Improving the quality of ovine embryo produced in vitro by culturing zygote in isolated mouse oviduct

Farahavar, Abbas; Shirazi, Abolfazl; Kohram, Hamid; Sarvari, Ali; Borjian-Boroujeni, Sara; Naderi, Mohammad; Shahneh, A. Z.; Zhandi, Mahdi

doi:10.1016/j.smallrumres.2017.11.004

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…It is reported that embryos developed by ICSI and SCNT methods and even frozen embryos showed a high rate of H3K9me3 and me3H3K4 methylation. These genomic changes profoundly affected the gene expression levels in these embryos (21)(22)(23). The Me3 H3K4 accumulates at the transcription start site of genes involved in embryonic development such as pleuropotency genes, and its reduction leads to a decrease in the expression of genes such as Nanog, Oct4 and Sox2.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic Events and Germ Line Transmission in Rag1 knock-out Chimeric Mice Produced by CRISPR/Cas9 Technology

Salimi

Mehravar

Shirazi

et al. 2021

Preprint

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Considering the effects of epigenetic changes on the embryonic development through altering the gene expression of pluripotency, trophectoderm, and imprinting genes, we determined the pattern of epigenetic alterations in transgenic embryos produced by injecting transgenic mESCs into the morula. To aim this, RAG1 knock-out mESCs were produced using CRISPR-Cas9 editing system and then microinjected into morulas to develop chimeric embryos. Afterward, the expression of pluripotency, trophectoderm genes, and imprinting genes in these embryos were evaluated using real-time PCR. Immunohistochemical analysis was carried out to determine the methylation rates of H3K9me3 and H3K4me3. In the following, since epigenetic alterations can be at least one of the possible reasons of male infertility and the loss of germ-line transmission capacity, the germ-line transmission ability in chimeric mice was also evaluated by breeding them and subsequent backcrosses to wild type strains. Our findings showed that the methylation rates of H3K4me3 and H3K9me3 were significantly lower and higher respectively in the RAG1 knock-out embryos compared others groups. Moreover, the chimeric embryos exhibited the decreased expression of NANOG, OCT4, CDX2, TEAD4, and H19 genes. Following the breeding of chimeric males with normal female mouse, 40% of chimeras had no germ-line transmission (GLT), and 60% were infertile.We showed that the manipulation of mESC by CRISPR-Cas9 approach remarkably changed the methylation status of H3K9me3 and H3K4me3, resulting in impaired development of embryos through dysregulation of genes involved embryonic development and then, may be one of the reasons of infertility and lack of GLT.

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Section: Discussionmentioning

confidence: 99%

Epigenetic Events and Germ Line Transmission in Rag1 knock-out Chimeric Mice Produced by CRISPR/Cas9 Technology

Salimi

Mehravar

Shirazi

et al. 2021

Preprint

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“…Although ROS can regulate cell function and activate key signaling pathways, increased ROS can cause the formation of highly radial hydroxyl which can increase lipid peroxidation, increase DNA oxidation and damage DNA nuclei 10 , increase protein oxidation 11 .…”

Section: Discussionmentioning

confidence: 99%

Moringa Leaf (Moringa oleifera Lam) Nanoparticle Supplementation on Zygote Cleavage in Goat Embryo Culture In Vitro

2020

IJFMT

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One of the causes of failure of zygote development is oxidative stress in the culture medium. Where oxidative stress can damage the structure and function of zygotes due to free radicals Reactive Oxygen Species (ROS). Moringa oleifera Lam contains antioxidant polyphenol compounds, flavonoid flavanols namely quercetin which is the most antioxidant content in Moringa leaves which is expected to fight excess ROS. The purpose of this study was to prove that the supplementation of Moringa oleifera Lam leaf nanoparticles to increase zygote cleavage in goat embryo culture in vitro. The research subjects used goat oocytes which were divided into 4 groups consisting of 1 control group and 3 treatment groups. Each group consisted of 31 samples. Control group K1 without Moringa leaf nanoparticle supplementation, treatment group I P1 Moringa leaf nanoparticle supplementation with a dose of 0.5 µM, treatment group II P2 supplementation of Moringa leaf nanoparticles with a dose of 1.0 µM, Treatment group III P3 supplementation with Moringa leaf 2.0 µM. Embryo culture was carried out in a 5% CO2 incubator, temperature 38.5ºC for 48 hours, then observed under an inverted microscope. The results show that there are significant differences with the value of p = 0.041 which means p <0.05. Supplementation of moringa leaf nanoparticles (Moringa oleifera Lam) can significantly increase zygote cleavage at embryo culture stage in vitro.

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Improving the quality of ovine embryo produced in vitro by culturing zygote in isolated mouse oviduct

Cited by 2 publications

References 27 publications

Epigenetic Events and Germ Line Transmission in Rag1 knock-out Chimeric Mice Produced by CRISPR/Cas9 Technology

Epigenetic Events and Germ Line Transmission in Rag1 knock-out Chimeric Mice Produced by CRISPR/Cas9 Technology

Moringa Leaf (Moringa oleifera Lam) Nanoparticle Supplementation on Zygote Cleavage in Goat Embryo Culture In Vitro

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