Improving qPCR methodology for detection of foaming bacteria by analysis of broad-spectrum primers and a highly specific probe for quantification of<i>Nocardia</i>spp. in activated sludge

Asvapathanagul, Pitiporn; Olson, Betty H.

doi:10.1111/jam.13311

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…Scientific literature reports hundreds of applications of conventional and innovative biological tools, as well as examples of investigations based on ecological criteria [9,[20][21][22][23][24][25][26][27]. Almost all the biological processes have been explored in terms of biomass composition (bacteria, protozoa, metazoan, and fungi) and activity: conventional activated sludge, attached biomass, membrane reactors, and treating either municipal [27][28][29][30][31][32][33][34] or industrial wastewater [35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Abbà

Collivignarelli

Manenti

et al. 2017

Journal of Chemistry

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A thermophilic aerobic membrane reactor (TAMR) treating high-strength COD liquid wastes was submitted to an integrated investigation, with the aim of characterizing the biomass and its rheological behaviour. These processes are still scarcely adopted, also because the knowledge of their biology as well as of the physical-chemical properties of the sludge needs to be improved. In this paper, samples of mixed liquor were taken from a TAMR and submitted to fluorescent in situ hybridization for the identification and quantification of main bacterial groups. Measurements were also targeted at flocs features, filamentous bacteria, and microfauna, in order to characterize the sludge. The studied rheological properties were selected as they influence significantly the performances of membrane bioreactors (MBR) and, in particular, of the TAMR systems that operate under thermophilic conditions (i.e., around 50 ∘ C) with high MLSS concentrations (up to 200 gTS L −1 ). The proper description of the rheological behaviour of sludge represents a useful and fundamental aspect that allows characterizing the hydrodynamics of sludge suspension devoted to the optimization of the related processes. Therefore, in this study, the effects on the sludge rheology produced by the biomass concentration, pH, temperature, and aeration were analysed.

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Section: Introductionmentioning

confidence: 99%

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Abbà

Collivignarelli

Manenti

et al. 2017

Journal of Chemistry

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“…The phylogenetic tree of the Pseudonocardia species was drawn with the Mega 5 software and is depicted in Figure 1. Marmur and Doty reported the DNA G+C content using thermal denaturation with Escherichia coli AS1.365 as the control (Asvapathanagul & Olson, 2017; Marmur & Doty, 1962). Based on the thermal renaturation method in a UV‐1206 spectrophotometer equipped with a TB‐85 thermo‐bath, De Ley et al performed a DNA–DNA hybridization test (Huss et al, 1983; Ley et al, 1970; Yassin et al, 1993).…”

Section: Molecular Methods For Pseudonocardia Identificationmentioning

confidence: 99%

Genus Pseudonocardia : What we know about its biological properties, abilities and current application in biotechnology

Riahi

Heidarieh

Bafghi

2021

J of Applied Microbiology

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The genus Pseudonocardia belongs to a group of actinomycetes, and is a member of the family Pseudonocardiacea. The members of this genus are aerobic, Gram-positive, non-motile bacteria that are commonly found in soil, plant, and environment. Although this genus has a low clinical significance, however it has an important role in biotechnology due to the production of secondary metabolites, some of which have anti-bacterial, anti-fungal, and anti-tumor effects. The use of phenotypic tests such as gelatinase activity, starch hydrolysis, catalase, and oxidase tests, as well as molecular methods, such as polymerase chain reaction (PCR), are necessary for Pseudonocardia identification at the genus and species levels.

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Impact of sludge bulking on receiving environment using quantitative microbial risk assessment (QMRA)-based management for full-scale wastewater treatment plants

Deepnarain

Nasr

Amoah

et al. 2020

Journal of Environmental Management

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Improving qPCR methodology for detection of foaming bacteria by analysis of broad-spectrum primers and a highly specific probe for quantification ofNocardiaspp. in activated sludge

Abstract: Degenerative primers are very useful in amplifying bacterial DNA across genera, but reduce the efficiency of qPCR reactions. Therefore, standards that address closely related background species must be used to obtain accurate qPCR results.

Cited by 4 publications

References 33 publications

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Genus Pseudonocardia : What we know about its biological properties, abilities and current application in biotechnology

Impact of sludge bulking on receiving environment using quantitative microbial risk assessment (QMRA)-based management for full-scale wastewater treatment plants

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