Improving Aspergillus niger seed preparation and citric acid production by morphology controlling-based semicontinuous cultivation

Jin, Sai; Sun, Fuxin; Hu, Zhijie; Liu, Long; Li, Jianghua; Du, Guocheng; Li, Youran; Shi, Guiyang; Chen, Jian

doi:10.1016/j.bej.2021.108102

Cited by 13 publications

(3 citation statements)

References 39 publications

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“…More than 99% of citric acid in the world is synthesized by biological fermentation. Microbial fermentation is the most important method of industrial production, and about 99% of the products in the world are produced by fermentation (Jin et al, 2021).…”

Section: Application Of Citric Acid In Food Industrymentioning

confidence: 99%

Biomanufacturing of food-grade citric acid and comprehensive utilization of its production wastewater

et al. 2023

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Citric acid is an important and multi-functional organic acid, which is widely used in food, medicine, chemical industry and other fields. It is the largest edible organic acid in the world. Aspergillus niger is an important strain for biosynthesis of citric acid, which is mainly produced by simultaneous saccharification and fermentation with starch raw materials. The reuse of citric acid wastewater is the development direction of comprehensive utilization of citric acid wastewater. In the future, with the combination of novel technology strategies such as gene editing technology and synthetic biology technology, the establishment of fermentation strategies with high yield, high yield, high production intensity and clean production is the development direction to realize efficient green manufacturing in citric acid industry.

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Section: Application Of Citric Acid In Food Industrymentioning

confidence: 99%

Biomanufacturing of food-grade citric acid and comprehensive utilization of its production wastewater

et al. 2023

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“…Conversely, Aspergillus nidulans, existing in a dispersed mycelial form, demonstrates enhanced penicillin production [8]. Research by Sai jin et al indicates that small mycelial pellets formed by Aspergillus niger as dispersed mycelial fragments yield higher citric acid compared to undispersed pellets [9]. Various cultivation factors, including pH, inoculum, nutrients, and trace metals, also intricately affect mycelial morphology.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Monascus Pellet Formation for Improved Secondary Metabolite Production

Zhang,

Liu,

Zhang

et al. 2023

JoF

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Filamentous fungi are well-known for their ability to form mycelial pellets during submerged cultures, a characteristic that has been extensively studied and applied. However, Monascus, a filamentous saprophytic fungus with a rich history of medicinal and culinary applications, has not been widely documented for pellet formation. This study aimed to investigate the factors influencing pellet formation in Monascus and their impact on citrinin production, a key secondary metabolite. Through systematic exploration, we identified pH and inoculum size as critical factors governing pellet formation. Monascus exhibited optimal pellet growth within the acidic pH range from 5 to 6, resulting in smaller, more homogeneous pellets with lower citrinin content. Additionally, we found that inoculum size played a vital role, with lower spore concentrations favoring the formation of small, uniformly distributed pellets. The choice of carbon and nitrogen sources also influenced pellet stability, with glucose, peptone, and fishmeal supporting stable pellet formation. Notably, citrinin content was closely linked to pellet diameter, with larger pellets exhibiting higher citrinin levels. Our findings shed light on optimizing Monascus pellet formation for enhanced citrinin production and provide valuable insights into the cultivation of this fungus for various industrial applications. Further research is warranted to elucidate the molecular mechanisms underlying these observations.

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“…In addition, A. niger has played a significant role in industrial fermentation and has received increasing attention in the liquefaction, alcohol, and glucose manufacturing industries [24]. It can decompose organic matter to produce various organic acids, including citric acid [25,26], tartaric acid [27], and gallic acid [16]. Our group obtained a strain of A. niger, designated as M13, which is resistant to inhibitors and can grow in the undetoxified acid hydrolysates from corn straw.…”

Section: Introductionmentioning

confidence: 99%

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

Yin,

Wang,

et al. 2023

Fermentation

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The biological detoxification of lignocellulose hydrolysate is an effective method through which to enhance microbial fermentation efficiency. In this study, an inhibitor-tolerant strain of A. niger (Aspergillus niger) was used for the biological detoxification of corncob hydrolysate. The results showed that A. niger M13 can tolerate a concentration of at least 7.50 ± 0.19 g/L of acetic acid, 1.81 ± 0.13 g/L of furfural, and 1.02 ± 0.10 g/L of HMF (5-Hydroxymethylfurfural). The spores had a higher detoxification efficiency than the mycelial pellets with a detoxification rate of 0.1566 g/L/h, 0.1125 g/L/h, and 0.015 g/L/h for acetic acid, furfural, and HMF, respectively. The cell preferentially consumed furfural, then HMF, before simultaneously degrading acetic acid and glucose. A. niger M13 spores could accumulate small amounts of citric acid directly from undetoxified hydrolysate at a concentration of about 6 g/L. Therefore, A. niger M13 can serve as an excellent biological detoxification strain and a potential citric acid fermenting strain when using undetoxified lignocellulosic hydrolysates.

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Improving Aspergillus niger seed preparation and citric acid production by morphology controlling-based semicontinuous cultivation

Cited by 13 publications

References 39 publications

Biomanufacturing of food-grade citric acid and comprehensive utilization of its production wastewater

Biomanufacturing of food-grade citric acid and comprehensive utilization of its production wastewater

Enhancing Monascus Pellet Formation for Improved Secondary Metabolite Production

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

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