Improving the colonization and functions of Wharton’s Jelly-derived mesenchymal stem cells by a synergetic combination of porous polyurethane scaffold with an albumin-derived hydrogel

Abstract

The development of neo-tissues assisted by artificial scaffolds is continually progressing, but the reproduction of the extracellular environment surrounding cells is quite complex. While synthetic scaffolds can support cell growth, they lack biochemical cues that can prompt cell proliferation or differentiation. In this study, Wharton’s Jelly-derived mesenchymal stem cells are seeded on a polyurethane (PU) scaffold combined with a hydrogel based on bovine serum albumin (BSA). BSA hydrogel is obtained through thermal treatment. While such treatment leads to partial unfolding of the protein, we show that the extent of denaturation is small enough to maintain its bioactivity, such as protein binding. Therefore, BSA provides a suitable playground for cells inside the scaffold, allowing higher spreading, proliferation and matrix secretions. Furthermore, the poor mechanical properties of the hydrogel are compensated for by the porous PU scaffold, whose architecture is well controlled. We show that even though PU by itself can allow cell adhesion and protein secretion, cell proliferation is 3.5 times higher in the PU + BSA scaffolds as compared to pure PU after 21 d, along with the non-collagenous protein secretions (389 versus 134 μmmg −1). Conversely, the secretion of sulphated glycosaminoglycans is 12.3-fold higher in the scaffold made solely of PU. Thereby, we propose a simple approach to generating a hybrid material composed of a combination of PU and BSA hydrogel as a promising scaffold for tissue regeneration.

Investigation of the Formation and Aging of Albumin-Based Condensates

“…20 Moreover, albumin-derived hydrogels can stimulate cell adhesion and colonization into biomaterials. 21 The ability of albumin to undergo LLPS was evidenced through coacervation with polysaccharides as reported by Huang et al, 22 for pH comprised between 2 and 4. In another study, BSA formed coacervates with chondroitin sulfate at pH = 4.2.…”

“…Likewise, Zhao et al used bovine serum albumin (BSA) to transport paclitaxel, while the supply of apolipoprotein E into the brain was shown to be substantially enhanced through binding to albumin . Moreover, albumin-derived hydrogels can stimulate cell adhesion and colonization into biomaterials …”