Improving arterial prosthesis neo-endothelialization: Application of a proactive VEGF construct onto PTFE surfaces

Crombez, M.; Chevallier, Pascale; C.‐Gaudreault, René; Petitclerc, Éric; Mantovani, Diego; Laroche, Gaétan

doi:10.1016/j.biomaterials.2005.05.051

Cited by 93 publications

(76 citation statements)

References 33 publications

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“…This 42 kDa glycoprotein is involved in all phases of neovascularization, including enzymatic degradation of the extracellular matrix, migration and proliferation of endothelial cells, and organization of the cells into tubules. Several groups have investigated use of VEGF for controlling tissue repair and tissue-biomaterial interactions in applications such as arterial graft endothelialization [12], bone grafting [13], in-stent intimal formation [14], and limb [15] and myocardial ischemia [16].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of glycoproteins, such as VEGF, on biodegradable substrates

Sharon

Puleo

2008

Acta Biomaterialia

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Attachment of growth factors to biodegradable polymers, such as poly(lactide-co-glycolide) (PLGA), may enhance and/or accelerate integration of tissue engineering scaffolds. Although proteins are commonly bound via abundant amino groups, a more selective approach may increase bioactivity of immobilized molecules. In this research, exposed carboxyl groups on acid-terminated PLGA were modified with dihydrazide spacer molecules. The number of hydrazide groups available for subsequent attachment of protein was dependent on dihydrazide length, with shorter molecules present at significantly greater surface densities. The potent angiogenic glycoprotein vascular endothelial growth factor (VEGF) was oxidized with periodate and the aldehyde moieties allowed to react with the hydrazide-derivatized PLGA. Derivatization initially affected the amount of protein bound to the surfaces, but differences were substantially reduced following overnight incubation in saline. More importantly, use of shorter dihydrazide spacers significantly enhanced accessibility of immobilized VEGF for binding neutralizing antibody and soluble VEGR receptor. Furthermore, immobilized growth factor enhanced endothelial cell proliferation, with surfaces having the shortest and longest spacers stimulating greater effects. The present work has not only demonstrated an alternative approach to immobilizing growth factors on biodegradable materials, but the scheme can be used to alter the amount of protein bound as well as its availability for subsequent biointeractions.

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Section: Introductionmentioning

confidence: 99%

Immobilization of glycoproteins, such as VEGF, on biodegradable substrates

Sharon

Puleo

2008

Acta Biomaterialia

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“…ELISA is a common method to characterize GF immobilization. [5,8,17,50] However, in our case, the signal collected in direct ELISA was only semi quantitative since a saturation was observed for incubation concentrations higher than 5 nM and 1 nM for E-EGF and E-VEGF, respectively (Fig. 2).…”

Section: Discussionmentioning

confidence: 84%

“…2). Several studies using a similar direct ELISA method did not report any saturation of the signal, [17,50,51] while in contrast, Shen et al also observed a detection limit of direct ELISA for samples with high quantities of covalently immobilized VEGF in a collagen scaffold. [8] Our first hypothesis is that the saturation was due to steric hindrance of anti-EGF antibodies, anti-VEGF antibodies or horseradish peroxidase enzyme.…”

Section: Discussionmentioning

confidence: 98%

Controlled co-immobilization of EGF and VEGF to optimize vascular cell survival

et al. 2016

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Growth factors (GFs) are potent signaling molecules that act in a coordinated manner in physiological processes such as tissue healing or angiogenesis. Co-immobilizing GFs on materials while preserving their bioactivity still represents a major challenge in the field of tissue regeneration and bioactive implants. In this study, we explore the potential of an oriented immobilization technique based on two high affinity peptides, namely the Ecoil and Kcoil, to allow for the simultaneous capture of the epidermal growth factor (EGF) and the vascular endothelial growth factor (VEGF) on a chondroitin sulfate coating. This glycosaminoglycan layer was selected as it promotes cell adhesion but reduces non-specific adsorption of plasma proteins. We demonstrate here that both Ecoiltagged GFs can be successfully immobilized on chondroitin sulfate surfaces that had been pre-decorated with the Kcoil peptide. As shown by direct ELISA, changing the incubation concentration of the various GFs enabled to control their grafted amount.Moreover, cell survival studies with endothelial and smooth muscle cells confirmed that our oriented tethering strategy preserved GF bioactivity. Of salient interest, coimmobilizing EGF and VEGF led to better cell survival compared to each GF captured alone, suggesting a synergistic effect of these GFs. Altogether, these results demonstrate the potential of coiled-coil oriented GF tethering for the co-immobilization of macromolecules; it thus open the way to the generation of biomaterials surfaces with fine-tuned biological properties.

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“…For instance, surfaces of PCL [142,150], PLLA [147], PA [18], PE [137], and PET [151] were provided with oxygen containing functional groups by oxygen plasma exposition. Moreover, carbon dioxide plasma could be used to introduce carboxyl groups to PP [152], PS [153], and PE [152]. The generation of amine groups was in addition performed by ammonia plasma to PTFE [154] and PS [153].…”

Section: Techniques In Surface Activationmentioning

confidence: 99%

Polymers in Cardiology

Sternberg¹,

Busch

Petersen

2014

Advanced Polymers in Medicine

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Polymers have found widespread applications in cardiology, in particular in coronary vascular intervention as stent platforms, coating matrices for drug-eluting stents (DES) and drug-coated balloons (DCB) and for transcatheter valve therapy. Besides permanent polymers, biodegradable polymers came in focus of current research and development for medical applications, as they degrade once their function is fulfilled, which might efficiently reduce observed hypersensitivity reactions. After reviewing polymers used for cardiovascular applications, the book chapter deals with possible surface modification reactions of the polymers including the provision with a local drug delivery function and/ or biofunctionalization in order to selectively control cell-implant interactions. These functionalizations can be furthermore designed to enhance bio-and hemocompatibility, which is of special interest for cardiovascular implants and devices. A general discussion of bio-and hemo-compatibility of polymers for cardiovascular applications and corresponding evaluation methods is additionally given. With our own published data, we finally highlight exemplary polymer applications in cardiology as polymer-based biodegradable stent platforms, biodegradable polymeric coatings for DES and hydrogel-based coatings for DCB. Moreover, developed biofunctionalization strategies of polymers are discussed with regard to their application in cardiology.

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Improving arterial prosthesis neo-endothelialization: Application of a proactive VEGF construct onto PTFE surfaces

Cited by 93 publications

References 33 publications

Immobilization of glycoproteins, such as VEGF, on biodegradable substrates

Immobilization of glycoproteins, such as VEGF, on biodegradable substrates

Controlled co-immobilization of EGF and VEGF to optimize vascular cell survival

Polymers in Cardiology

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