Physical activity, especially routine walking, is an imperative factor for the prevention of strokes, mental illness, and cardiovascular diseases (CVDs). The NIH (National Institute of Health) has also acknowledged walking as the most important factor of the stroke rehabilitation program. Many research studies are presented by physicians and researchers in the literature that highlight the positive impacts of walking on human health (physical and mental). This paper has the objective of studying the impact of regular walking, especially on mental illness, CVDs, and strokes. The C-reactive protein (CRP), P-selectin protein, and homocysteine biomarkers are considered to decide the improvement in the health of an individual with respect to CVDs and strokes. The other parameters considered for the recommendations of physicians and healthcare experts for mental health are PSS (perceived stress score) and ESS (Epworth sleepiness score) that control mental illness. The values are measured for the participating subjects before participating in the walking activity and after the end of the walking schedule to see the impact on individuals. The overall mental and physical health of an individual contributes to the chances of occurrence of CVDs, mental illness, and strokes in individuals aged between 40 years and 55 years, as per the study presented in this paper. The results show that the PSS and ESS scores are improved after the walking activity. Eventually, it improved recovery from many kinds of mental illness and also reduced the chances of strokes. Similarly, the levels of the biomarkers that determine the chances of an individual having CVD or stroke also improved. Walking can impact our overall health in many ways, however, in this paper, the focus is given to ailments, such as strokes, CVDs, and mental illness. The results show that stress and improper sleepiness can impact mental health negatively. The research outcome is measured by adding walking in a routine life so that every individual can get rid of many physical and mental ailments. The results presented in the paper reveal that the 90-day walking program has created a good impact on the health of individuals by improving their physical and mental health.