Improving the enzymolysis efficiency of lupin protein by ultrasound pretreatment: Effect on antihypertensive, antidiabetic and antioxidant activities of the hydrolysates

Fadimu, Gbemisola J.; Gill, Harsharn; Farahnaky, Asgar; Truong, Tuyen

doi:10.1016/j.foodchem.2022.132457

Cited by 39 publications

(36 citation statements)

References 38 publications

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“…The highest α-amylase inhibitory activity was observed in unfractionated hydrolysates generated using alcalase (ACT) (IC50 value = 1.66 mg/mL), followed by unfractionated hydrolysates from flavourzyme (FCT) (IC50 value = 1.98 mg/mL). This result is in accordance with previous studies which reported that hydrolysates generated using alcalase had significantly higher α-amylase inhibitory (lower IC50 value) ability than those prepared using flavourzyme, based on IC50 values [4,10].…”

Section: Inhibition Of the α-Amylase Inhibitory Activitysupporting

confidence: 93%

In-Silico Analysis and Antidiabetic Effect of α-Amylase and α-Glucosidase Inhibitory Peptides from Lupin Protein Hydrolysate: Enzyme-Peptide Interaction Study Using Molecular Docking Approach

Fadimu

Farahnaky

Gill

et al. 2022

Foods

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The use of natural ingredients for managing diabetes is becoming more popular in recent times due to the several adverse effects associated with synthetic antidiabetic medications. In this study, we investigated the in vitro antidiabetic potential (through inhibition of α-glucosidase (AG) and α-amylase (AA)) of hydrolysates from lupin proteins pretreated with ultrasound and hydrolyzed using alcalase (ACT) and flavourzyme (FCT). We further fractionated ACT and FCT into three molecular weight fractions. Unfractionated ACT and FCT showed significantly (p < 0.05) higher AG (IC50 value = 1.65 mg/mL and 1.91 mg/mL) and AA (IC50 value = 1.66 mg/mL and 1.98 mg/mL) inhibitory activities than their ultrafiltrated fractions, where lower IC50 values indicate higher inhibitory activities. Then, ACT and FCT were subjected to peptide sequencing using LC-MS-QTOF to identify the potential AG and AA inhibitors. Molecular docking was performed on peptides with the highest number of hotspots and PeptideRanker score to study their interactions with AG and AA enzymes. Among the peptides identified, SPRRF, FE, and RR were predicted to be the most active peptides against AG, while AA inhibitors were predicted to be RPR, PPGIP, and LRP. Overall, hydrolysates prepared from lupin proteins using alcalase and flavourzyme may be useful in formulating functional food for managing diabetics.

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Section: Inhibition Of the α-Amylase Inhibitory Activitysupporting

confidence: 93%

In-Silico Analysis and Antidiabetic Effect of α-Amylase and α-Glucosidase Inhibitory Peptides from Lupin Protein Hydrolysate: Enzyme-Peptide Interaction Study Using Molecular Docking Approach

Fadimu

Farahnaky

Gill

et al. 2022

Foods

Self Cite

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show abstract

“…In general, ultrasound-assisted enzymolysis can improve the antioxidant activity of enzymatic hydrolysates. Many related studies have been reported [41] , [42] , [43] , [44] . For example, Wen et al [42] showed that the antioxidant activity of arrowhead hydrolysates was improved by slit divergent ultrasound.…”

Section: Resultsmentioning

confidence: 99%

Effects of dual-frequency slit ultrasound on the enzymolysis of high-concentration hydrolyzed feather meal: Biological activities and structural characteristics of hydrolysates

Hong

Zhu

Zhao

et al. 2022

Ultrasonics Sonochemistry

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“…The DPPH radical scavenging activity of SPIN was 35.981 %, while increased to 38.743 %–39.821 % after ultrasound pretreatment. It has been indicated that the antioxidant capacity was mainly determined by low molecular weight peptides, and ultrasound cavitation led to the release of low molecular weight peptides, which were more likely to interact with free radicals [51] . Enhanced antioxidant capacity might also be due to protein unfolding promoted by ultrasound, exposing hidden amino acid residues and side chains with antioxidant capacity, which were normally hidden in the three-dimensional structure of protein molecules [52] .…”

Section: Resultsmentioning

confidence: 99%

Effects of ultrasound pretreatment on functional property, antioxidant activity, and digestibility of soy protein isolate nanofibrils

2022

Ultrasonics Sonochemistry

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Improving the enzymolysis efficiency of lupin protein by ultrasound pretreatment: Effect on antihypertensive, antidiabetic and antioxidant activities of the hydrolysates

Cited by 39 publications

References 38 publications

In-Silico Analysis and Antidiabetic Effect of α-Amylase and α-Glucosidase Inhibitory Peptides from Lupin Protein Hydrolysate: Enzyme-Peptide Interaction Study Using Molecular Docking Approach

In-Silico Analysis and Antidiabetic Effect of α-Amylase and α-Glucosidase Inhibitory Peptides from Lupin Protein Hydrolysate: Enzyme-Peptide Interaction Study Using Molecular Docking Approach

Effects of dual-frequency slit ultrasound on the enzymolysis of high-concentration hydrolyzed feather meal: Biological activities and structural characteristics of hydrolysates

Effects of ultrasound pretreatment on functional property, antioxidant activity, and digestibility of soy protein isolate nanofibrils

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