The article presents the results of field experiments on deepening the arable horizon of gray forest medium loamy soil to increase its fertility through applying organic fertilizer under PY-3-35 layer plow to a depth of 2527 cm. Low mineralization of organic fertilizer during layer cultivation under oxygen deficiency conditions contributed to accumulation of humus in soil by 6.6 t/ha more than during conventional plowing, and by 7.5 t/ha - than during disking. It also improved water-physical and biological properties of soil: number of water-resistant aggregates increased by 4.6 and 5.3 %, soil density lowered by 0.03 and 0.04 g/cm3, number of earthworms increased by 3...6 individuals, expanded reproduction of fertility was provided, productivity of arable land increased by 7.0 and 6.7 % and crop quality increased compared to conventional plowing and disking, respectively. Deep manure incorporation extended life of organic fertilizer up to 5 years, while after conventional plowing and disking this process lasted only 2...3 years. It is not economically and environmentally beneficial, since frequent application requires a lot of fuel, and a large amount of harmful chemical compounds contained in combustion products is dumped into the environment.