In this study, the effect of microwave (MW) irradiation at a target temperature of 80 °C with a heating rate of two °C/min and a holding time of five minutes on the physicochemical properties of activated sludge was studied. The results showed that subjecting biosludge to MW pretreatment resulted in a reduction in particle size to 14.5, 44.6, and 72.8 for d10, d50, and d90, respectively and an increase in the specific surface area of the biosludge to 235.9 m<sup>2</sup>/kg. Pretreatment resulted in an increase in soluble oxygen demand, sugar, and protein concentrations to 2,380.0 mg/L, 66.5 mg/L, and 1.81 g/L, respectively. A slight increase in the release of inorganic compounds, especially NH<sub>4</sub>-N and PO<sub>4</sub>-P, was observed in the disintegrated biosludge. While there was an increase in release of K and Zn, a decrease in concentrations of Na, Ca, Mg, and Al in the liquid was observed after MW irradiation. It was determined that the dewatering property deteriorated after biosludge disintegration, as it increased from raw sludge to disintegrated sludge by 21-fold.