Improving the stability of glutamate fermentation by Corynebacterium glutamicum via supplementing sorbitol or glycerol

Cao, Yan; He, Zhen-ni; Shi, Zhongping; Mpofu, Enock

doi:10.1186/s40643-014-0032-6

Cited by 2 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glucose, glycerol, 1,3-PDO and other organic acids were quantified by using High performance liquid chromatography (HPLC) equipped with an Aminex HPX-87H Column (300 × 7.8 mm) using 0.005 M H 2 SO 4 as the mobile phase with a flow rate of 0.6 mL/min, and detection via refractive index or UV absorption at 210 nm 52 . Glutamate concentration was determined by a glutamate biosensor (SBA-40C, Shandong Science Academy, Jinan, China) 53 . The concentration of 3-HPA was measured according to the method described by Krauter et al .…”

Section: Methodsmentioning

confidence: 99%

Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum

Huang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Production of 1,3-propanediol (1,3-PDO) from glycerol is a promising route toward glycerol biorefinery. However, the yield of 1,3-PDO is limited due to the requirement of NADH regeneration via glycerol oxidation process, which generates large amounts of undesired byproducts. Glutamate fermentation by Corynebacterium glutamicum is an important oxidation process generating excess NADH. In this study, we proposed a novel strategy to couple the process of 1,3-PDO synthesis with glutamate production for cofactor regeneration. With the optimization of 1,3-PDO synthesis route, C. glutamicum can efficiently convert glycerol into 1,3-PDO with the yield of ~ 1.0 mol/mol glycerol. Co-production of 1,3-PDO and glutamate was also achieved which increased the yield of glutamate by 18% as compared to the control. Since 1,3-PDO and glutamate can be easily separated in downstream process, this study provides a potential green route for coupled production of 1,3-PDO and glutamate to enhance the economic viability of biorefinery process.

show abstract

Section: Methodsmentioning

confidence: 99%

Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum

Huang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

Review of the Proteomics and Metabolic Properties of Corynebacterium glutamicum

Park,

Lim

2024

Microorganisms

View full text Add to dashboard Cite

Corynebacterium glutamicum (C. glutamicum) has become industrially important in producing glutamic acid and lysine since its discovery and has been the subject of proteomics and central carbon metabolism studies. The proteome changes depending on environmental conditions, nutrient availability, and stressors. Post-translational modification (PTMs), such as phosphorylation, methylation, and glycosylation, alter the function and activity of proteins, allowing them to respond quickly to environmental changes. Proteomics techniques, such as mass spectrometry and two-dimensional gel electrophoresis, have enabled the study of proteomes, identification of proteins, and quantification of the expression levels. Understanding proteomes and central carbon metabolism in microorganisms provides insight into their physiology, ecology, and biotechnological applications, such as biofuels, pharmaceuticals, and industrial enzyme production. Several attempts have been made to create efficient production strains to increase productivity in several research fields, such as genomics and proteomics. In addition to amino acids, C. glutamicum is used to produce vitamins, nucleotides, organic acids, and alcohols, expanding its industrial applications. Considerable information has been accumulated, but recent research has focused on proteomes and central carbon metabolism. The development of genetic engineering technologies, such as CRISPR-Cas9, has improved production efficiency by allowing precise manipulation of the metabolic pathways of C. glutamicum. In addition, methods for designing new metabolic pathways and developing customized strains using synthetic biology technology are gradually expanding. This review is expected to enhance the understanding of C. glutamicum and its industrial potential and help researchers identify research topics and design studies.

show abstract

Improving the stability of glutamate fermentation by Corynebacterium glutamicum via supplementing sorbitol or glycerol

Cited by 2 publications

References 25 publications

Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum

Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum

Review of the Proteomics and Metabolic Properties of Corynebacterium glutamicum

Contact Info

Product

Resources

About