Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation

Ma, Yuhao; To, Daniel; Zeng, Jie; Shoute, Lian C. T.; Wu, Meng; Babiuk, Shawn; Zhuo, Ran; Charlton, Carmen; Kanji, Jamil N; Babiuk, Lorne; Chen, Jie

doi:10.1016/j.biosx.2022.100176

Cited by 2 publications

(6 citation statements)

References 18 publications

(15 reference statements)

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“…The underlying principle of nano-enabled detection of emerging infectious diseases is to fabricate an appropriate nano-system to immobilize and detect target bio-active molecules with characteristic functional structures. Various nanobiosensors have been devised based on dual responsive photoplasmonic, [68][69][70][71][72] field-effect-transistor (FET) and electrochemical technologies 73 and guided gene recognition 74 that have enabled clinical-grade testing of COVID-19. Further integration with machine learning and the internet-of-things (IoT) can aid in real-time diagnosis and decision-making.…”

Section: Bionanotechnology In Emerging Infectious Diseasesmentioning

confidence: 99%

Bionanotechnology and bioMEMS (BNM): state-of-the-art applications, opportunities, and challenges

Borenstein,

Cummins,

Dutta

et al. 2023

Lab Chip

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Section: Bionanotechnology In Emerging Infectious Diseasesmentioning

confidence: 99%

Bionanotechnology and bioMEMS (BNM): state-of-the-art applications, opportunities, and challenges

Borenstein,

Cummins,

Dutta

et al. 2023

Lab Chip

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“…SARS-CoV-2 Spike protein was provided by Canadian Food Inspection Agency, in Dr. Shawn Babiuk's lab, Winnipeg, MB R3E 3M4, Canada. The preparation, purification, and functional evaluation of SARS-CoV-2 spike protein has been described in detail in an earlier publication [43,72,73]. The anti-SARS-CoV-2 antibody positive and negative serum samples were obtained from the lab of Drs.…”

Section: Sars-cov-2 Spike Protein and Serum Samplesmentioning

confidence: 99%

“…Sirus T2 Tabletop Reactive Ion Etcher was from Trion Technology, Clearwater, FL, USA was used plasma cleaning of the chips. Custom-built electrical contact pad with a connector adapter used for the Electrochemical Impedance Spectroscopy (EIS) measurements of the Au-IDA chip sensors has been described earlier [43,72,73].…”

Section: Instruments Electrical Contact Pad and Pdms Maskmentioning

confidence: 99%

“…The procedure adapted for the fabrication of gold IDA Chip on borofloat glass wafers using standard lift-off microfabrication technique has been described earlier [43,72,73]. Each Au-IDA chip has eight IDAs per chip which consists of 500 interdigitated comb electrodes arranged in an area of 3 mm × 3 mm.…”

Section: Ida Fabrication and Functionalizationmentioning

confidence: 99%

“…Immobilization of SARS-CoV-2 spike proteins on the baseline drift-stabilized MHAcoated Au-IDAs (MHA-Au-IDA) were carried out by using a PDMS mask which has eight matching square holes to form wells around the eight Au-IDAs on the chip and this allows MHA-Au-IDAs in the wells to be incubated with different solutions without intermixing [43,72,73]. The surface carboxylic acid group of the MHA-coated Au-IDA was activated by incubating for ~15 min in 50 µL aliquot of freshly prepared 73 mM EDC and 243 mM NHS solution in 10 mM MES buffer at pH 5.5.…”

Section: Spike Protein Immobilizationmentioning

confidence: 99%

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Faradaic Impedimetric Immunosensor for Label-Free Point-of-Care Detection of COVID-19 Antibodies Using Gold-Interdigitated Electrode Array

Shoute,

Charlton,

Kanji

et al. 2023

Biosensors

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Label-free electrochemical biosensors have many desirable characteristics in terms of miniaturization, scalability, digitization, and other attributes associated with point-of-care (POC) applications. In the era of COVID-19 and pandemic preparedness, further development of such biosensors will be immensely beneficial for rapid testing and disease management. Label-free electrochemical biosensors often employ [Fe(CN)6]−3/4 redox probes to detect low-concentration target analytes as they dramatically enhance sensitivity. However, such Faradaic-based sensors are reported to experience baseline signal drift, which compromises the performance of these devices. Here, we describe the use of a mecaptohexanoic (MHA) self-assembled monolayer (SAM) modified Au-interdigitated electrode arrays (IDA) to investigate the origin of the baseline signal drift, developed a protocol to resolve the issue, and presented insights into the underlying mechanism on the working of label-free electrochemical biosensors. Using this protocol, we demonstrate the application of MHA SAM-modified Au-IDA for POC analysis of human serum samples. We describe the use of a label-free electrochemical biosensor based on covalently conjugated SARS-CoV-2 spike protein for POC detection of COVID-19 antibodies. The test requires a short incubation time (10 min), and has a sensitivity of 35.4/decade (35.4%/10 ng mL−1) and LOD of 21 ng/mL. Negligible cross reactivity to seasonal human coronavirus or other endogenous antibodies was observed. Our studies also show that Faradaic biosensors are ~17 times more sensitive than non-Faradaic biosensors. We believe the work presented here contributes to the fundamental understanding of the underlying mechanisms of baseline signal drift and will be applicable to future development of electrochemical biosensors for POC applications.

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Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation

Cited by 2 publications

References 18 publications

Bionanotechnology and bioMEMS (BNM): state-of-the-art applications, opportunities, and challenges

Bionanotechnology and bioMEMS (BNM): state-of-the-art applications, opportunities, and challenges

Faradaic Impedimetric Immunosensor for Label-Free Point-of-Care Detection of COVID-19 Antibodies Using Gold-Interdigitated Electrode Array

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