Improving the sensitivity of bacterial bioreporters for heavy metals

Hynninen, Anu; Tõnismann, Karmen; Virta, Marko

doi:10.4161/bbug.1.2.10902

Cited by 63 publications

(35 citation statements)

References 29 publications

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“…Bioreporter induction was reported as an induction coefficient (IC), calculated as IC=LM/LW, where LM is the luminescence in the metal solution and LW is the background luminescence measured in water. The limit of detection was determined according to the scheme presented by (10).…”

Section: Bioavailability Of Mercurymentioning

confidence: 99%

Fish Farming Affects the Abundance and Diversity of the Mercury Resistance Gene merA in Marine Sediments

et al. 2011

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Microbes in sediments below fish farming cages are exposed to a variety of compounds used in aquaculture, such as antifoulants, antimicrobials and mercury. Here, we assessed the abundance of the mercury resistance gene merA in the sediment of four fish farms and four pristine sites using quantitative PCR, and at three fish farms and two pristine sites by constructing clone libraries and sequencing merA amplicons. The abundance of merA was consistently greater at fish farm sites than at pristine sites, and the merA phylotypes were different between fish farm and pristine sites. Mercury concentrations were too low to cause selection pressure and did not differ significantly among samples. The primers designed specifically for this study were capable of detecting two new potential merA clades.

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Section: Bioavailability Of Mercurymentioning

confidence: 99%

Fish Farming Affects the Abundance and Diversity of the Mercury Resistance Gene merA in Marine Sediments

et al. 2011

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“…This was achieved by using promoter of the CadA1 gene from Pseudomonas putida as a sensor element for constructing a Zn-specific bioreporter. Thus, a Zn 2+ concentration about 50 times lower than that detected by available Zn-based biosensors was achieved [96]. Therefore, whole-cell-based biosensors are emerging as an inexpensive and easy option for detecting heavy metal detection and monitoring.…”

Section: Desired Community and Its Performancementioning

confidence: 99%

“…Extraction of specific methyl ester profiles of microorganisms [22], [23] Quantitative PCR (qPCR) and RT-Q-PCR DNA/RNA Based on PCR, simultaneously amplifies and quantifies DNA/ RNA [27] Microbial degradative analysis Quantitative PCR (qPCR) and RT-Q-PCR DNA/RNA Based on PCR, simultaneously amplifies and quantifies DNA/ RNA [73], [74] DNA microarray DNA Arrays of DNA that hybridizes with specific target DNA strand [94] Monitoring of community structure Biosensors Nutrients like nitrogen, phosphorus, pollutants, toxic compounds Use of specific bioactive compound which emits signals [50], [93], [94], [95], [96] structures can be studied and compared by using DGGE profiles of PCR amplified products (16S and 18S rRNA genes) [17]. In addition, highly sensitive technique like terminal restriction fragment length polymorphism (T-RFLP) is also used for environmental samples [18].…”

Section: Methyl Esters From Lipidsmentioning

confidence: 99%

Integrated Perspective for Effective Bioremediation

Paliwal

Puranik

Purohit

2011

Appl Biochem Biotechnol

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Identification of factors which can influence the natural attenuation process with available microbial genetic capacities can support the bioremediation which has been viewed as the safest procedure to combat with anthropogenic compounds in ecosystems. With the advent of molecular techniques, assimilatory capacity of an ecosystem can be defined with changing community dynamics, and if required, the essential genetic potential can be met through bioaugmentation. At the same time, intensification of microbial processes with nutrient balancing, expressing and enhancing the degradative capacities, could reduce the time frame of restoration of the ecosystem. The new concept of ecosystems biology has added greatly to conceptualize the networking of the evolving microbiota of the niche that helps in effective application of bioremediation tools to manage pollutants as additional carbon source.

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“…By fusing the appropriate stress-responsive gene promoters upstream to reporting gene systems, the present authors and others have constructed bacterial strains designed to report on cellular stresses other than DNA damage, including protein misfolding, fatty acid synthesis inhibition, an increased production of reactive oxygen species, and an excessive presence of metals (Ahn et al, 2010;Belkin et al, 1997;Ben-Israel et al, 1998;Hynninen et al, 2010;Ivask et al, 2009;Vandyk et al, 1994). Moreover, advances in robotics have prompted the assembly of comprehensive bacterial bioreporter collections that cover a substantial fraction of the gene promoters in a given test bacterium.…”

Section: Introductionmentioning

confidence: 95%

High-throughput prescreening of pharmaceuticals using a genome-wide bacterial bioreporter array

Elad

Seo

Belkin

et al. 2015

Biosensors and Bioelectronics

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We assessed the applicability of multi-strain bacterial bioreporter bioassays to drug screening. To this end, we investigated the reactions of a panel of 15 luminescent recombinant Escherichia coli bacterial bioreporters to a library of 420 pharmaceuticals. The panel included bacterial bioreporters associated with oxidative stress, DNA damage, heat shock, and efflux of excess metals. Eighty nine drugs elicited a response from at least one of the panel members and formed distinctive clusters, some of which contained closely related drugs. In addition, we tested a group of selected nine drugs against a collection of about 2000 different fluorescent transcriptional reporters that covers the great majority of gene promoters in E. coli. The sets of induced genes were in accord with the in vitro toxicity of the tested drugs, as reflected by the response patterns of the 15-member panel, and provided more insights into their toxicity mechanisms. Facilitated by microplates and robotic systems, all assays were conducted in high-throughput. Our results thus suggest that multi-strain assemblages of bacterial bioreporters have the potential for playing a significant role in drug development alongside current in vitro toxicity tests.

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Improving the sensitivity of bacterial bioreporters for heavy metals

Cited by 63 publications

References 29 publications

Fish Farming Affects the Abundance and Diversity of the Mercury Resistance Gene merA in Marine Sediments

Fish Farming Affects the Abundance and Diversity of the Mercury Resistance Gene merA in Marine Sediments

Integrated Perspective for Effective Bioremediation

High-throughput prescreening of pharmaceuticals using a genome-wide bacterial bioreporter array

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