Improving biosynthetic production of pinene through plasmid recombination elimination and pathway optimization

Bao, Shaoheng; Zhang, Dongyi; Meng, Er

doi:10.1016/j.plasmid.2019.102431

Cited by 19 publications

(16 citation statements)

References 23 publications

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“…An E. coli strain with the genes recA, recE, recF, recI and endA removed was transformed with a plasmid encoded for pinene production with strong terminators. This E. coli strain increased pinene titer by 6.9 fold [36].…”

Section: E Colimentioning

confidence: 88%

“…There are several challenges related to increasing E. coli-produced pinene titers. The heterologous enzymes introduced to E. coli for pinene production produce intermediates that cannot be utilized by the E. coli cell and this metabolic burden can cause plasmid instability [36]. Plasmid recombination can be reduced and pinene titer increased by altering the transcriptional rrnB-T1 terminators on the plasmid to strong terminators that are not homologous [36].…”

Section: E Colimentioning

confidence: 99%

“…The heterologous enzymes introduced to E. coli for pinene production produce intermediates that cannot be utilized by the E. coli cell and this metabolic burden can cause plasmid instability [36]. Plasmid recombination can be reduced and pinene titer increased by altering the transcriptional rrnB-T1 terminators on the plasmid to strong terminators that are not homologous [36]. An E. coli strain with the genes recA, recE, recF, recI and endA removed was transformed with a plasmid encoded for pinene production with strong terminators.…”

Section: E Colimentioning

confidence: 99%

“…The catalytic process of mevalonic kinase and mevalonate diphosphate decarboxylase were identified as the bottlenecks of the MVA pathway in recombinant E. coli [36]. A strain of laboratory involved pinene-producing E. coli strain was whole genome sequenced, then aligned with the E. coli reference strain MG1655 genome sequence, then non-synonymous mutant genes were up and downregulated [37].…”

Section: E Colimentioning

confidence: 99%

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The Potential Production of the Bioactive Compound Pinene Using Whey Permeate

et al. 2020

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Pinene is a secondary plant metabolite that has functional properties as a flavor additive as well as potential cognitive health benefits. Although pinene is present in low concentrations in several plants, it is possible to engineer microorganisms to produce pinene. However, feedstock cost is currently limiting the industrial scale-up of microbial pinene production. One potential solution is to leverage waste streams such as whey permeate as an alternative to expensive feedstocks. Whey permeate is a sterile-filtered dairy effluent that contains 4.5% weight/weight lactose, and it must be processed or disposed of due its high biochemical oxygen demand, often at significant cost to the producer. Approximately 180 million m3 of whey is produced annually in the U.S., and only half of this quantity receives additional processing for the recovery of lactose. Given that organisms such as recombinant Escherichia coli grow on untreated whey permeate, there is an opportunity for dairy producers to microbially produce pinene and reduce the biological oxygen demand of whey permeate via microbial lactose consumption. The process would convert a waste stream into a valuable coproduct. This review examines the current approaches for microbial pinene production, and the suitability of whey permeate as a medium for microbial pinene production.

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Section: E Colimentioning

confidence: 88%

Section: E Colimentioning

confidence: 99%

Section: E Colimentioning

confidence: 99%

Section: E Colimentioning

confidence: 99%

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The Potential Production of the Bioactive Compound Pinene Using Whey Permeate

et al. 2020

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“…the tyre-derived oil has a significantly high concentration of DL-limonene [19,20]. On an industrial scale, a,b-pinenes are mainly obtained as byproducts of paper pulping or extracted from turpentine; new alternatives include the production of pinenes by bioprocesses using E. coli systems [21,22].…”

Section: Introductionmentioning

confidence: 99%

Techno‐Economic Evaluation of d‐Limonene and α‐Pinene Separation from Citrus and Turpentine Oils

Becerra

Villa

2020

Chem Eng & Technol

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The evaluation of the technical and economic separation of the monoterpenes a-pinene and d-limonene from essential oils, carried out by computational simulation of the vacuum fractional distillation in continuous and batch mode from samples of turpentine and citrus oils, was developed using Aspen Plus software combined with estimations of operational expenses (OPEX) and capital expenses (CAPEX) based on the Guthrie method. The production of d-limonene and a-pinene with high purity from the essential oils is a technically feasible process. From the economic point of view and based on the average annual import costs of turpentine and orange oils in Colombia, it was found that the selling prices of a-pinene and d-limonene obtained from the distillation module cost analysis depend on the daily production and can be minimized if essential oils are preliminary extracted from local industrial wastes.

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What We Can Learn from Measuring Metabolic Fluxes in Cyanobacteria

Gao

Cantrell

et al. 2021

Cyanobacteria Biotechnology

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Improving biosynthetic production of pinene through plasmid recombination elimination and pathway optimization

Cited by 19 publications

References 23 publications

The Potential Production of the Bioactive Compound Pinene Using Whey Permeate

The Potential Production of the Bioactive Compound Pinene Using Whey Permeate

Techno‐Economic Evaluation of d‐Limonene and α‐Pinene Separation from Citrus and Turpentine Oils

What We Can Learn from Measuring Metabolic Fluxes in Cyanobacteria

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