Improving hemocompatibility of artificial lungs by click conjugation of glycoengineered endothelial cells onto blood-contacting surfaces

Canjuga, Denis; Hansen, Caroline E.; Halbrügge, Franziska; Hann, Ludmilla; Weiß, Sarina; Schlensak, Christian; Wendel, Hans-Peter; Avci‐Adali, Meltem

doi:10.1016/j.bioadv.2022.212824

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…Nevertheless, cellular adhesion and growth remain limited, leading to undesirable clinical events, such as neointimal hyperplasia or acute thrombosis. Therefore, several strategies, such as modification of the surface, have been explored to promote in situ endothelialization [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study

Avci‐Adali¹,

Grözinger²,

Cabane³

et al. 2023

Bioengineering

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Polytetrafluoroethylene (PTFE) is a commonly used biomaterial for the manufacturing of vascular grafts and several strategies, such as coatings, have been explored to improve the hemocompatibility of small-diameter prostheses. In this study, the hemocompatibility properties of novel stent grafts covered with electrospun PTFE (LimFlow Gen-1 and LimFlow Gen-2) were compared with uncoated and heparin-coated PTFE grafts (Gore Viabahn®) using fresh human blood in a Chandler closed-loop system. After 60 min of incubation, the blood samples were examined hematologically and activation of coagulation, platelets, and the complement system were analyzed. In addition, the adsorbed fibrinogen on the stent grafts was measured and the thrombogenicity was assessed by SEM. Significantly lower adsorption of fibrinogen was measured on the surface of heparin-coated Viabahn than on the surface of the uncoated Viabahn. Furthermore, LimFlow Gen-1 stent grafts showed lower fibrinogen adsorption than the uncoated Viabahn®, and the LimFlow Gen-2 stent grafts showed comparable fibrinogen adsorption as the heparin-coated Viabahn®. SEM analysis revealed no sign of thrombus formation on any of the stent surfaces. LimFlow Gen-2 stent grafts covered with electrospun PTFE exhibited bioactive characteristics and revealed improved hemocompatibility in terms of reduced adhesion of fibrinogen, activation of platelets, and coagulation (assessed by β-TG and TAT levels) similar to heparin-coated ePTFE prostheses. Thus, this study demonstrated improved hemocompatibility of electrospun PTFE. The next step is to conduct in vivo studies to confirm whether electrospinning-induced changes to the PTFE surface can reduce the risk of thrombus formation and provide clinical benefits.

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Section: Introductionmentioning

confidence: 99%

Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study

Avci‐Adali¹,

Grözinger²,

Cabane³

et al. 2023

Bioengineering

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“…Among them, hollow fber membrane contactors (HFMCs) are commonly employed in medical, pharmaceutical, biological, and environmental applications. Extracorporeal membrane oxygenation (e.g., biohybrid oxygenators [6][7][8], extracorporeal CO2 removal (ECCO2R) [9,10], arterio-venous CO 2 removal (AVCO2R) and venovenous CO 2 removal (VVCO2R) devices [11][12][13]), hemodialysis [14,15], tissue culturing [16], membrane bioreactors used for wastewater treatment [17][18][19][20], air purifcation (e.g., fue gas absorbing and CO 2 capturing [21][22][23][24][25][26][27]), and reverse osmosis applied in, e.g., water purifcation [28] and concentration process [29] are various examples where HFMCs are employed as one of their major components. In several applications, such as AVCO2R and VVCO2R, aqueous CO 2 removal is considered the signifcant performance objective of HFMCs [30].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Sweeping Media and Temperature on Aqueous CO2 Removal Using Hollow Fiber Membrane Contactor (HFMC): An Experimental Determination

Tabesh

Gholami

Marefat

2023

International Journal of Chemical Engineering

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Transport phenomena through hollow fiber membrane contactors (HFMCs) indicate the exchange of a component between the two phases, inside and outside of hollow fibers. In this research, we designed and fabricated lab-made HFMCs to assess the difference between water and air as sweeping media for CO2 exchange. The effects of flow rates and temperature ratios on aqueous CO2 absorption were investigated accordingly. A semiclosed circuit incorporating our fabricated HFMCs was set up to regulate the operating parameters and evaluate the aqueous CO2 concentration using an initiative pH-based method. The results of our experiments remarkably reveal that air tends to remove aqueous CO2 more than water when aqueous CO2 concentration is higher than 3.53 × 10−6 mlCO2/l. However, water would surpass air in lower concentrations. Nevertheless, tripling the flow rate of sweeping media from 500 to 1500 ml/min shifts up this cutoff point 50 times to around 1.66 × 10−4 mlCO2/l. The experiments performed at three different temperature ratios of 22 : 22, 44 : 12, and 22 : 12°C (CO2-rich liquid: sweeping medium) demonstrated that a higher temperature gradient deteriorates the CO2 absorption capacity of sweeping media. Nonetheless, temperature gradient becomes highly effective in aqueous CO2 concentrations lower than 1.57 × 10−6 CO2/l. The results of this research could be applied in performance optimization of aqueous CO2 absorbing HFMCs, even in sophisticated medical procedures such as arterio-venous and veno-venous CO2 removal systems where both water and air could be used as blood’s CO2 sweeping media.

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Programmable living materials joint special issue editorial

Zhong,

Sankaran

2023

Biomaterials Advances

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Improving hemocompatibility of artificial lungs by click conjugation of glycoengineered endothelial cells onto blood-contacting surfaces

Cited by 5 publications

References 35 publications

Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study

Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study

The Effect of Sweeping Media and Temperature on Aqueous CO2 Removal Using Hollow Fiber Membrane Contactor (HFMC): An Experimental Determination

Programmable living materials joint special issue editorial

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