Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution

Perez, Caroline L.; Casciatori, Fernanda Perpétua; Thoméo, João Cláudio

doi:10.1007/s00449-020-02466-7

Cited by 19 publications

(8 citation statements)

References 38 publications

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“…The strategy suggests that reasonable control of the O2 concentration, bed temperature and moisture content can be achieved by manipulating the flow rate of moistened air. As reported by Perez et al [4,5], using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities and solving overheating and gaseous exchanges. However, our results contrast with previous reports by Castro et al, 2015 [84].…”

Section: Effect Of Air Arrangement On Spore and Enzyme Productionmentioning

confidence: 67%

“…A strategy that uses intermittent trickling of water in the bed bioreactor, results in good temperature control, avoiding the bed from drying, preventing substrate compaction, as well as increased Aspergillus ficuum mycelium growth and phytase production [13]. Evaluation of different airflow distribution techniques in a pilot-scale bioreactor by using an inner tube for air supply resulted in more homogenous enzyme production, with higher activities by the fungus, Myceliophthora thermophila I-1D3b [4].…”

Section: Heat Evolution As a Mechanism To Describe Fungal Growthmentioning

confidence: 99%

“…A number of SSB designs exist for SSF, such as semi-continuous plug-flow [2], packed-bed [3][4][5][6][7], tray [8][9][10][11][12], trickle bed [13], multipurpose fixed-bed [14], rotating drum [9,15], horizontal drum [16], column-tray [17], novel deep-bed cubical [18], multi-layer packed-bed [19], column [20], fluidised-bed [21], hexahedral modular [22], SSF reactor (SSFR) [23], horizontal rotary drum [24], continuous counter-current [25], scrapped-drum [26], zymotis packed-bed [27], gas-solid spouted-bed [28], horizontal stirred tank [29], vertical cylindrical shaped (fed-batch SSF) [30] and cylindrical [31] bioreactors. Progress has been made in improving processes at the laboratory level, but research on bioreactor designs and process scale-ups that allow the usage of microorganisms needs further focus [32].…”

Section: Introductionmentioning

confidence: 99%

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Performance of Fungal Growth Through Integrated Gompertz Model and Respiratory Quotient by Solid State Fermentation in Multi-Layer Squared Tray Solid State Bioreactor with Aeration Strategies

Manan

Webb

2021

Preprint

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A newly designed, laboratory-scaled and multi-layer squared tray solid state bioreactor (SSB), was developed and successfully operated in solid state fermentation (SSF) conditions. The bioreactor was divided into eight layers of squared perforated trays. Wheat bran was used as a solid substrate for the growth of Aspergillus awamori and Aspergillus oryzae. The SSB was equipped with an oxygen (O2)/carbon dioxide (CO2) gas analyser and a thermocouple. Continuous on-line monitoring of fungal growth could be performed by indirect methods that measure O2 consumed, production of CO2 and metabolic heat. The advantage of using this method is that there are no tedious and time-consuming sampling processes. The evolution of CO2, which represents an accumulation term, was integrated with time and fitted to the Gompertz model in a log-like equation. The Gompertz model generated values that may be used to stimulate and verify the experimental data. Results strongly suggest that the evolved and accumulated CO2, excellently described fungal growth. Simulated results agreed with experimental results. The respiratory quotient (RQ), which is the ratio of CO2 evolution rate (CER) to O2 uptake rate (OUR), was determined by the gas balance method. CER and OUR confirmed that measurements correlated to fungal activity. Each RQ values can explain the differences of each SFF process carried out. Yet, heat evolved by fungal activity also described fungal growth. The current findings is an excellent pre-liminary experimental work, evidencing that multi-layer squared tray SSB with forced moistened aeration present a promising alternative of instrumented bioreactors for SSF processes.

