Improving development of cloned goat embryos by supplementing α-lipoic acid to oocyte in vitro maturation medium

Zhang, Hengde; Wu, Bin; Liu, Hongliang; Qiu, Mingning; Li, Jun; Zhang, Yong; Quan, Fusheng

doi:10.1016/j.theriogenology.2013.03.027

Cited by 29 publications

(21 citation statements)

References 30 publications

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“…The main challenge resides in the relatively low maturation rate (about 60%) and reproductive outcomes (Nogueira et al 2000;Zhang et al 2013). Optimally matured oocytes will have a great potential to develop into good-quality embryos.…”

Section: Introductionmentioning

confidence: 99%

“…However, current goat oocyte IVM remains suboptimal. The main challenge resides in the relatively low maturation rate (about 60%) and reproductive outcomes (Nogueira et al 2000;Zhang et al 2013). Supplemented with some factors, proteins or hormones play an important role for improving oocyte maturation and subsequent embryo development competence (Armstrong et al 1991;Bavister et al 1992;Zhang et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The main challenge resides in the relatively low maturation rate (about 60%) and reproductive outcomes (Nogueira et al 2000;Zhang et al 2013). Supplemented with some factors, proteins or hormones play an important role for improving oocyte maturation and subsequent embryo development competence (Armstrong et al 1991;Bavister et al 1992;Zhang et al 2013). Vitamin A is one of the fat-soluble vitamins and is generally believed to regulate cell growth and differentiation, tissue function, and support both male and female reproduction as well as embryonic development (Clagett-Dame & Knutson 2011).…”

Section: Introductionmentioning

confidence: 99%

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All‐trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation

Wang

Bian

et al. 2014

Animal Science Journal

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All-trans retinoic acid (t-RA) is a natural component and representative physiologically active metabolite of vitamin A, having multiple physiologic functions. The objective of this study was to evaluate the effect of t-RA on goat oocyte maturation and cumulus cell apoptosis during in vitro maturation (IVM). Immature goat cumulus-oocyte complexes (COCs) were matured in vitro in the absence or presence of t-RA at concentrations of 10 nmol/L, 100 nmol/L and 1000 nmol/L. Oocyte maturation and embryo development were assessed by polar body formation and parthenogenetic activation, respectively. Oocyte survival was checked by Trypan blue staining. Apoptosis of cumulus cells was analyzed by terminal deoxynucleotidyl transferase nick end labeling staining and quantitative real-time PCR. In comparison with the control group, 100 nmol/L and 10 nmol/L t-RA significantly improved goat nuclear oocyte maturation and survival (P < 0.05). Addition of 1000 nmol/L t-RA improved nuclear maturation (P < 0.05), but had no effect on survival of goat oocytes. t-RA had no positive effect on goat parthenogenetic embryonic cleavage, blastocyst formation or total cell numbers. However, t-RA inhibits the apoptosis of cumulus cells (P < 0.01). t-RA treatment up-regulated the expression of B-cell lymphoma 2 (BCL-2), catalase (CAT) (P < 0.05) and down-regulated the expression of Caspase-8 (P < 0.05). In conclusion, t-RA has positive effects on goat oocyte nuclear maturation and reduces apoptotic cumulus cells during IVM.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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All‐trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation

Wang

Bian

et al. 2014

Animal Science Journal

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“…Oocyte activation may also be an important component of the precise reprogramming that involves chromatin configuration, histone composition, methylation and acetylation patterns, and genomic imprinting for further development of the embryos (8). Therefore, oocyte maturation is considered an important step in achieving in vitro embryo production, since it is one of the essential elements that determine the success of nuclear transfer and the subsequent development of cloned embryos (9-11). To circumvent this problem, additional treatments with 6-dimethylaminopurine (6-DMAP), a phosphorylation inhibitor, or cycloheximide (CHX), a protein synthesis inhibitor (12), have been used to induce the inactivation of mitosis promoting factor.…”

