Improving d-mannitol productivity of Escherichia coli: Impact of NAD, CO2 and expression of a putative sugar permease from Leuconostoc pseudomesenteroides

Heuser, Florian; Marin, Kay; Kaup, Björn; Bringer, Stephanie; Sahm, Hermann

doi:10.1016/j.ymben.2009.01.006

Cited by 15 publications

(5 citation statements)

References 22 publications

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“…In resting cells of E. coli BL21 (DE3) three genes were simultaneously expressed such as mdh, fdh and glf which encodes mannitol, formate dehydrogenase and a sugar facilitator, respectively, and thus the productivity of d-mannitol formation obtained with the strain expressing the additional fupL gene was enhanced by 20%. In an expression system a fusion protein was prepared for mannitol-2-dehydrogenase mtlK in pRSET vector in E. coli BL21pLysS on isopropyl-~-D-thiogalactopyranoside induction system for mannitol formation at pH 5.35 [ 60 ]. Similarly genetically engineered L. plantarum TF103 carrying the mtlK gene of L. brevis overexpressor reported to produce mannitol from glucose.…”

Section: Factors Affecting Mannitol Productionmentioning

confidence: 99%

Challenges in Enzymatic Route of Mannitol Production

2013

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Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

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Section: Factors Affecting Mannitol Productionmentioning

confidence: 99%

Challenges in Enzymatic Route of Mannitol Production

2013

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“…To extract NADH, 250 ml cell cultures were centrifuged at 4600 rcf for 5 min and resuspended in extraction buffer [1 ml of 0.5 M Tris-HCl, pH 7.0 containing 1 mM EDTA and 1 ml of methanol ( Heuser et al, 2009 )]. After the addition of 2 ml of chloroform, NADH extraction was performed by shaking the tube for 1.5 h at 25°C.…”

Section: Methodsmentioning

confidence: 99%

The soluble transhydrogenase UdhA affecting the glutamate-dependent acid resistance system of Escherichia coli under acetate stress

et al. 2018

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The soluble transhydrogenase (UdhA) is one of two transhydrogenases that play a role in maintaining the balance between NAD(H) pools and NADP(H) pools in Escherichia coli. Although UdhA has been extensively used in metabolic engineering and biocatalysis for cofactor regeneration, its role in acid resistance has not been reported. Here we used DNA microarray to explore the impact of UdhA on transcript levels. We demonstrated that during growth on acetate, the expression of genes involved in the respiratory chain and Gad acid resistance system was inhibited in the udhA-knockout strain. The deletion of udhA significantly repressed the expression of six genes (gadA, gadB, gadC, gadE, hdeA and hdeB) which are involved in Gad acid resistance and resulted in low survival of the bacterium at a low pH of 4.9. Moreover, UdhA was essential for NADH production which is important for the adaptive growth of E. coli on acetate, while NADH concentration in the udhA-knockout strain was quite low and supplemental NADH significantly increased the expression of acid resistance genes and survival of the udhA-knockout strain. These results demonstrated that UdhA is an important source of NADH of E. coli growth on acetate and affects Gad acid resistance system under acetate stress.

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“…Finally, the recombinant E. coli BL21 (DE3) expressing glf, fdh and mdh was able to and Gao (1996) High yield High material cost and polluted form 362 mM D-mannitol from an initial 500 mM D-fructose of within 8 h, and the molar yield Y D-mannitol/D-fructose reached 84% and a specific productivity was more than 4 g D-mannitol g −1 (cell dry weight) h −1 (Kaup et al, 2004). In order to overcome the instability of the recombinant strain, which possibly caused by factors such as the internal pH change of the cells, loss of cofactor NAD, high formate concentrations and export of D-mannitol, the fupL gene was additionally expressed in biotransformation experiments, and the final D-mannitol productivity of the strain was enhanced by 20% (Heuser et al, 2009). Secondly, in order to convert D-glucose to D-mannitol, an intracellular glucose isomerase, formate dehydrogenase, D-mannitol dehydrogenase, glucose isomerase, and glucose facilitator were co-expressed in the E. coli strain I, resulting in the production of up to 821 mM D-mannitol at the optimized pH and temperature (Kaup et al, 2005).…”

Section: Synthesis Of D-mannitol In Recombinantmentioning

confidence: 99%

New Insights Into the Biosynthesis of Typical Bioactive Components in the Traditional Chinese Medicinal Fungus Cordyceps militaris

Huang

et al. 2021

Front. Bioeng. Biotechnol.

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Cordyceps militaris, a traditional medicinal ingredient with a long history of application in China, is regarded as a high-value fungus due to its production of various bioactive ingredients with a wide range of pharmacological effects in clinical treatment. Several typical bioactive ingredients, such as cordycepin, D-mannitol, cordyceps polysaccharides, and N6-(2-hydroxyethyl)-adenosine (HEA), have received increasing attention due to their antitumor, antioxidant, antidiabetic, radioprotective, antiviral and immunomodulatory activities. Here, we systematically sorted out the latest research progress on the chemical characteristics, biosynthetic gene clusters and pathways of these four typical bioactive ingredients. This summary will lay a foundation for obtaining low-cost and high-quality bioactive ingredients in large amounts using microbial cell factories in the future.

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Improving d-mannitol productivity of Escherichia coli: Impact of NAD, CO2 and expression of a putative sugar permease from Leuconostoc pseudomesenteroides

Cited by 15 publications

References 22 publications

Challenges in Enzymatic Route of Mannitol Production

Challenges in Enzymatic Route of Mannitol Production

The soluble transhydrogenase UdhA affecting the glutamate-dependent acid resistance system of Escherichia coli under acetate stress

New Insights Into the Biosynthesis of Typical Bioactive Components in the Traditional Chinese Medicinal Fungus Cordyceps militaris

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