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Section: Effect Of Air Arrangement On Spore and Enzyme Productionmentioning

confidence: 67%

Section: Heat Evolution As a Mechanism To Describe Fungal Growthmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Performance of Fungal Growth Through Integrated Gompertz Model and Respiratory Quotient by Solid State Fermentation in Multi-Layer Squared Tray Solid State Bioreactor with Aeration Strategies

Manan

Webb

2021

Preprint

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“…Perez et al evaluated two air injection alternatives to the traditional end-to-end aeration one. 41 In the first alternative the total airflow rate was split to be introduced in several locations along the column, while in the second alternative air was introduced through a perforated central tube. Both solutions were effective in keeping the temperature of the cultivation medium close to the ideal values, indicating the feasibility of scaling up this class of bioreactors.…”

Section: Incubation and Predryingmentioning

confidence: 99%

“…Perez et al evaluated two air injection alternatives to the traditional end-to-end aeration one . In the first alternative the total airflow rate was split to be introduced in several locations along the column, while in the second alternative air was introduced through a perforated central tube.…”

Section: Alternative Production Processmentioning

confidence: 99%

Assessment of the Techno-Economic Feasibility of Semicontinuous Production of Metarhizium anisopliae Spores

Ferreira,

Mendes,

Thoméo

2023

Ind. Eng. Chem. Res.

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The significant increase and indiscriminate use of chemical pesticides have led to the development of several environmental problems and adverse effects on human health. Alternatively, bioinsecticides have been successfully used for a variety of crops to control several insect infestations with efficiency and competitive costs. Metarhizium anisopliae have widely been used in sugar cane crops in Brazil, and their use is the most successful enthomopathogen program worldwide. However, the production of this propagule is semiartisanal and presents several technical bottlenecks. In order to intensify this production, all the steps of the process have been revised and more efficient techniques and procedures have been adopted to avoid exogenous contamination and increase the productivity. The most relevant modification in this semicontinuous process is the cultivation of rice in packed-bed solid-state cultivation bioreactors and the extraction of spores in rotating drums, with retention of the spores in cyclones. The economic analysis showed that this process is competitive when compared with the traditional process of spore production and with similar biotechnological processes.

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Addition of spherical‐style packing improves the production of conidia by Metarhizium robertsii in packed column bioreactors

Méndez-González

Figueroa-Montero

Saucedo‐Castañeda

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Conidia from Metarhizium are part of the commercial formulations used worldwide for insect pest control. Among the alternatives proposed to improve conidia production, the use of packed column bioreactors allows controlled processes with higher levels of production and productivity than those obtained with the traditional process in plastic bags. However, problems associated with overheating, low CO2 removal, and inadequate oxygen supply in packed columns limit the scale‐up and operation at an industrial scale. RESULTS Packed densities, up to 1.5 times higher than those obtained in lab‐scale column bioreactors, negatively affected growth and conidia production by Metarhizium robertsii (ENCB‐MG‐81). Therefore, a spherical‐style packing was added to decrease packed density and improve the culture conditions in packed column bioreactors. The addition of spherical‐style packing reduced the axial temperature gradient (up to 50%), increased the maximum CO2 production rate and the total CO2 production (up to 2.5 times), and enhanced the volumetric conidia productivity in packed column bioreactors from 5.28 ± 1.36 to 6.77 ± 0.89 × 108 conidia cm−3. CONCLUSION The use of spherical‐style packing within the culture bed in column bioreactors improves the production of conidia by Metarhizium robertsii, as well as the gas exchange and heat removal from the bed. © 2021 Society of Chemical Industry (SCI).

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Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution

Cited by 19 publications

References 38 publications

Performance of Fungal Growth Through Integrated Gompertz Model and Respiratory Quotient by Solid State Fermentation in Multi-Layer Squared Tray Solid State Bioreactor with Aeration Strategies

Performance of Fungal Growth Through Integrated Gompertz Model and Respiratory Quotient by Solid State Fermentation in Multi-Layer Squared Tray Solid State Bioreactor with Aeration Strategies

Assessment of the Techno-Economic Feasibility of Semicontinuous Production of Metarhizium anisopliae Spores

Addition of spherical‐style packing improves the production of conidia by Metarhizium robertsii in packed column bioreactors

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