Section: Introductionmentioning

confidence: 99%

Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo

Oliveira

Mantovani

Silva

et al. 2014

Braz J Med Biol Res

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The compounds 6-dimethylaminopurine and cycloheximide promote the successful production of cloned mammals and have been used in the development of embryos produced by somatic cell nuclear transfer. This study investigated the effects of 6-dimethylaminopurine and cycloheximide in vitro, using the thiazolyl blue tetrazolium bromide colorimetric assay to assess cytotoxicity, the trypan blue exclusion assay to assess cell viability, the comet assay to assess genotoxicity, and the micronucleus test with cytokinesis block to test mutagenicity. In addition, the comet assay and the micronucleus test were also performed on peripheral blood cells of 54 male Swiss mice, 35 g each, to assess the effects of the compounds in vivo. The results indicated that both 6-dimethylaminopurine and cycloheximide, at the concentrations and doses tested, were cytotoxic in vitro and genotoxic and mutagenic in vitro and in vivo, altered the nuclear division index in vitro, but did not diminish cell viability in vitro. Considering that alterations in DNA play important roles in mutagenesis, carcinogenesis, and morphofunctional teratogenesis and reduce embryonic viability, this study indicated that 6-dimethylaminopurine and cycloheximide utilized in the process of mammalian cloning may be responsible for the low embryo viability commonly seen in nuclear transfer after implantation in utero.

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“…In goats, several reports have demonstrated that factors such α-lipoic acid, activin-A and retinoids enhance embryonic development when supplemented during oocyte maturation and/or embryo culture (Bormann et al, 2003;Chiamenti et al, 2010;Kwong et al, 2012;Zhang et al, 2013;Hammami et al, 2014). Vitamin A and its physiological metabolites, collectively known as retinoids, play important roles in embryonic morphogenesis and reproductive physiology as a mitogenic and differentiation stimulus (Hofmann and Eichele, 1994).…”

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confidence: 99%

Use of retinoids during oocyte maturation diminishes apoptosis in caprine embryos

Conceição

Moura

Ferreira-Silva

et al. 2015

Acta Veterinaria Hungarica

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Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mechanisms governing this phenomenon have not been characterised. The aim of the present study was to evaluate if the incidence of apoptosis is affected by the addition of retinyl acetate (RAc) and 9-cis-retinoic acid (RA) during in vitro maturation (IVM) of caprine oocytes. Embryonic development was recorded on days 3 and 8 post-fertilisation, and apoptosis was measured by caspase activity and DNA fragmentation (TUNEL assay). Control zygotes had lower capacity to cleave and reach the blastocyst stage (24.45 ± 2.32 and 5.32 ± 0.81, respectively) than those of RAc-(29.96 ± 1.62 and 7.94 ± 0.93, respectively) and RA-treated groups (30.12 ± 1.51 and 7.36 ± 1.02, respectively). Oocytes and blastocysts positive for TUNEL assay were more frequent, respectively, in the controls (8.20 ± 0.78, 8.70 ± 1.05) than in RAc (5.60 ± 0.52, 4.80 ± 0.51) and RA (6.40 ± 0.69, 5.40 ± 0.69). Caspase activity did not differ between control oocytes (7.20 ± 0.91), RAc (6.60 ± 0.68) and RA (7.30 ± 0.67), but it was reduced in RAc-(5.05 ± 0.62) and RA-treated blastocysts (5.75 ± 0.22) compared to controls (8.35 ± 0.71). These results indicate that the addition of retinoids during IVM increases the developmental potential of goat embryos with a concomitant reduction in apoptosis rates.

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Improving development of cloned goat embryos by supplementing α-lipoic acid to oocyte in vitro maturation medium

Cited by 29 publications

References 30 publications

All‐trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation

All‐trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation

Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo

Use of retinoids during oocyte maturation diminishes apoptosis in caprine embryos

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