Improving the remaining activity of lignocellulolytic enzymes by membrane entrapment

Recent Developments in the Delignification and Exploitation of Grass Lignocellulosic Biomass

“…In contrast, LC immobilization has widely been investigated. − Bilal et al reported the use of a ligninolytic enzyme cocktail from Ganoderma lucidum IBL-05 immobilized on alginate–chitosan beds to delignify sorghum stover, obtaining a delignification of 57.3% after 15 h. The reaction was carried out in 50 mM sodium malonate buffer (pH 4.5) and is relevant as delignification proceeds without the use of mediators . Chang et al studied the entrapment of laccase from the lacquer tree Rhus vernicifera in a cellulose ester membrane, finding that the immobilized LC had much higher levels of retained activity (20.34% after 2 days) compared with the free LC . Amin et al immobilized LC from Trametes versicolor by covalent attachment on modified Fe 3 O 4 @SiO 2 @Kit-6 magnetite nanoparticles in the presence of IL for enhanced delignification of olive pomace biowaste .…”

Enzyme catalysis coupled with artificial membranes towards process intensification in biorefinery- a review

“…However, since enzyme immobilization can contribute to the development of sustain able processes, it has substantial potential to be used in industrial lignocellulose-to-ethanol conversion. (Chang et al, 2011;Rodrigues et al, 2017) A very recent strategy of biocatalytic systems is to immobilize en zymes on superparamagnetic nanoparticles (NP SP )_ These particles af terwards are reversibly immobilized on a microporous membrane using an external magnetic field in a system named superparamagnetic bio catalytic membrane reactor (BMR sp ) (Gebreyohannes et al, 2015(Gebreyohannes et al, , 2017. The immobilization of the enzyme on me NP sp can improved stability, activity along with easy recovery using an external magnetic force.…”

Cellulose-deconstruction potential of nano-biocatalytic systems: A strategic drive from designing to sustainable applications of immobilized cellulases

“…Similarly, Chang et al [69] developed silica nanoparticles of ultra large pore (20-40 nm) and small pore size of 2-5 nm. Dimethyl phthalate as pore expander was utilized for the formation of larger pore sized materials by co-condensation with 3aminopropyltrimethoxysilane.…”

“…The presence of both Si-OH and Si-NH2 groups provided the larger pore size to the silica supports than cellulase molecule size. Therefore, electrostatic interaction between cellulase and Si-NH2 enabled easy physical adsorption [69].…”