Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications<i>via</i>saponification and heat treatment processes

Suntornnond, Ratima; Ng, Wei Long; Huang, Xi; Yeow, Chuen Herh Ethan; Yeong, Wai Yee

doi:10.1039/d2tb00442a

Cited by 50 publications

(31 citation statements)

References 70 publications

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“…The control of the physical properties of the polymer was achieved without degrading the functional groups of the polymer, unlike hydrolysis. [ 26 ] This was confirmed by NMR spectroscopy (Figure 3C), and the cell spreading morphology of the 10% 20 h hydrogel also indicated that the cell adhesion motifs (i.e., the RGD motif) were not eliminated after sonication (Figure 6C). As higher concentration inks become printable after sonochemical degradation, hydrogels with improved physical properties could be produced by inkjet printing (Figure 5).…”

Section: Discussionmentioning

confidence: 72%

“…[ 25,26 ] However, this chemical treatment inevitably involves the loss of the methacryloyl group, which is the crosslinking site, resulting in a mechanically weaker hydrogel. [ 26 ]…”

Section: Introductionmentioning

confidence: 99%

“…Chemical hydrolysis with a strong base (i.e., sodium hydroxide [NaOH]) has been used to degrade the GelMA polymer to achieve the optimal jetting behavior of highconcentration bioinks. [25,26] However, this chemical treatment inevitably involves the loss of the methacryloyl group, which is the crosslinking site, resulting in a mechanically weaker hydrogel. [26] In this work, we describe the sonochemical degradation of biopolymers as a means to control viscoelasticity for drop-ondemand inkjet bioprinting.…”

Section: Introductionmentioning

confidence: 99%

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Sonochemical Degradation of Gelatin Methacryloyl to Control Viscoelasticity for Inkjet Bioprinting

Lee

Park

Tuladhar³

et al. 2023

Macromolecular Bioscience

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Inkjet printing enables the mimicry of the microenvironment of natural complex tissues by patterning cells and hydrogels at a high resolution. However, the polymer content of an inkjet‐printable bioink is limited as it leads to strong viscoelasticity in the inkjet nozzle. Here it is demonstrated that sonochemical treatment controls the viscoelasticity of a gelatin methacryloyl (GelMA) based bioink by shortening the length of polymer chains without causing chemical destruction of the methacryloyl groups. The rheological properties of treated GelMA inks are evaluated by a piezo‐axial vibrator over a wide range of frequencies between 10 and 10 000 Hz. This approach enables to effectively increase the maximum printable polymer concentration from 3% to 10%. Then it is studied how the sonochemical treatment effectively controls the microstructure and mechanical properties of GelMA hydrogel constructs after crosslinking while maintaining its fluid properties within the printable range. The control of mechanical properties of GelMA hydrogels can lead fibroblasts more spreading on the hydrogels. A 3D cell‐laden multilayered hydrogel constructs containing layers with different physical properties is fabrictated by using high‐resolution inkjet printing. The sonochemical treatment delivers a new path to inkjet bioprinting to build microarchitectures with various physical properties by expanding the range of applicable bioinks.

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Section: Discussionmentioning

confidence: 72%

“…[ 25,26 ] However, this chemical treatment inevitably involves the loss of the methacryloyl group, which is the crosslinking site, resulting in a mechanically weaker hydrogel. [ 26 ]…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sonochemical Degradation of Gelatin Methacryloyl to Control Viscoelasticity for Inkjet Bioprinting

Lee

Park

Tuladhar³

et al. 2023

Macromolecular Bioscience

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“…Different systems based on solenoid valves and pressurized material-suppling tubings and on acoustic- and electro-assisted devices have been the alternative to drop-on-demand (DOD) GelMA-based bioprinting; nevertheless, larger drops with the concomitant loss in resolution are unavoidable. A second approach has been to modify further the chemical and structural nature , of GelMA, allowing befitted rheological properties at the expense of losing bioactivity and mechanical resistance post cross-linking.…”

