Improving anaerobic digestion of a cellulosic waste via routine bioaugmentation with cellulolytic microorganisms

Martin-Ryals, Ana; Schideman, Lance; Li, Peng; Wilkinson, H. T.; Wagner, Richard E.

doi:10.1016/j.biortech.2015.03.069

Cited by 95 publications

(38 citation statements)

References 16 publications

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“…In agreement with our results, Tsapekos and co-workers showed in a previous study [1] that bioaugmentation cultures did not significantly affect the microbial community composition of anaerobic digesters and the abundance of used strains did not increase after bioaugmentation. These results match those observed in an earlier study carried out by Martin-Ryals and colleagues, in which bioaugmentation cultures could not be sustained in semi-continuous reactors [5]. This effect might be due to competition with the seed microbiota and/or the lack of the ability to adapt to the given environment [20].…”

Section: Microbial Community Profilessupporting

confidence: 90%

Bioaugmentation of anaerobic digesters treating lignocellulosic feedstock by enriched microbial consortia

Özbayram

Kleinsteuber

Nikolausz

et al. 2018

Engineering in Life Sciences

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Three different bioaugmentation cultures enriched from natural and engineered cellulolytic environments (cow and goat rumen, a biogas reactor digesting sorghum biomass) were compared for their enhancement potential on the anaerobic digestion of wheat straw. Methane yields were determined in batch tests using the Automatic Methane Potential Test System operated for 30 days under mesophilic conditions. All cultures had positive effects on substrate degradation, and higher methane yields were observed in the bioaugmented reactors compared to control reactors set up with standard inoculum. However, the level of enhancement differed according to the type of the enrichment culture. Methane yield in batch reactors augmented with 2% cow rumen derived enrichment culture was increased by only 6%. In contrast, reactors amended with 2% goat rumen derived enrichment culture or with the bioaugmentation culture obtained from the biogas reactor digesting sorghum biomass produced 27 and 20% more methane, respectively. The highest methane yield was recorded in reactors amended with 6% goat rumen derived enrichment culture, which represented an increase by 36%. The microbial communities were quite similar at the end of the batch tests independently of the bioaugmentation sources, indicating that the introduced microbial communities of the enrichment cultures did not dominate the reactors.

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Section: Microbial Community Profilessupporting

confidence: 90%

Bioaugmentation of anaerobic digesters treating lignocellulosic feedstock by enriched microbial consortia

Özbayram

Kleinsteuber

Nikolausz

et al. 2018

Engineering in Life Sciences

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“…Thus, animal manure is one of the most widely used feedstock for anaerobic digesters. However, due to it is recalcitrant structure, hydrolysis is accepted as a rate-limiting step (Martin-Ryals et al 2015). Thus, new strategies should be proposed to improve hydrolysis of lignocellulose-rich feedstock to enhance the economic feasibility of biogas plants.…”

Section: Introductionmentioning

confidence: 99%

Rumen bacteria at work: bioaugmentation strategies to enhance biogas production from cow manure

et al. 2018

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“…To this end, there are approaches that would help to keep a productive and robust mixed culture. For example, bioaugmentation to enrich with the desired micro‐organisms, selection pressure to curtail the growth of unwanted micro‐organisms, or adaptive evolution to confer growth advantages to the desired micro‐organisms . With the help of synthetic biology, the desired micro‐organisms can be co‐cultured and programmed to perform ‐specific targets under the desired conditions (not ‐necessarily synthetic engineered species), which is a relatively unexplored research area in the field of second‐generation biofuels.…”

Section: Resultsmentioning

confidence: 99%

Proposal for biorefineries based on mixed cultures for lignocellulosic biofuel production: a techno‐economic analysis

Valdez‐Vazquez

Sánchez

2017

Biofuels Bioprod Bioref

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An innovative conceptual design is introduced for lignocellulosic-based biochemical platform biorefi neries using three mixed-culture bioprocesses that sequentially disintegrate each polysaccharide fraction into different target biofuels: hydrogen, methane, and fuel butanol. This mixed-culture biorefi nery circumvents the use of corrosive chemicals, energy-demanding pre-treatments, costly enzymes, separate units for saccharifi cation and fermentation, steam for sterilization, and expensive steel bioreactors. The concept mimics bioprocesses occurring in nature to degrade complex substrates. A technoeconomic analysis of these biorefi neries is carried out focusing on the impact of residence times (8-120 h) and butanol titers (10-20 g/L) of the production of acetone-butanol-ethanol (ABE) on the total production costs (TPC) of butanol. The design includes electricity-steam cogeneration from gaseous biofuels and lignin, as well as solvent purifi cation. Simulation results show that the highest butanol titer impacts TPC to a greater extent than the lowest residence time. TPC range from US$1.04 to US$1.27 per liter of butanol in facilities with 20 g/L of butanol irrespective of residence time. The end-use energy ratio for all facilities was close to 2 or higher. These biorefi neries display lower energy consumption and environmental impacts than conventional second-generation lignocellulosic biofuels biorefi neries, and its cost structure is determined by the substrate similar to bioprocesses with mature technology. Finally, this study provides an insight to the advancements made in realizing viable mixed-culture fermentations for different fi elds of biotechnology and therefore might be encouraging for further studies of lignocellulosic biorefi neries based on mixed cultures.

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Improving anaerobic digestion of a cellulosic waste via routine bioaugmentation with cellulolytic microorganisms

Cited by 95 publications

References 16 publications

Bioaugmentation of anaerobic digesters treating lignocellulosic feedstock by enriched microbial consortia

Bioaugmentation of anaerobic digesters treating lignocellulosic feedstock by enriched microbial consortia

Rumen bacteria at work: bioaugmentation strategies to enhance biogas production from cow manure

Proposal for biorefineries based on mixed cultures for lignocellulosic biofuel production: a techno‐economic analysis

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