Improving Photosynthetic Capacity, Alleviating Photosynthetic Inhibition and Oxidative Stress Under Low Temperature Stress With Exogenous Hydrogen Sulfide in Blueberry Seedlings

Abstract

In this study, we investigated the mechanism of photosynthesis and physiological function of blueberry leaves under low temperature stress (4-6°C) by exogenous hydrogen sulfide (H 2 S) by spraying leaves with 0.5 mmol·L -1 NaHS (H 2 S donor) and 200 mmol·L -1 hypotaurine (Hypotaurine, H 2 S scavenger). The results showed that chlorophyll and carotenoid content in blueberry leaves decreased under low temperature stress, and the photochemical activities of photosystem II (PSII) and photosystem I (PSI) were also inhibited. Low temperature stress can reduce photosynthetic carbon assimilation capacity by inhibiting stomatal conductance (G s ) of blueberry leaves, and non-stomatal factors also play a limiting role at the 5 th day of low temperature stress. Low temperature stress leads to the accumulation of Pro and H 2 O 2 in blueberry leaves and increases membrane peroxidation. Spraying leaves with NaHS, a donor of exogenous H 2 S, could alleviate the degradation of chlorophyll and carotenoids in blueberry leaves caused by low temperature and reduce the photoinhibition of PSII and PSI. The main reason for the enhancement of photochemical activity of PSII was that exogenous H 2 S promoted the electron transfer from Q A to Q B on PSII acceptor side under low temperature stress. In addition, it promoted the accumulation of osmotic regulator proline under low temperature stress and significantly alleviated membrane peroxidation. H 2 S scavengers (Hypotaurine) aggravated photoinhibition and the degree of oxidative damage under low temperature stress. Improving photosynthetic capacity as well as alleviating photosynthetic inhibition and oxidative stress with exogenous H 2 S is possible in blueberry seedlings under low temperature stress.

Antioxidative response and photosynthetic regulatory mechanisms in common fig leaves after short‐term chilling stress

“…Maximum quantum yield of primary ( φ P0 ) was considered as good indicator of PSII photodamage since its decrease is associated to the turnover of D1 protein (Desotgiu et al, 2012; Li & Zhang, 2016; Ohira, Morita, Suh, Jung, & Yamamoto, 2005). Although there was only a slight degradation of D1 protein in both fig varieties, it might cause the inhibition of electron flow from Q A to Q B (Chen et al, 2012; Tang et al, 2020). Chilling stress inhibited quantum efficiency for the electron transport, expressed as φ E0 or ET 0 /ABS (Yusuf et al, 2010) in both fig varieties Zamorčica and Green matalon (Figure 2).…”

Unraveling the mechanisms controlling Cd accumulation and Cd‐tolerance in Brachiaria decumbens and Panicum maximum under summer and winter weather conditions

“…The efficient functioning of the antioxidative system, mainly the AsA‐GSH cycle, is very important to protect the photosynthetic apparatus against deleterious Cd effects resulting from increased ROS production (e.g. chlorophyll degradation; Parmar et al 2013, Bora et al 2020, Tang et al 2020). Oxidative stress can cause protein oxidation and lipid peroxidation, leading to disruption of thylakoid membranes and alterations in the structure and amount of grana, which interrupts the reactions in the photosystem II (PSII) and changes the electrons flow between PSII and PSI (Parmar et al 2013, Andresen et al 2020).…”

“…It is known that the oxidative stress caused by increased levels of reactive oxygen species (ROS) is higher at lower temperatures (Tang et al 2020), as observed in several forage grasses (Loka et al 2019), and that oxidative stress should be the primary result of Cd toxicity in plants (Loix et al 2017). Oxidative stress can cause protein oxidation and lipid peroxidation, which cause the rupture of the plasma membranes and change the structure and numbers of chloroplasts in leaf cells, besides to changes of the water relations and consequently the stomatal conductance and transpiration (Parmar et al 2013), as observed in P. maximum exposed to Cd (Rabêlo et al 2018d).…”

Crosstalk between Hydrogen Sulfide and Other Signal Molecules Regulates Plant Growth and Development

“…In tall fescue, 500 μM H 2 S increased photochemical efficiency and antioxidant enzyme activities while reducing the levels of H 2 O 2 and MDA under low-light stress conditions [ 48 ]. In blueberry seedlings, exogenous 500 μM H 2 S alleviated low temperature stress by maintaining the content of chlorophyll, carotenoids, and the osmotic regulator proline and by reducing photosynthetic inhibition and membrane peroxidation [ 49 ].…”