Improving phytase production in Pichia pastoris fermentations through de‐repression and methanol induction optimization
Carlos H. Luna‐Flores,
Yilun Weng,
Alexander Wang
et al.
Abstract:Pichia pastoris (Komagataella phaffii) is a fast‐growing methylotrophic yeast with the ability to assimilate several carbon sources such as methanol, glucose, or glycerol. It has been shown to have outstanding secretion capability with a variety of heterologous proteins. In previous studies, we engineered P. pastoris to co‐express Escherichia coli AppA phytase and the HAC1 transcriptional activator using a bidirectional promoter. Phytase production was characterized in shake flasks and did not reflect industri… Show more
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