Improving plant transformation using Agrobacterium tumefaciens

Neto, L V Ribeiro; Oliveira, Ana Paula de; Lourenço, Miriam Vergínia; Bertoni, Bianca Waléria; França, Suzelei de Castro; Rosa-Santos, Thiago Mateus; Zingaretti, Sônia Marli

doi:10.4238/2015.june.18.13

Cited by 2 publications

(1 citation statement)

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“…In the last decades, several methods have been developed and changes incorporated in the transformation process via A. tumefaciens, such as the addition of acetosyringone and changes in the periods of co-cultivation for increased efficiency (Mayo et al 2006;Ribeiro-Neto et al 2015). Studies on plant models such as Arabidopsis thaliana and Nicotiana tabacum, which have useful characteristics, such as short generation time and large numbers of progeny (Sparkes et al 2006;Xiao et al 2015), help in the development of improved plant transformation methods for studying genes of interest (Begcy et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Research Article Efficient method for Agrobacterium-mediated genetic transformation of tobacco nodal segments

Silva¹,

Coppede²,

Silva³

et al. 2018

Genet. Mol. Res.

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Plant transformation is a widely used procedure for obtaining transgenic plants and to develop plant models to understand gene function. Plant models such as Nicotiana tabacum are widely used for understanding gene responses to external influences. An important tool in such studies is genetic transformation through infection with Agrobacterium tumefaciens. However, this transformation is often inefficient. Consequently, development and optimization of techniques to promote high rates of seedling regeneration of transgenic tobacco is imperative. The methods tested for infection of tobacco explants consisted of injecting 10 μl of the bacterial culture directly into anodal segment using an insulin syringe (1 mL); bacterial co-cultivation with nodal segments and micro-sectioned leaf disks. Infection through punctures made with a syringe in nodal segments of tobacco and no co-cultivation period was the most efficient in the regeneration process and in obtaining genetically transformed plants, with 88 and 75% success rates, respectively. We obtained an increase of 50% in the transformation rates when compared to previous studies using N. tabacum.

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