Improving osteoblasts cells proliferation via femtosecond laser surface modification of 3D-printed poly-ε-caprolactone scaffolds for bone tissue engineering applications

Daskalova, A.; Ostrowska, Barbara; Zhelyazkova, A.; Święszkowski, Wojciech; Trifonov, Anton; Declercq, Heidi; Nathala, Chandra Sekher; Szlązak, Karol; Łojkowski, Maciej; Husinsky, W.; Buchvarov, Ivan

doi:10.1007/s00339-018-1831-y

Cited by 9 publications

(11 citation statements)

References 49 publications

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“…This confirmed that the laser treated part of the samples surface created even better conditions for cells proliferation on the “bone mimic medium”. This further supports and develops previous research of the group [ 33 , 34 ]. The results obtained are of great importance in tissue engineering due to the requirement of a suitable microstructured matrix, which triggers the development of a large number of oriented cells for bone tissue repairing.…”

Section: Resultssupporting

confidence: 92%

“…Different reports have indicated that the surface properties drastically influence cell behaviors [ 33 , 34 , 35 , 47 , 56 , 57 , 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the antibacterial effect, by reducing cell toxicity, could be significantly improved by the incorporation of Ag nanoparticles into the composite films. As a continuation of our recent examinations on the processing of biopolymer thin chitosan films [ 33 , 34 , 35 , 36 , 37 ], we focused towards the creation of patterning designs and characterization of thin films of pure chitosan and its composite scaffolds from HAp/ZrO 2 for achievement of successful platforms for bone tissue repair and engineering characterized not only by high biocompatibility, biodegradability, and affinity to proteins, but also by good chemical and dimensional stability, mechanical strength, and toughness, which will improve the tissue engineering applications of the scaffolds.…”

Section: Introductionmentioning

confidence: 98%

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Femtosecond Laser Fabrication of Engineered Functional Surfaces Based on Biodegradable Polymer and Biopolymer/Ceramic Composite Thin Films

et al. 2019

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Surface functionalization introduced by precisely-defined surface structures depended on the surface texture and quality. Laser treatment is an advanced, non-contact technique for improving the biomaterials surface characteristics. In this study, femtosecond laser modification was applied to fabricate diverse structures on biodegradable polymer thin films and their ceramic blends. The influences of key laser processing parameters like laser energy and a number of applied laser pulses (N) over laser-treated surfaces were investigated. The modification of surface roughness was determined by atomic force microscopy (AFM). The surface roughness (Rrms) increased from approximately 0.5 to nearly 3 µm. The roughness changed with increasing laser energy and a number of applied laser pulses (N). The induced morphologies with different laser parameters were compared via Scanning electron microscopy (SEM) and confocal microscopy analysis. The chemical composition of exposed surfaces was examined by FTIR, X-ray photoelectron spectroscopy (XPS), and XRD analysis. This work illustrates the capacity of the laser microstructuring method for surface functionalization with possible applications in improvement of cellular attachment and orientation. Cells exhibited an extended shape along laser-modified surface zones compared to non-structured areas and demonstrated parallel alignment to the created structures. We examined laser-material interaction, microstructural outgrowth, and surface-treatment effect. By comparing the experimental results, it can be summarized that considerable processing quality can be obtained with femtosecond laser structuring.

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Section: Resultssupporting

confidence: 92%

“…Different reports have indicated that the surface properties drastically influence cell behaviors [ 33 , 34 , 35 , 47 , 56 , 57 , 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Femtosecond Laser Fabrication of Engineered Functional Surfaces Based on Biodegradable Polymer and Biopolymer/Ceramic Composite Thin Films

et al. 2019

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“…PCL-based 3D scaffolds, resembling a woodpile construct, were fabricated via extrusion 3D printing. The standard operating procedure was already described and established by Daskalova et al [24]. In brief, PCL pellets with M n = 45 kDa (Sigma-Aldrich, St. Louis, MO, USA) were melted at 70 • C in a cartridge unit and extruded through a 250 µm needle at a pressure of 5 bar and speed of deposition of 95 mm/min.…”

