Improving animal research reporting standards

Osborne, Nikki; Anestidou, Lida; Ritskes‐Hoitinga, Merel; Griffin, Gilly

doi:10.15252/embr.201846069

Cited by 41 publications

(26 citation statements)

References 11 publications

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“…However, modeling of enterovirus infections in animals has certain features that are primarily due to their selective sensitivity to particular types of enteroviruses. Thus, for Coxsackie B viruses effective models are laboratory mice of 1-3 days of age, among polioviruses only 2 serotypes cause clinical symptoms in adult mice, and only with parenteral administration, and to mouse ECOS viruses are not at all sensitive [2,7,11,12]. In general, it should be noted that despite the presence of a certain amount of scientific work on modeling of enterovirus infection in animals, detailed information on the features of the course and factors affecting the infection process is not enough [17].…”

Section: Discussionmentioning

confidence: 99%

Modeling of viral-bacterial infections against antibiotic-induced intestinal dysbiosis

Bobyr¹,

Stechenko²,

Shyrobokov³

et al. 2019

Rep. of Morph.

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The study of the role of viral-bacterial associations in the etiology of acute intestinal infections is considered new to medical microbiology. The purpose of the study was to determine the effect of viral-bacterial associations on the manifestation of virulence of pathogens and the degree of development of structural-morphological disorders of the internal organs in animals with antibiotic-induced dysbiosis. In a study of 210 white laboratory mice, BALB/c lines formed dysbiosis using antibacterial agents (ampicillin, gentamicin, metronidazole) followed by simulation of the experimental infection. To simulate salmonella infection, mice were infected with a clinical strain of Salmonella typhimurium intraperitoneally. Similarly, the animals were infected with Coxsackie B3 test culture virus (dose 106 TCD50). The sensitivity of mice to Coxsackie B and salmonella viruses was examined for mortality and disease characteristics. The animals were removed from the experiment 24 h after infection, electronically microscopically studied structural and morphological changes of the internal organs were performed. There was no statistically significant difference in morbidity (23.33-26.66 %) and mortality (16.66-20.0 %) of mice infected with Coxsackie virus with dysbiotic disorders and preserved microflora. Dysbiotic conditions have been shown to lead to associated viral-bacterial infections in animals and, accordingly, an increase in the incidence of disease and death in experimental animals. Against the background of disturbance of the composition of the normal intestine microbiota in viral bacterial infections, pronounced degenerative changes in the internal organs of animals were established, with signs of generalization. Electrogram data showed the appearance of activation of immunocompetent cells of the body in viral-bacterial infection in animals with impaired intestine microbiocenosis.

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Section: Discussionmentioning

confidence: 99%

Modeling of viral-bacterial infections against antibiotic-induced intestinal dysbiosis

Bobyr¹,

Stechenko²,

Shyrobokov³

et al. 2019

Rep. of Morph.

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“…The ‘Animal Research: Reporting in-Vivo Experiments’ (ARRIVE) [64] and the Schedule Of Enrolment, Interventions, And Assessments (SPIRIT) reporting guidelines [65] have been considered and integrated into this study protocol were applicable (see also Additional file 2) .…”

Section: Methods and Study Designmentioning

confidence: 99%

A double-blinded randomised dietary supplement crossover trial design to investigate the short-term influence of medium chain fatty acid (MCT) supplement on canine idiopathic epilepsy: study protocol

