Improving Tumor Targeting of Exosomal Membrane-Coated Polymeric Nanoparticles by Conjugation with Aptamers

Han, Zhaobin; Lv, Wenxing; Li, Yike; Chang, Jianqiao; Zhang, Wei; Liu, Chao; Sun, Jiashu

doi:10.1021/acsabm.0c00181

Cited by 54 publications

(44 citation statements)

References 47 publications

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“… 86 Active targeting can be achieved ( E ) by using specific targeting moiety to bind to the receptors on tumor cells or by modification to synergize external stimuli-guided tumor targeting. 93 , 95 , 101 , 102 , 105 . …”

Section: Strategies To Improve Tumor Targeting Of Exosomementioning

confidence: 99%

Section: Cancer Cell Selectivitymentioning

confidence: 99%

“…100 In one study, cholesterol modified aptamer AS1411 conferred exosome enhanced tumor targeting in an MDA-MB -231-xenografted mouse model due to specific binding of AS1411 aptamers to nucleolin on breast cancer cells. 101 Surface modification of exosomes also allows for 2-in-1 or all-in-1 strategies to achieve tumor specific-killing. The synergistic tumor-killing effect has been demonstrated in the study of engineered Cas9 vector-encapsulated exosomes with the surface expression of tumor necrosis factor (TNFα).…”

mentioning

confidence: 99%

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<p>Advances in Exosome-Based Drug Delivery and Tumor Targeting: From Tissue Distribution to Intracellular Fate</p>

Shao

Zaro

Shen

2020

IJN

188

100

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Exosomes or small extracellular vesicles are considered a new generation of bioinspired-nanoscale drug delivery system (DDS). Endogenous exosomes function as signalosomes since they convey signals via ligands or adhesion molecules located on the exosomal membrane, or packaged inside the exosome. Recently, exosome membrane modification, therapeutic payloads encapsulation, and modulation of in vivo disposition of exosomes have been extensively investigated, among which significant advances have been made to optimize exosome-mediated delivery to solid tumors. Exosomes, specifically tumor cell-derived exosomes, are presumed to have tumor-preferential delivery due to the homotypic features. However, quality attributes that dictate the tissue distribution, cell typeselective uptake, and intracellular payload release of the administered exosomes, as well as the spatiotemporal information regarding exosome fate in vivo, remain to be further investigated. This review summarizes recent advances in developing exosomes as drug delivery platforms with a focus on tumor targeting. The pharmacokinetic features of naive exosomes and factors influencing their intracellular fate are summarized. Recent strategies to improve tumor targeting of exosomes are also reviewed in the context of the biological features of tumor and tumor microenvironment (TME). Selected approaches to augment tumor tissue deposition of exosomes, as well as methods to enhance intracellular payload delivery, are summarized with emphasis on the underlying mechanisms (eg, passive or active targeting, endosomal escape, etc.). In conclusion, this review highlights recently reported tumortargeting strategies of exosome-based drug delivery, and it's in the hope that multiple approaches might be employed in a synergistic combination in the development of exosomebased cancer therapy.

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Section: Strategies To Improve Tumor Targeting Of Exosomementioning

confidence: 99%

Section: Cancer Cell Selectivitymentioning

confidence: 99%

mentioning

confidence: 99%

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<p>Advances in Exosome-Based Drug Delivery and Tumor Targeting: From Tissue Distribution to Intracellular Fate</p>

Shao

Zaro

Shen

2020

IJN

188

100

View full text Add to dashboard Cite

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“…HDNPs and photoresponsive drug release of DOX caused significant inhibition of the cancer cell proliferation. In a recent study of Han et al [ 219 ] the PNPs coated with exosomal membrane (EM-coated PLGA) functionalized with DNA aptamers AS1411 were reported ( Figure 12 ). The EM coating was not affected by the aptamer modification and the proposed PNPs indicated good biocompatibility and efficient tumor targeting.…”

