Improving chestnut physicochemical properties through fermentation – Development of chestnut Amazake

Carbon stable isotopes of glucose during the degradation of rice by the koji fungus Aspergillus oryzae

“…The observed higher conversion rate at 50 °C (∼60 % after 24 h) than at 15 °C (∼10 % after 24 h) ( Fig. 3 c) aligns with the reported optimal activity of α-amylase from A. oryzae at ∼50 °C [ 11 , 27 , 38 , 39 ], highlighting the importance of temperature optimization for maximizing enzymatic conversion rates. Interestingly, our results suggest that the final post-saccharification glucose concentration is determined mainly by the degradation rate of the rice koji, rather than the specific temperature and reaction time utilized ( Table 2 ).…”

“…This study aimed to quantify carbon isotope discrimination from rice to glucose during the saccharification of rice koji, a process identical to that used for koji amazake, a sweet non-alcoholic fermented beverage made with rice koji and water [ 11 , 12 , 27 ]. We hypothesized that the δ 13 C value of glucose might differ from that of the rice source, with the difference being controlled by the degradation rate.…”

Harnessing Bacillus amyloliquefaciens for Amazake Production: Comparison with Aspergillus oryzae Amazake for Metabolomic Characteristics, Microbial Diversity, and Sensory Profile

“…Proteases break down proteins into peptides, which can contribute flavor precursors, mouthfeel, and enhance the α-amylase activity [34]. We measured the protease activity to better understand the dynamics of the fermentation.…”

Physiological response of chestnuts (Castanea mollissima Blume) infected by pathogenic fungi and their correlation with fruit decay

“…Chestnuts ( Castanea mollissima Blume ) are a popular forest food crop in Asia, Europe, and America due to their nutritional value, unique taste, and pleasant aroma ( Massantini, Moscetti, & Frangipane, 2021 ). Chestnut fruit is rich in starch, protein, functional polysaccharides, essential fatty acids, vitamins, and minerals ( Santos et al, 2023 ). Additionally, chestnuts have various health benefits such as lowering cholesterol, preventing obesity and diabetes, fighting tumours, and improving the immune system ( Li et al, 2022a ).…”