Improving reproducibility in spine research

Dahia, Chitra Lekha; Maitre, Christine L. Le

doi:10.1002/jsp2.1127

Cited by 1 publication

(2 citation statements)

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“…Lab‐to‐lab variability jeopardizes the much‐needed progress in the field. 32 In fact, multiple methodologies for IVD cell isolation and growing conditions are utilized within publications across all species. With, no consensus on isolation and culture methods to yield the highest IVD cell numbers and maintain viability and phenotype as close to in vivo as possible.…”

Section: Introductionmentioning

confidence: 99%

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Harmonization and standardization of nucleus pulposus cell extraction and culture methods

et al. 2023

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Background: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources. Methods:The most commonly applied methods for NP cell extraction, expansion, and re-differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re-differentiation media and techniques were also investigated.

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Section: Introductionmentioning

confidence: 99%

“…Lab‐to‐lab variability jeopardizes the much‐needed progress in the field 32 . In fact, multiple methodologies for IVD cell isolation and growing conditions are utilized within publications across all species.…”

Section: Introductionmentioning

confidence: 99%