Section: Introductionmentioning

confidence: 99%

A Psychrophilic GelMA: Breaking Technical and Immunological Barriers for Multimaterial High-Resolution 3D Bioprinting

et al. 2022

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The increasing demand for tissue replacement has encouraged scientists worldwide to focus on developing new biofabrication technologies. Multimaterials/cells printed with stringent resolutions are necessary to address the high complexity of tissues. Advanced inkjet 3D printing can use multimaterials and attain high resolution and complexity of printed structures. However, a decisive yet limiting aspect of translational 3D bioprinting is selecting the befitting material to be used as bioink; there is a complete lack of cytoactive bioinks with adequate rheological, mechanical, and reactive properties. This work strives to achieve the right balance between resolution and cell support through methacrylamide functionalization of a psychrophilic gelatin and new fluorosurfactants used to engineer a photo-cross-linkable and immunoevasive bioink. The syntonized parameters following optimal formulation conditions allow proficient printability in a PolyJet 3D printer comparable in resolution to a commercial synthetic ink (∼150 μm). The bioink formulation achieved the desired viability (∼80%) and proliferation of co-printed cells while demonstrating in vivo immune tolerance of printed structures. The practical usage of existing high-resolution 3D printing systems using a novel bioink is shown here, allowing 3D bioprinted structures with potentially unprecedented complexity.

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“…However, there are some challenges faced in translating this to widespread use. Successful production of in-vitro tissue models is dependent on two critical aspects–ability to carry out large-scale manufacturing of cells (growing cells in vast quantities within a homogeneous physical and chemical environment) ( Jordan et al, 2018 ; He et al, 2019 ; Chen et al, 2022 ) and advanced manufacturing platforms (highly-automated fabrication of in-vitro tissue models with high throughput rates and repeatability) ( Ozbolat and Hospodiuk, 2016 ; Ng et al, 2019 ; Zhuang et al, 2019 ; Ng et al, 2020a ; Ng et al, 2020b ; Li et al, 2020 ; Ng et al, 2022 ; Suntornnond et al, 2022 ).…”

mentioning

confidence: 99%

Editorial: Fabrication of in-vitro 3D human tissue models—From cell processing to advanced manufacturing

Naing

Suntornnond

et al. 2022

Front. Bioeng. Biotechnol.

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Over the years, the field of toxicology testing has pivoted from the use of animal models to 2D human cell cultures and finally the adaptation of in-vitro 3D human testing models. It is important that these in-vitro 3D human testing models predicts the human responses in an accurate and reliable manner (Nam et al., 2015;Ng and Yeong, 2019). This is largely driven by the significant discrepancies between adverse effects of chemicals in humans and animals (Lilienblum et al., 2008). The use of animal models has several caveats which include the differences in the absorption or distribution of the chemicals/substances; the way the substances are metabolized and the short duration of animal lifespan to accurately monitor disease development. Similarly, conventional 2D cell culture is unable to adequately recapitulate the in vivo cell-cell and cell-matrix interactions found in native three-dimensional (3D) tissues and it has been reported that numerous types of cells have expressed different phenotypes and genomic profiles in 2D versus 3D cell culture (Duval et al., 2017;Jensen and Teng, 2020).Hence, in-vitro 3D human tissue models would bring about the necessary complexity that may improve the reliability and accuracy of test outcomes. Some of the fabricated in-vitro 3D human tissue models for various testing applications include skin tissue models (

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Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applicationsviasaponification and heat treatment processes

Abstract: Material jetting bioprinting is a highly promising three-dimensional (3D) bioprinting technique that facilitates drop-on-demand (DOD) deposition of biomaterials and cells at pre-defined positions with high precision and resolution. A major...

Cited by 50 publications

References 70 publications

Sonochemical Degradation of Gelatin Methacryloyl to Control Viscoelasticity for Inkjet Bioprinting

Sonochemical Degradation of Gelatin Methacryloyl to Control Viscoelasticity for Inkjet Bioprinting

A Psychrophilic GelMA: Breaking Technical and Immunological Barriers for Multimaterial High-Resolution 3D Bioprinting

Editorial: Fabrication of in-vitro 3D human tissue models—From cell processing to advanced manufacturing

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