Section: Fabrication Of Polymeric Scaffoldsmentioning

confidence: 99%

Investigating Potential Effects of Ultra-Short Laser-Textured Porous Poly-ε-Caprolactone Scaffolds on Bacterial Adhesion and Bone Cell Metabolism

et al. 2022

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Developing antimicrobial surfaces that combat implant-associated infections while promoting host cell response is a key strategy for improving current therapies for orthopaedic injuries. In this paper, we present the application of ultra-short laser irradiation for patterning the surface of a 3D biodegradable synthetic polymer in order to affect the adhesion and proliferation of bone cells and reject bacterial cells. The surfaces of 3D-printed polycaprolactone (PCL) scaffolds were processed with a femtosecond laser (λ = 800 nm; τ = 130 fs) for the production of patterns resembling microchannels or microprotrusions. MG63 osteoblastic cells, as well as S. aureus and E. coli, were cultured on fs-laser-treated samples. Their attachment, proliferation, and metabolic activity were monitored via colorimetric assays and scanning electron microscopy. The microchannels improved the wettability, stimulating the attachment, spreading, and proliferation of osteoblastic cells. The same topography induced cell-pattern orientation and promoted the expression of alkaline phosphatase in cells growing in an osteogenic medium. The microchannels exerted an inhibitory effect on S. aureus as after 48 h cells appeared shrunk and disrupted. In comparison, E. coli formed an abundant biofilm over both the laser-treated and control samples; however, the film was dense and adhesive on the control PCL but unattached over the microchannels.

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“…and geometry according to clinical demands. 38,39 Unlike simple biomaterials, composite materials, such as pore-wall reinforced bioceramic scaffolds, could potentially meet the diverse requirements of large bone defect repair. 40,41 However, traditional methods of pore-wall modification, such as plasma-spraying and foam-hanging, have had difficulty achieving homogeneous and dense modified coating layers.…”

Section: F I G U R Ementioning

confidence: 99%

Modification of pore‐wall in direct ink writing wollastonite scaffolds favorable for tuning biodegradation and mechanical stability and enhancing osteogenic capability

Qiu

Wang

et al. 2020

The FASEB Journal

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Surface chemistry and mechanical stability determine the osteogenic capability of bone implants. The development of high‐strength bioactive scaffolds for in‐situ repair of large bone defects is challenging because of the lack of satisfying biomaterials. In this study, highly bioactive Ca‐silicate (CSi) bioceramic scaffolds were fabricated by additive manufacturing and then modified for pore‐wall reinforcement. Pure CSi scaffolds were fabricated using a direct ink writing technique, and the pore‐wall was modified with 0%, 6%, or 10% Mg‐doped CSi slurry (CSi, CSi‐Mg6, or CSi‐Mg10) through electrostatic interaction. Modified CSi@CSi‐Mg6 and CSi@CSi‐Mg10 scaffolds with over 60% porosity demonstrated an appreciable compressive strength beyond 20 MPa, which was ~2‐fold higher than that of pure CSi scaffolds. CSi‐Mg6 and CSi‐Mg10 coating layers were specifically favorable for retarding bio‐dissolution and mechanical decay of scaffolds in vitro. In‐vivo investigation of critical‐size femoral bone defects repair revealed that CSi@CSi‐Mg6 and CSi@CSi‐Mg10 scaffolds displayed limited biodegradation, accelerated new bone ingrowth (4‐12 weeks), and elicited a suitable mechanical response. In contrast, CSi scaffolds exhibited fast biodegradation and retarded new bone regeneration after 8 weeks. Thus, tailoring of the chemical composition of pore‐wall struts of CSi scaffolds is beneficial for enhancing the biomechanical properties and bone repair efficacy.

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Improving osteoblasts cells proliferation via femtosecond laser surface modification of 3D-printed poly-ε-caprolactone scaffolds for bone tissue engineering applications

Cited by 9 publications

References 49 publications

Femtosecond Laser Fabrication of Engineered Functional Surfaces Based on Biodegradable Polymer and Biopolymer/Ceramic Composite Thin Films

Femtosecond Laser Fabrication of Engineered Functional Surfaces Based on Biodegradable Polymer and Biopolymer/Ceramic Composite Thin Films

Investigating Potential Effects of Ultra-Short Laser-Textured Porous Poly-ε-Caprolactone Scaffolds on Bacterial Adhesion and Bone Cell Metabolism

Modification of pore‐wall in direct ink writing wollastonite scaffolds favorable for tuning biodegradation and mechanical stability and enhancing osteogenic capability

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