Improving Infection Efficiency of Agrobacterium to Immature Cotyledon Explants of Japanese Apricot (Prunus mume) by Sonication Treatment

Gao–Takai, Mei; Tao, Ryutaro

doi:10.2503/jjshs1.ch-085

Cited by 4 publications

(2 citation statements)

References 38 publications

(45 reference statements)

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“…But, the transformation efficiency was low, and there was abnormal development of somatic embryos. Gao-Takai and Tao [40] improved the transformation efficiency of Japanese apricot by sonication treatment of immature cotyledons, and the efficiency was evaluated by transient GFP expression and frequency of somatic embryogenesis.…”

Section: Prunus Domestica Lmentioning

confidence: 99%

Can Prunus serotina be Genetically Engineered for Reproductive Sterility and Insect Pest Resistance?

Wang

Pijut

2014

Springer Science Reviews

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Black cherry (Prunus serotina) is a valuable hardwood timber species, and its value highly depends on the wood quality which is often threatened by insect pests. Transgenic black cherry plants that are more resistant to cambial-mining insects may reduce the occurrence of gummosis and have great economic benefits to landowners and the forest products industries utilizing black cherry lumber and logs. In this review, general information about black cherry and the problem of gummosis are introduced. The various strategies for gene containment, the possibility of using cyanogenesis to enhance host resistance, and the current status of micropropagation, adventitious shoot regeneration, rooting, and Agrobacterium-mediated transformation in P. serotina and several other Prunus species are briefly discussed. Keywords Agrobacterium Á Cyanogenesis Á Flowering Á Gummosis Á In vitro culture Á Prunus Á Reproductive sterility Á Transgene containment Black Cherry Black cherry (Prunus serotina Ehrh.), also known as wild black cherry, rum cherry, and mountain black cherry, is the only member in the genus Prunus that is of commercial importance as a timber species [96]. It is native to North America and is widely distributed throughout the eastern United States [28]. There are five varieties that are usually recognized based on the height of the tree and the thickness of the leaves: (1) P. serotina var. eximia (Small) Little; (2) P. serotina var. rufula (Woot. & Standl.) McVaugh; (3) P. serotina var. virens (Woot. & Standl.) McVaugh; (4) P. serotina var. salicifolia (Kunth) Koehne; and (5) P. serotina var. serotina. Among them, P. serotina var. serotina is the most common and widespread variety in the eastern United States and Canada [126]. Black cherry is one of the most valuable hardwoods for cabinets, furniture, veneer, architectural millwork, and musical instruments [93]. However, large and high-quality trees suited for commercial use (belonging to var. serotina) are only found in a restricted area on the Allegheny Plateau of Pennsylvania, New York, and West Virginia [57, 92]. Black cherry (subgenus Padus) is a deciduous and monoecious tree with moderate size. The leaves are 5-13cm in length and elliptical with serrated leaf margins. Its elongated racemes are small and have numerous white, perfect flowers in a leafy shoot, and its fruits are fleshy [14]. The fruits are acyanogenic throughout the developmental stages as they lack the catabolic enzymes, whereas the seeds become highly cyanogenic during the process of maturation with the accumulation of both the catabolic enzymes and cyanogenic glycosides [166]. Black cherry grows best in areas that are cool, moist, and temperate [41]. Black cherry serves as a host to a large number of pathogens. It is susceptible to soil-borne pathogens that are Endorsed by Paula M. Pijut.

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Section: Prunus Domestica Lmentioning

confidence: 99%

Can Prunus serotina be Genetically Engineered for Reproductive Sterility and Insect Pest Resistance?

Wang

Pijut

2014

Springer Science Reviews

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“…Agrobacterium tumefaciens-mediated transformation has been developed in several fruit species of Prunus, including Plum (P. domestica) 14 , Japanese Apricot (P. mume) 15 , Chinese Plum (P. salicina) 16 and Apricot (P. armeniaca) 17 . A. tumefaciens-mediated transformation has also been used to transfer visible marker genes encoding green fluorescent protein (GFP) or β-glucuronidase (GUS) to peach 5,18,19 .…”

mentioning

confidence: 99%

Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

Lai

Zhao

et al. 2020

Sci Rep

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Peach is an economically import fruit crop worldwide, and serves as a model species of the Rosaceae family as well. However, peach functional genomics studies are severely hampered due to its recalcitrance to regeneration and stable transformation. Here, we report a fast and efficient Agrobacterium rhizogenes-mediated transformation system in peach. Various explants, including leaf, hypocotyl and shoot, were all able to induce transgenic hairy roots, with a transformation efficiency of over 50% for hypocotyl. Composite plants were generated by infecting shoots with A. rhizogenes to induce transgenic adventitious hairy roots. The composite plant system was successfully used to validate function of an anthocyanin-related regulatory gene PpMYB10.1 in transgenic hairy roots, and two downstream genes, PpUFGT and PpGST, were strongly activated. Our stable and reproductive A. rhizogenes-mediated transformation system provides an avenue for gene function assay, genetic engineering, and investigation of root-rhizosphere microorganism interaction in peach. open Scientific RepoRtS | (2020) 10:2836 | https://doi.

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Effect of explant type and growth regulators on in vitro regeneration of apricot (Prunus armeniaca L.) Al-Amar rootstock

Elmoreigi,

El-Maaty,

Hassanen

et al. 2024

Plant Cell Tiss Organ Cult

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Apricot is a highly recalcitrant species for shoot regeneration in addition the plant regeneration capacity is strongly genotype – dependent. Thus, this study aimed to establish in vitro regeneration of the Egyptian Al-Amar apricot rootstock. Two explant types (Cotyledon and hypocotyl) were cultured on woody plant medium (WPM) supplemented with three concentrations of thidiazuron (TDZ) in combination with eight concentration of indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA). The cotyledons showed direct somatic organogenesis as the explants formed buds directly on regeneration media. The highest percentage of cotyledons producing buds was 78.00% when cultured on WPM containing 13.62 µM TDZ and 2.46 µM IBA. In contrast hypocotyls expressed indirect somatic organogenesis, as the explants produced callus first before bud formation. The highest percentage was 96.87% in hypocotyls cultured on WPM fortified with 15.89 µM TDZ and 1.61 µM NAA. The shoot proliferation was achieved when buds from cotyledons and hypocotyls were cultured on Soot Regeneration Medium (SRM) supplemented with 8.87 µM 6-Benzylaminopurine (BAP) and 0.54 µM NAA. The highest shoot formation with an average of 6.4 shoots per explant was obtained from hypocotyls, while an average of 2.0 shoots per explants was achieved from cotyledons. The regenerated shoots were rooted on WPM including 9.80 µM IBA. The results revealed that the regeneration of Al-Amar rootstock was more successful through hypocotyls and could therefore facilitate its genetic manipulation.

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Improving Infection Efficiency of Agrobacterium to Immature Cotyledon Explants of Japanese Apricot (Prunus mume) by Sonication Treatment

Cited by 4 publications

References 38 publications

Can Prunus serotina be Genetically Engineered for Reproductive Sterility and Insect Pest Resistance?

Can Prunus serotina be Genetically Engineered for Reproductive Sterility and Insect Pest Resistance?

Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

Effect of explant type and growth regulators on in vitro regeneration of apricot (Prunus armeniaca L.) Al-Amar rootstock

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