Improvements in the intraluminal thread technique to induce focal cerebral ischaemia in rabbits

Kong, Ling Qi; Xie, Jing Xia; Han, Hongbin; Liu, Hong Dong

doi:10.1016/j.jneumeth.2004.03.017

Cited by 9 publications

(4 citation statements)

References 14 publications

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“…Although this method has many variants, particularly with respect to the construction of the occluding thread and closure of additional vessels to manipulate collateral blood flow, the basic technique described originally by Koizumi et al (1986) and modified by Longa et al (1989) involves introducing an occluding thread into the extracranial internal carotid artery (ICA) and advancing it until its tip occludes the origin of the MCA. Although most frequently applied to rats and mice, the method has also been used in rabbits (Kong et al , 2004), gerbils, (Baskaya et al , 1999) and marmosets (Freret et al , 2008). In baboons, the concept has been extended to the use of a balloon catheter or wire coil introduced through either the carotid (Gao et al , 2006) or femoral arteries (Hamberg et al , 2002) to occlude the MCA.…”

Section: Different Approaches To Induction Of Focal Ischemiamentioning

confidence: 99%

Different Strokes for Different Folks: The Rich Diversity of Animal Models of Focal Cerebral Ischemia

Howells

Porritt

Rewell

et al. 2010

J Cereb Blood Flow Metab

272

231

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No single animal model is able to encompass all of the variables known to affect human ischemic stroke. This review highlights the major strengths and weaknesses of the most commonly used animal models of acute ischemic stroke in the context of matching model and experimental aim. Particular emphasis is placed on the relationships between outcome and underlying vascular variability, physiologic control, and use of models of comorbidity. The aim is to provide, for novice and expert alike, an overview of the key controllable determinants of experimental stroke outcome to help ensure the most effective application of animal models to translational research.

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Section: Different Approaches To Induction Of Focal Ischemiamentioning

confidence: 99%

Different Strokes for Different Folks: The Rich Diversity of Animal Models of Focal Cerebral Ischemia

Howells

Porritt

Rewell

et al. 2010

J Cereb Blood Flow Metab

272

231

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show abstract

“…The major advantage is the ability to precisely control the timing of reperfusion using the thread occlusion model plus produce discrete contiguous infarcts. In comparison, embolic models often produce multiple emboli and resultant patchy infarcts due to clot fragmentation; the endothelin-1-induced MCAo model is limited by the lack of control over the timing of reperfusion, while the rabbit intraluminal MCAo model is difficult to perform due to the variations in the anatomy of the internal carotid artery (15), despite improvements in the stability and reproducibility of the model (16). Furthermore, we have demonstrated that humane survival of animals for 24 h poststroke is possible, and that development of a system of coregistration of histology allows pixel-based analysis using a complete or a near-complete histological outcome.…”

Section: Discussionmentioning

confidence: 99%

Establishing a Rodent Stroke Perfusion Computed Tomography Model

McLeod

Parsons

Levi

et al. 2011

International Journal of Stroke

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Brain computed tomography perfusion imaging in acute stroke may help guide therapy. However, the perfusion thresholds defining potentially salvageable (penumbra) and irreversibly injured (infarct core) tissue require further validation. The aim of this study was to validate infarct core and penumbra perfusion thresholds in a rodent stroke model by developing and optimising perfusion computed tomography imaging, performing serial scanning and correlating scans with final histology. Stroke was induced in male Wistar rats (n=17) using the middle cerebral artery thread-occlusion method. Perfusion computed tomography scans were obtained immediately pre- and postocclusion, and every 30 min for 2.5 h. Histological changes of infarction were assessed after 24 h. High-quality maps of cerebral blood flow and cerebral blood volume were generated at multiple coronal planes after optimisation of contrast injection and scanning parameters. The prestroke absolute cerebral blood flow and cerebral blood volume values (mean ± SD) were 158.2 ± 49.94 ml/min per 100 g and 5.6 ± 1.13 ml per 100 g, respectively. Cerebral blood flow was significantly lower in the infarct region of interest than the contralateral hemisphere region of interest at all time points, except the 0.5 h postocclusion time point. However, cerebral blood volume was only significantly lower in the infarct region of interest than the contralateral hemisphere region of interest at the 1 h and the 1.5 h time points (postocclusion). This study has demonstrated for the first time the feasibility of performing perfusion computed tomography in the most commonly used animal model of stroke. The model will allow definitive studies to determine optimal thresholds and the reliability of perfusion computed tomography measures for infarct core and penumbra.

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“…Наилучшие результаты были получены при одновременном введении двух окклюдирующих нитей с диаметром кончика 0,51-0,55 мм на глубину 50-60 мм по ходу внутренней сонной артерии. Поскольку наиболее частым нежелательным явлением при проведении эндоваскулярной окклюзии СМА является интракраниальное кровоизлияние, авторы рекомендуют использовать нити диаметром не менее 0,50 мм и осуществлять их введение максимально аккуратно, избегая возможного нарушения целостности артерий [43].…”

Section: эндоваскулярная окклюзия смаunclassified

Rabbit models of ischemic stroke in biomedical research

Sysoev¹,

Prikhodko²,

Ивкин³

et al. 2019

Pharmacy Formulas

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The development of experimental models of ischemic stroke allowing for effective translation of results from animal studies to humans is an important task of modern neuropharmacology. Due to the fact that new drugs with neuroprotective properties that have shown activity in rodents most often turn out to be not effective enough in humans, the use of larger laboratory animals may become a viable solution. Among the latter, rabbits are the most accessible species for laboratories. Since they have gyrencephalic brains and a significant amount of white matter, they can be considered to be highly similar to humans. In the present review, we discussed the main methods of ischemic stroke modelling in rabbits. The breed, age and sex of the animals, anesthesia methods as well as criteria for functional state assessment after cerebral ischemia are the issues that should be addressed before the start of the study.

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Improvements in the intraluminal thread technique to induce focal cerebral ischaemia in rabbits

Cited by 9 publications

References 14 publications

Different Strokes for Different Folks: The Rich Diversity of Animal Models of Focal Cerebral Ischemia

Different Strokes for Different Folks: The Rich Diversity of Animal Models of Focal Cerebral Ischemia

Establishing a Rodent Stroke Perfusion Computed Tomography Model

Rabbit models of ischemic stroke in biomedical research

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