2020
DOI: 10.1016/j.ab.2020.113681
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Improving the stability of the malachite green method for the determination of phosphate using Pluronic F68

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Cited by 16 publications
(23 citation statements)
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“…In the original work, Kennedy and coworkers (1) measured the production of Pi from PA by a colorimetric assay with the molybdate reagent first described in 1925 by Fiske and Subbarow (98). The sensitivity and stability of the molybdate reagent for measuring PAP activity has been improved by its combination with malachite green (12,63,(99)(100)(101)(102). The Pi produced in the PAP assay is measured after the removal of the enzyme by precipitation with trichloroacetic acid, which is also used to terminate the reaction (1).…”
Section: Nonradioactive Assaysmentioning
confidence: 99%
“…In the original work, Kennedy and coworkers (1) measured the production of Pi from PA by a colorimetric assay with the molybdate reagent first described in 1925 by Fiske and Subbarow (98). The sensitivity and stability of the molybdate reagent for measuring PAP activity has been improved by its combination with malachite green (12,63,(99)(100)(101)(102). The Pi produced in the PAP assay is measured after the removal of the enzyme by precipitation with trichloroacetic acid, which is also used to terminate the reaction (1).…”
Section: Nonradioactive Assaysmentioning
confidence: 99%
“…Malachite green (MG) methods belong to a family of analytical methods based on the formation of 12-molybdophosphoric acid (12-MPA) by the reaction between Pi and molybdate in strong acid solution. 2 These methods are the best choice for quantifying ATPase activities for mechanistic studies.…”
Section: Introductionmentioning
confidence: 99%
“…In these conditions a green nanoparticle with distinctive spectroscopic properties, including a high molar absorption coefficient in the order of 94 000 M −1 cm −1 at 640 nm, is formed. 2…”
Section: Introductionmentioning
confidence: 99%
“…However, the Molybdenum blue-based spectrophotometry method introduced by Murphy and Riley [20] and improved by successive researchers [21][22][23] has been broadly accepted as a standard method for the determination of phosphate in water [1]. In this method, ammonium molybdate in a strong acidic condition reacts with orthophosphate (Equation ( 1)) to form a Keggin ion [PMo 12 O 40 ] 3− and then is reduced by ascorbic acid (Equation ( 2)) to generate the molybdenum blue complex [24]. Potassium antimonyl tartrate, as a source of antimony, has been frequently used for increasing the rate of reaction and eliminating the need for a heating process to form the stable molybdenum blue product [25][26][27].…”
Section: Introductionmentioning
confidence: 99%
“…Potassium antimonyl tartrate, as a source of antimony, has been frequently used for increasing the rate of reaction and eliminating the need for a heating process to form the stable molybdenum blue product [25][26][27]. A wide range of analytical methods have been developed for phosphate concentration in aquatic samples so far, including electrochemical analysis [10,11], spectrophotometry [12,13], colorimetry [14,15], fluorometry [16,17], and enzymatic biosensors [18,19]. However, the Molybdenum blue-based spectrophotometry method introduced by Murphy and Riley [20] and improved by successive researchers [21][22][23] has been broadly accepted as a standard method for the determination of phosphate in water [1].…”
Section: Introductionmentioning
confidence: 99%