Improving carboxymethyl cellulose edible coating using ZnO nanoparticles from irradiated Alternaria tenuissima

Abstract

In this paper, gamma-irradiation was successfully used to intensify the yield of Zinc oxide nanoparticles (ZnONPs) produced by the fungus Alternariatenuissima as a sustainable and green process. The obtained data showed that 500 Gy of gamma-irradiation increased ZnONPs’ yield to approximately four-fold. The synthesized ZnONPs were then exploited to develop active Carboxymethyl Cellulose films by casting method at two different concentration of ZnONPs 0.5% and 1.0%. The physicochemical, mechanical, antioxidant, and antimicrobial properties of the prepared films were evaluated. The incorporation of ZnONPs in the Carboxymethyl Cellulose films had significantly decreased solubility (from 78.31% to 66.04% and 59.72%), water vapor permeability (from 0.475 g m−2 to 0.093 g m−2 and 0.026 g m−2), and oxygen transfer rate (from 24.7 × 10–2 to 2.3 × 10–2 and 1.8 × 10–2) of the respective prepared films. Meanwhile, tensile strength (from 183.2 MPa to 203.34 MPa and 235.94 MPa), elongation (from 13.0% to 62.5% and 83.7%), and Yang's modulus (from 325.344 to 1410.0 and 1814.96 MPa) of these films were increased. Moreover, the antioxidant and antimicrobial activities against several human and plant pathogens the prepared of Carboxymethyl Cellulose-ZnONPs films were significantly increased. In conclusion, the prepared Carboxymethyl Cellulose-ZnONPs films showed enhanced activities in comparison with Carboxymethyl Cellulose film without NPs. With these advantages, the fabricated Carboxymethyl Cellulose-ZnONPs films in this study could be effectively utilized as protective edible coating films of food products.

Effects of dietary supplementation of myco-fabricated zinc oxide nanoparticles on performance, histological changes, and tissues Zn concentration in broiler chicks

“…Fungal spores from the cultures (7 days old) of A. tenuissima were harvested and the spore concentration was adjusted to a concentration of 10 6 spores/ml. Spore suspension was transferred in vial sealed with paraffin and irradiated by gamma rays at a dose of 500 Gy, the best irradiation dose for maximum synthesis of ZnONPs 23 . Irradiation process was carried out at the Nuclear Research Center (Cairo, Egypt) using 60 Co Gamma chamber, MC20, Russia, with an average dose rate of 605.726 Gy h −1 at the time of the experiment.…”

Gamma irradiation mediated production improvement of some myco-fabricated nanoparticles and exploring their wound healing, anti-inflammatory and acetylcholinesterase inhibitory potentials

“…1 C) showed that the crystal structure was hexagonal with a lattice parameter of 0.323110 nm which is in agreement with JCPDS card No. 361451 4 , 21 , 30 . It is noteworthy that, all the myco-fabricated NPs are pure and of a single phase (Fig.…”

“…To induce mutants with improved productivities of CoFeNPs, SeNPs, and ZnONPs, spore suspensions of Monascus purpureus ATCC16436 and Alternaria tenuissima AUMC10624 were separately prepared. According to previous studies 2 , 3 , 21 , 1000 Gy (for CoFeNPs and SeNPs) and 500 Gy (ZnONPs) of gamma rays were the most proper doses for maximum production of these NPs, so we used the same irradiation doses in this paper to isolate mutant strains with improved productivities. The irradiation process was performed using a 60 Co Gamma chamber (MC20, Russia) at the Nuclear Research Center, Egyptian Atomic Energy Authority.…”

“…Finally, the highest NPs-producing mutant strains ( M. purpureus Mutant-2022 and A. tenuissima Mutant-1985) were followed up for their stability across six successive generations, under the conditions described earlier. The yield of NPs was estimated by weighing the obtained powders from every irradiation dose and expressed as mg NPs mL −1 2 , 3 , 21 .…”

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

“…Previous reports concluded the effect of gamma rays as an energetic ionizing radiation in induction of mutations in the exposed cells (Thacker 1999) through the repair mechanism in genes of the DNA of the irradiated cells (Ismaiel et al 2014(Ismaiel et al , 2015Zaki et al 2020;Zaki and El-Sayed 2021). Generally, exposure to physical mutagens such as gamma rays is a successful strategy to improve microbial strains by developing overproducers (Parekh et al 2000) with intensified production rates (El-Sayed et al 2020a, c;Zaki et al 2020) with industrial applications thereby effectively lowering the overall cost of the process, as supported by several reports (Anwar et al 2022;El-Sayed et al 2020d, e, f;El-Sayed 2021;El-Sayed et al 2022b;c).…”