et al. 2019

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Background Epilepsy is the most common brain disease in dogs. Recently, diets have been reported to have a positive impact on seizure activity and behaviour in various species including dogs with idiopathic epilepsy (IE). Historically, classic high fat ketogenic diets (KD) and medium chain triglycerides (MCT) KD have been successfully used to manage drug-resistant epilepsy. Similarly, an MCT enriched diet has been shown to improve seizure control and behavioural comorbidities in some dogs with IE. However, it is unknown whether an MCT dietary supplement (DS) may provide similar positive effects. Methods A 6-month prospective, randomised, double-blinded, placebo-controlled, crossover, multicentre dietary trial is designed comparing a 9% metabolic energy based calculated medium-chain triglyceride (MCT) oil supplement to a conventional ‘control’ DS. Only dogs which will have an International Veterinary Epilepsy Task Force Tier II level like diagnosis of IE which satisfied the following inclusion criteria are included: age between 6 months and ≤ 12 years; weighing between 4 and ≤ 65 kg; unremarkable interictal neurological examinations; no clinically significant findings on routine laboratory diagnostics; unremarkable brain MRI scan; have had at least 3 seizures in the previous 3 months prior to enrolment; treated with at least one ASD and being classified as resistant. All dogs are fed initially for 90 ± 2 days with either the control oil or the MCT oil alongside their normal diet, followed by 97 ± 2 days with the other supplement including a 7-day washout period. Overall, the aim is to recruit thirty-six patients at five different centres and to investigate the effect of MCTs as DS on seizure activity, tolerability, behavioural comorbidities and quality of life (QoL). Discussion Dietary interventions are rarely studied in a standardised form in veterinary medicine. The background diet, the cohort of animals and ASD received is standardised in this prospective diet trial to ensure representative data about the potential effect of MCT DS. If the study data confirms former findings, this would provide further evidence for the efficacy of MCTs as a management option for canine epilepsy. This publication should offer a repository of trial conditions and variable description with forecasted statistical analysis. Electronic supplementary material The online version of this article (10.1186/s12917-019-1915-8) contains supplementary material, which is available to authorized users.

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“…These include (1) that in spite of a reasonable number of studies on the topic, there were often few studies examining the same pairwise comparison; (2) there was a lack of standardised outcome measures relevant to well-being and timepoints for comparison; (3) many studies were at high risk of bias, either by virtue of study design or deficiencies in reporting. When considering that there have been significant and repeated recent efforts to improve the reporting standards of animal research, and that guidelines to assist animal research have been widely available since 2010 [63][64][65][66], the overall poor quality of reporting of the included studies is problematic. Simple details prescribed by the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines, such as description of the randomisation procedure, were only reported by three studies published after 2010 (of 16 total).…”

Section: Evidence Completeness and Quality And Recommendations For Fumentioning

confidence: 99%

The Impact of Common Recovery Blood Sampling Methods, in Mice (Mus Musculus), on Well-Being and Sample Quality: A Systematic Review

Whittaker

Barker

2020

Animals

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Blood sampling is often performed in laboratory mice. Sampling techniques have the potential to cause pain, distress and impact on lifetime cumulative experience. In spite of institutions commonly providing guidance to researchers on these methods, and the existence of published guidelines, no systematic evaluation of the evidence on this topic exists. A systematic search of Medline, Scopus, and Web of Science was performed, identifying 27 studies on the impact of recovery blood sample techniques on mouse welfare and sample quality. Studies were appraised for quality using the SYstematic Review Centre for Laboratory animal Experimentation (SYRCLE) risk of bias tool. In spite of an acceptable number of studies being located, few studies examined the same pairwise comparisons. Additionally, there was considerable heterogeneity in study design and outcomes, with many studies being at a high risk of bias. Consequently, results were synthesised using the Synthesis Without Meta-analysis (SWiM) reporting guidelines. Grading of Recommendations, Assessment, Development and Evaluation (GRADE) was utilised for assessment of certainty in the evidence. Due to the heterogeneity and GRADE findings, it was concluded that there was not enough high-quality evidence to make any recommendations on the optimal method of blood sampling. Future high-quality studies, with standardised outcome measures and large sample sizes, are required.

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Improving animal research reporting standards

Abstract: The HAARP guidelines aim to set a global minimum standard for reporting results from and details of research experiments using animals. Their adoption would contribute to more transparency in research and improve reproducibility.

Cited by 41 publications

References 11 publications

Modeling of viral-bacterial infections against antibiotic-induced intestinal dysbiosis

Modeling of viral-bacterial infections against antibiotic-induced intestinal dysbiosis

A double-blinded randomised dietary supplement crossover trial design to investigate the short-term influence of medium chain fatty acid (MCT) supplement on canine idiopathic epilepsy: study protocol

The Impact of Common Recovery Blood Sampling Methods, in Mice (Mus Musculus), on Well-Being and Sample Quality: A Systematic Review

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