Improving Lactate Analysis with the YSI 2300 Gl: Hemolyzing Blood Samples Makes Results Comparable with Those for Deproteinized Whole Blood

Abstract

To obviate the well-documented problem of hematocrit dependency of the Yellow Springs Instruments (YSI) whole-blood lactate analyzer, we modified the dilution buffer by including a lysing reagent. This makes the results comparable with those of methods performed with deproteinized whole-blood samples. No centrifugation step is needed, thus preserving the convenience of the YSI instrument for stat and field use. The modification works equally well on plasma samples. Lactate concentrations measured in nonhemolyzed whole blood are not comparable with results for hemolyzed whole blood or protein-precipitated whole blood. We therefore recommend to all users of YSI equipment to lyse the erythrocytes before lactate determinations.

The Yo-Yo IE2 Test

“…km·h -1 for 60 s for the untrained and trained subjects respectively, followed by stepwise 1. Spring Instruments, Ohio, USA), with a CV value of 5% for duplicate measurements (9). The remainder of the blood collected was immediately centrifuged and the plasma removed and stored at -20 ºC.…”

Performance enhancements and muscular adaptations of a 16‐week recreational football intervention for untrained women

“…Blood samples were obtained in 2 mL syringes with heparin. Within 10 s of sampling 100 mL of blood was hemolyzed in an ice-cold 100 mL Triton X-100 buffer solution, and was later analyzed for lactate and glucose using an YSI 2300 lactate analyser (Foxdal et al, 1992;Yellow Spring Instruments, Yellow Springs, Ohio, USA).…”

Effect of previous exhaustive exercise on metabolism and fatigue development during intense exercise in humans

“…Plasma ammonia (NH 3 ) was determined spectophotometrically (Hellsten et al, 1999). Another part of the blood sample (100 mL) was hemolyzed using a 1:1 dilution with a buffer solution (Yellow Spring Instruments, Yellow Springs, Ohio, USA) to which 20 g/L Triton X-100 was added (Foxdal et al, 1992), for analysis of glucose and lactate concentrations (model 23, Yellow Spring Instruments). The rest of the blood sample was immediately placed in ice-cold water until measured for pH (ABL 700, Radiometer, Copenhagen, Denmark).…”