Improving the resolution of chromosome imaging by high numerical aperture Fourier ptychography microscopy

Using symmetric illumination and color camera to achieve high throughput Fourier ptychographic microscopy

“…Fortunately, the recently introduced Fourier ptychographic microscopy (FPM) [5][6][7] method provides an effective way to improve both SBP and DOF of a conventional microscope. By using a low-cost LED panel and a phase retrieval algorithm, FPM can computationally integrate a series of low-resolution images captured under diverse illumination angles into a single high-resolution image [5,[8][9][10][11][12], while successfully inheriting the larger field of view (FOV) and DOF [13] from the adopted low-NA objective lens. Correspondingly, the SBP can be enlarged by more than 10 times while lowering the possibility of re-scanning the sample due to inaccurate focusing as a result of the larger DOF.…”