenergy precursors by phototrophic microorganisms, such as microalgae and cyanobacteria, is a promising alternative to conventional fuels obtained from non-renewable resources. Several species of microalgae have been investigated as potential candidates for the production of biofuels, for the most part due to their exceptional metabolic capability to accumulate large quantities of lipids. Constraint-based modeling, a systems biology approach that accurately predicts the metabolic phenotype of phototrophs, has been deployed to identify suitable culture conditions as well as to explore genetic enhancement strategies for bioproduction. Core metabolic models were employed to gain insight into the central carbon metabolism in photosynthetic microorganisms. More recently, comprehensive genome-scale models, including organelle-specific information at high resolution, have been developed to gain new insight into the metabolism of phototrophic cell factories. Here, we review the current state of the art of constraint-based modeling and computational method development and discuss how advanced models led to increased prediction accuracy and thus improved lipid production in microalgae.

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Section: Introductionmentioning

confidence: 99%

Advances in metabolic modeling of oleaginous microalgae

Tibocha‐Bonilla

Zuñiga

Godoy-Silva

et al. 2018

Biotechnol Biofuels

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“…Additionally, in the cells of Haematococcus pluvialis , blue light promoted the accumulation of astaxanthin, a value‐added antioxidant for human diet . (v) Genetic modification technology is widely applied to enhance the synthesis of certain compositions, particularly protein and lipid, in microalgae . Molecular genetic tools that have been used for genetic modification of algae include transformation methods, mutagenesis and breeding, and modulation of the endogenous genome using CRISPR systems .…”

Section: Microalgae Biotechnology For Blssmentioning

confidence: 99%

Microalgae biotechnology as an attempt for bioregenerative life support systems: problems and prospects

Yang

Liu

et al. 2019

J of Chemical Tech & Biotech

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Bioregenerative life support system (BLSS) plays a key role in the long‐duration manned space mission. Microalgae‐based BLSS was proposed decades ago but it had a variety of technical problems hindering its application in space mission. However, in recent years, a lot of effort has been devoted to this field and great progresses have been achieved in the research of microalgae‐based BLSS. Therefore, the main aim of this work is to summarize the previous progress and find the challenges faced by microalgae‐based BLSS technology. In this regard, this works introduces the concept of microalgae‐based BLSS and presents its development paths in the past decades. Additionally, technology updates in the aspects of microalgae‐based oxygen generation, value‐added biomass production as food alternative, and wastes treatment by microalgae are summarized. Challenges faced by microalgae‐based BLSS and potential solutions are discussed. This work provides a deep understanding of microalgae‐based BLSS and that it would be beneficial for promoting implementation of this novel technology in manned space missions. © 2019 Society of Chemical Industry

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“…One alternative to "classic" approaches targeting single enzymes is to identify master regulators of FA metabolism whose modification is instead expected to provide a more global remodeling of their accumulation. In such context, transcription factors (TF) play a critical role and are indeed viable targets for lipids biosynthesis biotechnological optimization (Hu et al 2014;Banerjee et al 2018).…”

Section: Improving the Content Of Bio-active Compoundsmentioning

confidence: 99%

“…The above discussed possible drawbacks of LHC reduction might however be counterbalanced by more targeted approaches, in which only LHC involved in light harvested will be affected, also potentially balancing their reduction among the two PS. The development of CRISPR-based genome editing approches is indeed opening such possibility, by targeting also microlgae species with a larger industrial potential (Banerjee et al 2018;Verruto et al 2018).…”

Section: Metabolic Engineering To Increase Microalgae Light-use Efficmentioning

confidence: 99%

“…leaving room for implementing such technology also in microalgae. In the future, identifying novel candidates(Sugiyama et al 2017;Lao et al 2018) and engineering simoultaneously multiple enzymatic steps in the pathway might strengthen the metabolic flux redirection towards carotenoids synthesis with no secondary effects on cell physiology and the development of effective targeted genome editing approaches is currently opening such possibility(Nymark et al 2016;Banerjee et al 2018).…”

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confidence: 99%

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Potential of Microalgae Biomass for the Sustainable Production of Bio-commodities

Perin

Morosinotto

2019

Progress in Botany

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Human activities are causing major negative environmental impacts and the development of sustainable processes for production of commodities is a major urgency. Plants biomass represents a valuable alternative to produce energy and materials but exploiting present crops for commodities production would however require massive resources (i.e. land, water and nutrients), raising serious sustainability concerns. In addition to efforts to improve plants land and resources use efficiency, it is thus fundamental to look for alternative sources of biomass to complement crops. Microalgae are unicellular photosynthetic organisms that show a huge, yet untapped, potential in this context. Microalgae metabolism is powered by photosynthesis and thus uses sunlight, a renewable energy source and the exploitation of microalgae-based products has the potential to provide a beneficial environmental impact. These microorganisms have the ability to synthesise a wide spectrum of bioactive compounds, with many different potential applications (e.g. nutraceutics/pharmaceutics and biofuels). Several, still unresolved, challenges are however present such as the lack of cost-effective cultivation platforms and biomass-harvesting technologies. Moreover, the natural metabolic plasticity of microalgae is not optimized for a production at scale and low biomass productivity and product yields affect competitiveness. Tuning microalgae metabolism to maximize productivity thus represents an unavoidable challenge to reach the theoretical potential of such organisms.

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Improvements in algal lipid production: a systems biology and gene editing approach

Cited by 72 publications

References 120 publications

Advances in metabolic modeling of oleaginous microalgae

Advances in metabolic modeling of oleaginous microalgae

Microalgae biotechnology as an attempt for bioregenerative life support systems: problems and prospects

Potential of Microalgae Biomass for the Sustainable Production of Bio-commodities

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