Section: Polymer Nanoparticles Modified By Aptamers and Antibodiesmentioning

confidence: 99%

“…The PNPs were characterized by reduced uptake by macrophages, and by enhanced targeting to tumor cells with overexpressed nucleolin. Reproduced from Han et al [ 219 ] with permission of American Chemical Society.…”

Section: Figurementioning

confidence: 99%

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

2021

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Polymer nanoparticles and nano/micromotors are novel nanostructures that are of increased interest especially in the diagnosis and therapy of cancer. These structures are modified by antibodies or nucleic acid aptamers and can recognize the cancer markers at the membrane of the cancer cells or in the intracellular side. They can serve as a cargo for targeted transport of drugs or nucleic acids in chemo- immuno- or gene therapy. The various mechanisms, such as enzyme, ultrasound, magnetic, electrical, or light, served as a driving force for nano/micromotors, allowing their transport into the cells. This review is focused on the recent achievements in the development of polymer nanoparticles and nano/micromotors modified by antibodies and nucleic acid aptamers. The methods of preparation of polymer nanoparticles, their structure and properties are provided together with those for synthesis and the application of nano/micromotors. The various mechanisms of the driving of nano/micromotors such as chemical, light, ultrasound, electric and magnetic fields are explained. The targeting drug delivery is based on the modification of nanostructures by receptors such as nucleic acid aptamers and antibodies. Special focus is therefore on the method of selection aptamers for recognition cancer markers as well as on the comparison of the properties of nucleic acid aptamers and antibodies. The methods of immobilization of aptamers at the nanoparticles and nano/micromotors are provided. Examples of applications of polymer nanoparticles and nano/micromotors in targeted delivery and in controlled drug release are presented. The future perspectives of biomimetic nanostructures in personalized nanomedicine are also discussed.

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Hybrid Extracellular Vesicles‐Liposomes Camouflaged Magnetic Vesicles Cooperating with Bioorthogonal Click Chemistry for High‐Efficient Melanoma Circulating Tumor Cells Enrichment

Kang

Zhang

Zhou

et al. 2023

Adv Healthcare Materials

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The capture of melanoma circulating tumor cells (melanoma CTCs, MelCTCs) is of great significance for the early diagnosis and personalized treatment of melanoma. The rarity and heterogeneity of MelCTCs have greatly limited the development of MelCTCs capture methods, especially those based on immune/aptamer-affinity. Herein, an extracellular vesicles-camouflaged strategy is designed to functionalize the magnetic nanoparticles (Fe 3 O 4 ) and to generate magnetic vesicles (Fe 3 O 4 @lip/ev) with excellent antifouling and active tumor cell targeting properties. Combined with the bioorthogonal click chemistry, the engineered magnetic vesicles with dibenzocyclooctyne can be widely used to target and separate all the metabolically labeled CTCs with varied phenotypes, organ origin, and even the biological species. The capture efficiency exceeded 80% with an extremely low detection limitation of ten cells. Most importantly, the strategy proposed can be directly applied to enrich MelCTCs from 0.5 mL blood samples of melanoma-bearing mice, with a greatly minimized residue of white blood cells (only 21-568) while ignoring the fluctuations of MelCTC phenotype.

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Improving Tumor Targeting of Exosomal Membrane-Coated Polymeric Nanoparticles by Conjugation with Aptamers

Cited by 54 publications

References 47 publications

<p>Advances in Exosome-Based Drug Delivery and Tumor Targeting: From Tissue Distribution to Intracellular Fate</p>

<p>Advances in Exosome-Based Drug Delivery and Tumor Targeting: From Tissue Distribution to Intracellular Fate</p>

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

Hybrid Extracellular Vesicles‐Liposomes Camouflaged Magnetic Vesicles Cooperating with Bioorthogonal Click Chemistry for High‐Efficient Melanoma Circulating Tumor Cells Enrichment

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