Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection

Liu, Wuge; Jin, Sujuan; Zhu, Xiaoyuan; Feng, Wang; Li, Jinhua; Liu, Zhenrong; Liao, Yuncheng; Zhu, Min; Huang, Huijun; Yi-bai, Liu

doi:10.1016/s1672-6308(08)60040-2

Cited by 9 publications

(2 citation statements)

References 5 publications

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“…Pi25 GM2 has been successfully utilized in modern breeding to improve the blast resistance of elite rice parents, hybrids, and conventional varieties [10][11][12][13][14][15][16]22,27]; a low frequency of the R allele was found in the germplasm in China [9,[17][18][19][20][21], implying Pi25 GM2 is rare. Furthermore, little is known about the distribution of Pi25 GM2 abroad, which would largely limit the utilization of this important gene.…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, CAP3/Bgl II and CAP3/Nde I could digest specifically the S allele Pi25 ZJ100 , according to SNP 2444 and SNP 2566, respectively [9]. These markers were used to improve the blast resistance of varieties [15,16]. Furthermore, they were also utilized to clarify the distribution of the R allele of the Pi25 gene in rice germplasm in China [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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A Novel PCR-Based Functional Marker of Rice Blast Resistance Gene Pi25

Fan,

Zhang,

Huang

et al. 2023

Agriculture

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Rice blast is arguably the most devastating fungal disease of rice. Utilization of resistance genes to breed resistant cultivars is one of the most economical and environmentally friendly approaches to combat the disease. Pi25, a major resistance gene conferring broad-spectrum resistance to both leaf and neck blast, is an ideal gene resource to improve the resistance of rice varieties to blast. Recently, several allele-specific markers were developed. However, they were deficiently efficient due to either an additional process of restriction enzyme digestion for cleaved amplified polymorphic sequence (CAPS) markers or the risk of false-positive error in identifying susceptible Tetep allele (Pi25TTP) for PCR-based markers. In this study, based on a conserved single nucleotide polymorphism (SNP) between resistant and susceptible alleles, a tetra-primer amplification refractory mutation system (ARMS)-PCR marker was developed. The new marker, namely Pi25-2687R3, could effectively distinguish the resistant Gumei 2 (GM2) allele (Pi25GM2) and the susceptible allele Pi25TTP. Moreover, a perfect consistency of genotyping was exhibited between Pi25-2687R3 and published CAPS marker CAP3/Hpy 99I. A more accurate genotyping was also displayed compared to the previous PCR-based SNP marker Pi25-2566. Our finding proved that Pi25-2687R3 could achieve the same result as CAP3/Hpy 99I with less workload and cost and could promote the accuracy in the identification of genotypes superior to Pi25-2566. This study provided a quick and reliable functional marker for discriminating Pi25 alleles, which would be a valuable tool for genotypic assay and rice molecular breeding of blast resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel PCR-Based Functional Marker of Rice Blast Resistance Gene Pi25

Fan,

Zhang,

Huang

et al. 2023

Agriculture

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Molecular Approaches for Disease Resistance in Rice

et al. 2021

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Rice production needs to be sustained in the coming decades, with changing climatic conditions becoming more conducive to the prevalence of disease outbreaks. Major rice diseases collectively cause enormous economic damage and yield instability. Breeding for disease-resistant rice varieties could be one of the best options to counter these disease outbreaks. Disease-screening protocols and newer technologies are essential for effective phenotyping and would aid in gene discovery and function. Understanding the genetics of disease mechanisms and stacking of broad-spectrum disease-resistance genes could lead to faster development of rice varieties with multiple disease resistance. New molecular breeding approaches are discussed for the development of these varieties. The molecular biology of disease resistance is now better understood and could be well manipulated for improved resilience. Transgenic approaches for disease resistance are discussed. Genome-editing tools for the development of disease-resistant rice varieties are thoroughly discussed. The use of bioinformatics tools to speed up the process and to obtain a better understanding of molecular genetics mechanisms of disease resistance is explained.

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Genetic mapping of a QTL controlling leaf width and grain number in rice

Tian

Hong-yu

et al. 2014

Euphytica

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Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection

Cited by 9 publications

References 5 publications

A Novel PCR-Based Functional Marker of Rice Blast Resistance Gene Pi25

A Novel PCR-Based Functional Marker of Rice Blast Resistance Gene Pi25

Molecular Approaches for Disease Resistance in Rice

Genetic mapping of a QTL controlling leaf width and grain number in rice

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