Improving rainbow trout (Oncorhynchus mykiss) growth by treatment with a fish (Paralichthys olivaceus) myostatin prodomain expressed in soluble forms in E. coli

Lee, Sang Beum; Kim, Yong Soo; Oh, Mi-Yong; Jeong, In-Hak; Seong, Ki-Baik; Jin, Hyung‐Joo

doi:10.1016/j.aquaculture.2010.02.027

Cited by 25 publications

(22 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, it was observed that MSTN b was hardly detected before hatching, and MSTN a was detected as early as 2 hpf, with two peaks at 4 hpf and 24 hpf, respectively, which suggested that at least two genes were controlled by different transregulatory factors, and they may ultimately had evolved different physiological roles. Some studies had speculated that in teleost MSTN a appeared to primarily inhibit muscle hyperplasia rather than hypertrophy, and that growth through muscle hypertrophy was only attained when the MSTN b expression was down-regulated (Lee et al, 2010;McPherron et al, 1997). There was no direct evidence to confirm whether MSTN a was inhibited or hypertrophied, but at least, we found that MSTN a was correlated with somitogenesis, while MSTN b was not in M. amblycephala.…”

Section: Genescontrasting

confidence: 79%

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Zeng

Liu

Wang

et al. 2014

Gene

View full text Add to dashboard Cite

Section: Genescontrasting

confidence: 79%

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Zeng

Liu

Wang

et al. 2014

Gene

View full text Add to dashboard Cite

“…An increase in mass has been reported for fish species capable of indeterminate growth (i.e. non-growth limited) following injection or submersion in a bath of a soluble form of the Acvr2b (Carpio et al, 2009) or myostatin pro-domain (Lee et al, 2010a); however, this research was limited to only larval and early juvenile life stages. In the research presented here, transgenic trout overexpressing a truncated acvr2b-2a (acvr2b ∆ ) were produced to investigate the involvement of Acvr2b signaling in the long-term regulation of muscle mass in a teleost species with indeterminate growth.…”

Section: Introductionmentioning

confidence: 99%

Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor

Phelps

Jaffe

Bradley

2013

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYThe activin type IIB receptor (Acvr2b) is the cell surface receptor for multiple transforming growth factor β (TGF-β) superfamily ligands, several of which regulate muscle growth in mammals. To investigate the role of the Acvr2b signaling pathway in the growth and development of skeletal muscle in teleost fish, transgenic rainbow trout (RBT; Oncorhynchus mykiss) expressing a truncated form of the acvr2b-2a (acvr2b ∆ ) in muscle tissue were produced. High levels of acvr2b ∆ expression were detected in the majority of P1 transgenic fish. Transgenic P1 trout developed enhanced, localized musculature in both the epaxial and hypaxial regions (dubbed 'six pack'). The F1 transgenic offspring did not exhibit localized muscle growth, but rather developed a uniform body morphology with greater girth, condition factor and increased muscle fiber hypertrophy. There was a high degree of variation in the mass of both P1 and F1 transgenic fish, with several fish of each generation exhibiting enhanced growth compared with other transgenic and control siblings. The 'six pack' phenotype observed in P1 transgenic RBT overexpressing acvr2b ∆ and the presence of F1 individuals with altered muscle morphology provides compelling evidence for the importance of TGF-β signaling molecules in regulating muscle growth in teleost fish.

show abstract

“…An increase in fiber number was observed in transgenic zebrafish over expressing Mstn1Pro (Xu et al, 2003). An improved growth of rainbow trout was observed by treatment with flatfish (Paralichthys olivaceus) Mstn1Pro expressed an E. coli system (Lee et al, 2010), indicating the potential of fish Mstn1Pro as an agent to improve the growth of commercially important aquaculture species. It is, therefore, desirable to develop a method for economic production of large quantity of fish Mstn1Pro.…”

Section: Introductionmentioning

confidence: 82%

“…An earlier study reported an insoluble expression of seabream Mstn1Pro in an E. coli system, thus a series of time-consuming refolding procedures were performed to obtain bioactive soluble Mstn1Pro (Funkenstein and Rebhan, 2007). Recently, however, bioactive fish Mstn1Pro proteins were expressed in soluble forms in an E. coli system using Maltose Binding Protein (MBP) as a fusion partner (Lee et al, 2010;2011), suggesting that MBP may serve as an useful fusion partner to induce soluble expression of MstnPro proteins of various species. However, the yield of purified protein was too low (about 700 µg L −1 culture) to realize the benefit of large production capacity of the E. coli expression system.…”

Section: Introductionmentioning

confidence: 99%

HIGH-YIELD PRODUCTION OF BIOACTIVE FLATFISH (<i>PARALICHTHYS OLIVACEUS</i>) MYOSTATIN-1 PRODOMAIN IN <i>ESCHERICHIA COLI</i>

Lee

Kim

Jin

et al. 2012

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Myostatin Propeptide (MstnPro), the N-terminal part of Mstn precursor molecule, binds to Mstn and suppress its activity. Mstn is a potent negative regulator of skeletal muscle growth in many animal species, thus MstnPro has a great potential to be used as an agent to enhance skeletal muscle growth in animal species. Bioactive fish Myostatin-1 Propeptide (Mstn1Pro) proteins were recently expressed in soluble forms in E. coli using Maltose Binding Protein (MBP) as a fusion partner. However, the yield of purified protein was too low to realize the benefit of large production capacity of the E. coli expression system. The objective of this study was to examine whether the yield of bioactive, recombinant flatfish (Paralichthys olivaceus) Mstn1Pro (poMstn1Pro) production can by improved through a proper selection of E. coli strain and other expression parameters. The poMstn1Pro gene construct, previously cloned into pMAL-c2 vector downstream of the Maltose-Binding Protein (MBP) gene, were transformed and expressed in E. coli K12BT1 strain. The MBP-poMstn1Pro protein was purified by the amylose-resin affinity chromatography. About 51 mg of soluble MBP-poMstn1Pro were purified by affinity chromatography from a liter culture. Pull-down assay and bioactivity test demonstrated the binding of MBP-poMstn1Pro to Mstn and its Mstn-suppressing capacity, indicating that the N-terminal fusion of MBP in the flatfish Mstn1Pro does not affect either the binding of flatfish Mstn1Pro to Mstn or its Mstn-suppressing activity. Results of this study demonstrate that a large production of MBP-poMstn1Pro is possible using an E. coli system to investigate the potential of MBP-poMstn1Pro to enhance muscle growth in aquaculture species or the binding characteristics of Mstn1Pro with its partners.

show abstract

Improving rainbow trout (Oncorhynchus mykiss) growth by treatment with a fish (Paralichthys olivaceus) myostatin prodomain expressed in soluble forms in E. coli

Cited by 25 publications

References 41 publications

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor

HIGH-YIELD PRODUCTION OF BIOACTIVE FLATFISH (<i>PARALICHTHYS OLIVACEUS</i>) MYOSTATIN-1 PRODOMAIN IN <i>ESCHERICHIA COLI</i>

Contact Info

Product

Resources

About