Impurities in Sugar Processing, Determination of Dextran and Starch in Cane Juices and Sugar Products

Nicholson, R. I.; Horsley, Margaret

doi:10.1021/jf60103a011

Cited by 10 publications

(5 citation statements)

References 4 publications

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“…Various methods to detect the content of dextran in sugar and sugar by-product have been developed. (6,7) Antibodydependent Midland Sucro Test TM , (8) which is based on linear increase of turbidity with dextran concentration over a designated time, has limited routine application due to its requirement of specialized equipment and procedure. Alcohol haze method (9) detects the content of dextran based on the haze developed in 50% (v/v) alcohol solution after removal of starch and salt along with precipitation of proteins contained in dextran.…”

mentioning

confidence: 99%

Development of Monoclonal Antibody-Based Sandwich ELISA for Detection of Dextran

Wang

et al. 2014

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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Dextran as anti-nutritional factor is usually a result of bacteria activity and has associated serial problems during the process stream in the sugar industry and in medical therapy. A sensitive method is expected to detect dextran quantitatively. Here we generated four monoclonal antibodies (MAbs) against dextran using dextran T40 conjugated with bovine serum albumin (BSA) as immunogen in our lab following hybridoma protocol. Through pairwise, an MAb named D24 was determined to be conjugated with horseradish peroxidase (HRP) and was used in the establishment of a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) method for determination of dextran, in which MAb D9 was chosen as a capture antibody. The detection limit and working scope of the developed sandwich ELISA method were 3.9 ng/mL and 7.8-500 ng/mL with a correlation coefficient of 0.9909. In addition, the cross-reaction assay demonstrated that the method possessed high specificity with no significant cross-reaction with dextran-related substances, and the recovery rate ranged from 96.35 to 102.00%, with coefficient of variation ranging from 1.58 to 6.94%. These results indicated that we developed a detection system of MAb-based sandwich ELISA to measure dextran and this system should be a potential tool to determine dextran levels.

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confidence: 99%

Development of Monoclonal Antibody-Based Sandwich ELISA for Detection of Dextran

Wang

et al. 2014

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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“…of 95% ethanol and 2 ml. of a saturated potassium chloride solution were added; and the solution was shaken vigorously to aid precipitation (6). After standing overnight, the mixture was centrifuged and the supernatant solution discarded.…”

Section: Methodsmentioning

confidence: 99%

Sugar Crystal Analyses, Determination of Starch in Sucrose Crystal

Tu¹,

Okamoto²

1963

J. Agric. Food Chem.

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“…At present, accumulating methods have been available to measure the content of dextran in sugar and sugar by‐products (Nicholson et al . ; Sarkar et al . ), and among them, alcohol haze method and Roberts’ copper method have been extensively used within sugar industries all over the world up to date (Brown et al .…”

Section: Introductionmentioning

confidence: 96%

Development of A Monoclonal Antibody‐Based Competitive Elisa Method for Quantitative Detection of Dextran

Wang

et al. 2016

Journal of Food Quality

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To solve processing problems caused by bacterial activity in sugar industry, a sensitive method for dextran detection is expected. In this study, monoclonal antibodies (mAbs) against dextran were prepared using dextran T40 conjugated with bovine serum albumin as immunogens. Basing on the mAbs, a competitive enzyme-linked immunosorbent assay (ELISA) method to measure dextran quantitatively was developed. Results demonstrated that this competitive ELISA method had a calibration curve with correlation coefficient of 0.9986, IC 50 value of 204.2 ng/mL and detection value ranging from 26.3 ng/mL to 1174.9 ng/mL. In addition, cross-reaction assay suggested the method had no significant crossreaction with dextran-related substances such as b-glucan, starch, sucrose, sugar and glucose. Besides, spike recovery assay indicated this quantitative method for dextran detection had a perfect accuracy. PRACTICAL APPLICATIONSWith wide detection range, high specificity and good calibration curve, this developed competitive ELISA method will be reliable to detect dextran quantitatively in sugar industry, and even may be commercially applied into kit development.

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Impurities in Sugar Processing, Determination of Dextran and Starch in Cane Juices and Sugar Products

Cited by 10 publications

References 4 publications

Development of Monoclonal Antibody-Based Sandwich ELISA for Detection of Dextran

Development of Monoclonal Antibody-Based Sandwich ELISA for Detection of Dextran

Sugar Crystal Analyses, Determination of Starch in Sucrose Crystal

Development of A Monoclonal Antibody‐Based Competitive Elisa Method for Quantitative Detection of Dextran

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