Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents

Alriksson, Björn; Cavka, Adnan; Jönsson, Leif J.

doi:10.1016/j.biortech.2010.08.037

Cited by 137 publications

(91 citation statements)

References 31 publications

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“…Furthermore, a method for group analysis of phenols by high-performance liquid chromatography (HPLC) has also been used [38]. Although the FolinCiocalteu method is the most convenient approach to analyze the total phenolic contents in lignocellulose hydrolysates, it should be avoided in experiments with redox reagents (such as reduced sulfur compunds including dithionite, dithiothreitol, and sulfite), in which the HPLC method serves as a better option [39]. It should also be noticed that phenol analysis using the Folin-Ciocalteu reagent is related to the Lowry method for determination of the total protein content [40] and that it is therefore sensitive to potential media components such as hydrolytic enzymes, cell extracts, and hydrolyzed protein.…”

Section: Aromatic Compoundsmentioning

confidence: 99%

- Synthetic Biology: A Promising Technology for Biofuel Production

2015

New Biotechnologies for Increased Energy Security

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Section: Aromatic Compoundsmentioning

confidence: 99%

- Synthetic Biology: A Promising Technology for Biofuel Production

2015

New Biotechnologies for Increased Energy Security

View full text Add to dashboard Cite

“…Solvents (e.g., ethyl acetate) and active charcoal and/or ion-exchange resins reduce the concentration of inhibitors by extraction or adsorption, respectively [38][39][40]. Chemical transformation of inhibitors is also possible by addition of reducing agents (dithionite and sulfite) [41] and chemical catalysts, being overliming (treatment with Ca(OH) 2 ) the most efficient chemical detoxification method for removing phenols and furan derivatives [37].…”

Section: Detoxification Of Pretreated Materialsmentioning

confidence: 99%

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

et al. 2017

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Abstract:The continuous increase in the world energy and chemicals demand requires the development of sustainable alternatives to non-renewable sources of energy. Biomass facilities and biorefineries represent interesting options to gradually replace the present industry based on fossil fuels. Lignocellulose is the most promising feedstock to be used in biorefineries. From a sugar platform perspective, a wide range of fuels and chemicals can be obtained via microbial fermentation processes, being ethanol the most significant lignocellulose-derived fuel. Before fermentation, lignocellulose must be pretreated to overcome its inherent recalcitrant structure and obtain the fermentable sugars. Usually, harsh conditions are required for pretreatment of lignocellulose, producing biomass degradation and releasing different compounds that are inhibitors of the hydrolytic enzymes and fermenting microorganisms. Moreover, the lignin polymer that remains in pretreated materials also affects biomass conversion by limiting the enzymatic hydrolysis. The use of laccases has been considered as a very powerful tool for delignification and detoxification of pretreated lignocellulosic materials, boosting subsequent saccharification and fermentation processes. This review compiles the latest studies about the application of laccases as useful and environmentally friendly delignification and detoxification technology, highlighting the main challenges and possible ways to make possible the integration of these enzymes in future lignocellulose-based industries.

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“…As a result, it was possible to devise an approach for determining the best detoxification technique or combination of techniques suitable in every single case from a technical point of view. In recent years, many publications have studied a single detoxification technique coupled with a specific fermentation scenario [2,9,16,19,23,35]. Other papers have studied the combination of detoxification techniques experimentally [10,17,18,20,21,36,37] or from a theoretical viewpoint [26,31,38,39].…”

Section: Introductionmentioning

confidence: 99%

Detoxification of a Lignocellulosic Waste from a Pulp Mill to Enhance Its Fermentation Prospects

2017

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Detoxification is required for sugar bioconversion and hydrolyzate valorization within the biorefining concept for biofuel or bio-product production. In this work, the spent sulfite liquor, which is the main residue provided from a pulp mill, has been detoxified. Evaporation, overliming, ionic exchange resins, and adsorption with activated carbon or black carbon were considered to separate the sugars from the inhibitors in the lignocellulosic residue. Effectiveness in terms of total and individual inhibitor removals, sugar losses and sugar-to-inhibitor removal ratio was determined. The best results were found using the cation exchange Dowex 50WX2 resin in series with the anion exchange Amberlite IRA-96 resin, which resulted in sugar losses of 24.2% with inhibitor removal of 71.3% of lignosulfonates, 84.8% of phenolics, 82.2% acetic acid, and 100% of furfurals. Apart from exchange resins, the results of evaporation, overliming, adsorption with activated carbon and adsorption with black carbon led to total inhibitor removals of 8.6%, 44.9%, 33.6% and 47.6%, respectively. Finally, some fermentation scenarios were proposed in order to evaluate the most suitable technique or combination of techniques that should be implemented in every case.

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Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents

Cited by 137 publications

References 31 publications

- Synthetic Biology: A Promising Technology for Biofuel Production

- Synthetic Biology: A Promising Technology for Biofuel Production

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

Detoxification of a Lignocellulosic Waste from a Pulp Mill to Enhance Its Fermentation Prospects

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