IMS 1313-nanoparticle Mucosal Vaccine Enhances Immunity Against Avian Influenza and Newcastle Disease Viruses

Hussein, Hussein A.; Ismail, Nermeen M.; El‐Deeb, Ayman H.; Emara, Mohamed M.; Tawfik, Hoda .; Wanis, Nabil Abdel

doi:10.3923/ijps.2018.167.174

Cited by 6 publications

(6 citation statements)

References 32 publications

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“…In light of this, the better performance of the irradiated vaccine during the early phases of the lowest challenge with 10 3 EID 50 suggests the possibility that irradiated antigens administrated via the mucosal route might have reduced the primary attack rate more effectively than formalinated ones. This might depend on the higher avidity/affinity of secretory IgA (S-IgA) mounted against a better preserved antigenic structure inactivated through irradiation (63). A limitation of our study is that we did not assess the IgA levels at the humoral and the mucosal level.…”

Section: Discussionmentioning

confidence: 98%

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Protective Efficacy of H9N2 Avian Influenza Vaccines Inactivated by Ionizing Radiation Methods Administered by the Parenteral or Mucosal Routes

et al. 2022

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H9N2 viruses have become, over the last 20 years, one of the most diffused poultry pathogens and have reached a level of endemicity in several countries. Attempts to control the spread and reduce the circulation of H9N2 have relied mainly on vaccination in endemic countries. However, the high level of adaptation to poultry, testified by low minimum infectious doses, replication to high titers, and high transmissibility, has severely hampered the results of vaccination campaigns. Commercially available vaccines have demonstrated high efficacy in protecting against clinical disease, but variable results have also been observed in reducing the level of replication and viral shedding in domestic poultry species. Antigenic drift and increased chances of zoonotic infections are the results of incomplete protection offered by the currently available vaccines, of which the vast majority are based on formalin-inactivated whole virus antigens. In our work, we evaluated experimental vaccines based on an H9N2 virus, inactivated by irradiation treatment, in reducing viral shedding upon different challenge doses and compared their efficacy with formalin-inactivated vaccines. Moreover, we evaluated mucosal delivery of inactivated antigens as an alternative route to subcutaneous and intramuscular vaccination. The results showed complete protection and prevention of replication in subcutaneously vaccinated Specific Pathogen Free White Leghorn chickens at low-to-intermediate challenge doses but a limited reduction of shedding at a high challenge dose. Mucosally vaccinated chickens showed a more variable response to experimental infection at all tested challenge doses and the main effect of vaccination attained the reduction of infected birds in the early phase of infection. Concerning mucosal vaccination, the irradiated vaccine was the only one affording complete protection from infection at the lowest challenge dose. Vaccine formulations based on H9N2 inactivated by irradiation demonstrated a potential for better performances than vaccines based on the formalin-inactivated antigen in terms of reduction of shedding and prevention of infection.

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Section: Discussionmentioning

confidence: 98%

“…This might depend on the higher avidity/affinity of secretory IgA (S-IgA) mounted against a better preserved antigenic structure inactivated through irradiation ( 63 ). A limitation of our study is that we did not assess the IgA levels at the humoral and the mucosal level.…”

Section: Discussionmentioning

confidence: 99%

Protective Efficacy of H9N2 Avian Influenza Vaccines Inactivated by Ionizing Radiation Methods Administered by the Parenteral or Mucosal Routes

et al. 2022

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“…The acquired humoral response to the second vaccination in TRTs 1, 2, 3, and 7 is most likely due to class switch from IgM to IgG [19]; The data of Table 4, related to acquired CMI to vaccination and to challenge, quantified by the IFN-γ levels in sera of the differently treated birds, document the impact of administering the developed vaccine at two different ages on inducing higher levels of IFN-γ; this response is indispensably needed for protection against v-NDV challenges administered at 28 d of age [25,26]. The data of INF-γ levels post challenge is in agreement with documentations confirming an improvement in INF-γ level of vaccinated -challenged birds compared to unvaccinated -challenged ones [27,28].…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Evaluation of immunity and protection in chicken administered a developed vaccine against predominant Middle Eastern strains of genotypes VI and VII of velogenic Newcastle Disease Virus

2021

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“…The local immune response plays a major part of protection against NDV at the site of entry of pathogens [5]. Many researchers stress the importance of the knowledge on the immunological status of the birds against disease is vital for the proper control of the NDV [6-8]…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mucosal Immunity in Chickens Vaccinated With Oral Pellet Newcastle Disease Vaccine

Kirubaharan¹

2021

Preprint

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The Newcastle disease outbreak in chickens is a continuing threat to the poultry industry. Infection with Newcastle disease is greatly influenced by the immune status of the birds. The mucosal immunity plays a major role in the local immune response in the protection of chickens against diseases. This study was undertaken to find the efficacy of thermostable live oral pellet vaccine in developing the mucosal immunity in chickens. Samples were collected from Harderian gland, Lachrymal fluid, Tracheal fluid, Intestinal washings, Bile and Serum of chickens after administration of oral pellet vaccine to detect presence of NDV specific IgA antibodies. Results showed that there is significant increase in the immune response after one week post vaccination with no significant difference between 14 and 21 days after vaccine. There exists significant difference in Mean OD values between samples of Harderian, Lachrymal, Trachea, Intestine, Bile and serum with bile found to have increased IgA response. Challenge experiment results showed that oral pellet vaccine was able to protect chickens against virus challenge by increasing the mucosal immunity against Newcastle disease.

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IMS 1313-nanoparticle Mucosal Vaccine Enhances Immunity Against Avian Influenza and Newcastle Disease Viruses

Cited by 6 publications

References 32 publications

Protective Efficacy of H9N2 Avian Influenza Vaccines Inactivated by Ionizing Radiation Methods Administered by the Parenteral or Mucosal Routes

Protective Efficacy of H9N2 Avian Influenza Vaccines Inactivated by Ionizing Radiation Methods Administered by the Parenteral or Mucosal Routes

WITHDRAWN: Evaluation of immunity and protection in chicken administered a developed vaccine against predominant Middle Eastern strains of genotypes VI and VII of velogenic Newcastle Disease Virus

Evaluation of Mucosal Immunity in Chickens Vaccinated With Oral Pellet Newcastle Disease Vaccine

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