Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field

Abstract

This study aimed to improve the magnetofection of MG-63 osteoblasts by integrating the use of a novel uniform magnetic field with low molecular weight polyethylenimine modified superparamagnetic iron oxide nanoparticles (PEI-SPIO-NPs). The excellent characteristics of PEI-SPIO-NPs such as size, zeta potential, the pDNA binding and protective ability were determined to be suitable for gene delivery. The novel uniform magnetic field enabled polyethylenimine-modified superparamagnetic iron oxide nanoparticles/pDNA complexes (PEI-SPIO-NPs/pDNA complexes) to rapidly and uniformly distribute on the surface of MG-63 cells, averting local transfection and decreasing disruption of the membrane caused by the centralization of positively charged PEI-SPIO-NPs, thereby increasing the effective coverage of magnetic gene carriers during transfection, and improving magnetofection efficiency. This innovative uniform magnetic field can be used to determine the optimal amount between PEI-SPIO-NPs and pDNA, as well as screen for the optimal formulation design of magnetic gene carrier under the homogenous conditions. Most importantly, the novel uniform magnetic field facilitates the transfection of PEI-SPIO-NPs/pDNA into osteoblasts, thereby providing a novel approach for the targeted delivery of therapeutic genes to osteosarcoma tissues as well as a reference for the treatment of other tumors.

Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

“…Cen and colleagues also showed that the transfection efficiency of PEI-ION/pDNA complexes into human osteosarcoma cells (MG-63) was significantly increased by the application of a uniform magnetic field (about four-fold increase) [ 392 ]. Moreover, they found that by changing from a non-uniform to a uniform magnetic field, the efficiency doubled to 42.1% [ 392 ]. In the same way, Vaca and colleagues studied the effect of MF time and the culture-to-magnet relative position on the transfection efficiency and cellular uptake [ 381 ].…”

Magnetic transfection with superparamagnetic chitosan-loaded IGFBP5nanoparticles and theirin vitrobiosafety

“…However, magnetic transfection was used in this study, which forced the transfection vector and the target cell to stay in contact. In addition, the oscillation induced by magnetic transfection on the target cell surface can cause the nanoparticle complex to move, which helps increase the transfection rate by mechanically stimulating endocytosis [ 30 , 46 , 47 ]. Other researchers have shown that magnetic transfection can effectively increase the transfection rate of gene vectors [ 47 , 48 ].…”

Estimating the Two Graph Dextran-Stearic Acid-Spermine Polymers Based on Iron Oxide Nanoparticles as Carrier for Gene Delivery

“…In aqueous environment, spermine residues in DSASP-NPs are localized outside the micelle formation due to its hydrophobicity property. Considering to cell membrane negatively charged, the positively charged complexes facilitate its cellular uptake [41].…”

“…The issue of stability in formation of polycations-based nanocomplexes with negative phosphate backbone charge of DNA is considered as a substantial requirement in gene delivery applications to achieve proper transfection efficiency. Preparation of various weight ratio of polycations to DNA is assumed as one of the most significant parameters to achieve this process [41,45]. So, a different mass ratio of DSASP was mixed with similar volumes of pDNA and then results were analyzed based on gel retardation assay.…”