The impact of changing meteorological conditions on crop quality has become a trending topic in current agriculture research. In this study, we analyzed the combined effects of both meteorological conditions and key stages on the nutritional quality of maize based on the data of field-staged sowing trials from 2018 to 2022. The results are as follows: (1) The key stage of meteorological conditions affecting the content of major nutritional qualities of maize is from 6 d before to 35 d after flowering. (2) The maximum temperature from 6 d before to 8 d after flowering, average temperature from 5 d before to 20 d after flowering, and minimum temperature from 9–20 d after flowering have significant positive effects on protein, fat, and essential amino acids, respectively; the daily difference in temperature from 9 to 35 d and 24 to 35 d after flowering have a significant negative effect on crude fiber and essential amino acids, respectively, and the daily difference in temperature from 24 to 35 d and the minimum temperature from 5 d before to 35 d after flowering have a significant effect on non-essential amino acids. (3) When the maximum temperature during the key stage of nutritional quality is 31.2 °C, the average temperatures are 24.9 °C and 22.4 °C, the minimum temperature is 18.9 °C, and the daily difference in temperature is 15.0 °C, the contents could reach the optimal values of 9.66% (protein), 4.80% (fat), 4.97% (crude fiber), 40.39 g·kg−1 (essential amino acids), and 58.96 g·kg−1 (non-essential amino acids), respectively. The findings provide a basis for adjusting the sowing period to improve the nutritional quality of maize in the context of climate change.