In this study, Mucuna flagellipes seed extract was applied in the coagulation-flocculation of produced water (PW). Process parameters such as pH, dosage, and settling time were investigated. Process kinetics was also studied. Instrumental characterization of mucuna seed (MS), mucuna seed coagulant (MSC), and post effluent treatment settled sludge (PTSS) were carried out. The optimum decontamination efficiency of 95 % was obtained at 1 g/L MSC dosage, PW pH of 2, and rate constant of 0.0001 (L/g/s). Characterization results indicated that MS, MSC, and PTSS were of network structure, primitive lattice, and thermally stable. It could be concluded that MSC would be potential biomass for the treatment of produced water under the experimental conditions.