In a canine model, lung preservation at 10° C is superior to that at 4° C

Long-term (72 hours) preservation of rat lungs

“…The results observed in the no I-R group were those expected for normal lungs. 7,8 Likewise, the results of the LPD24 group were similar to those previously reported, [12][13][14][15][16][17] because gas exchange deteriorated rapidly during the initial 20 to 30 minutes of reperfusion and stabilized thereafter (Figure 1), indicating the presence of I-R injury. Therefore we believe that these observations support the validity, stability, and reproducibility of our experimental model.…”

“…11 Third, in clinical practice there is no consensus about the composition of the ideal preservation solution, the conditions of lung storage, or both. 1,2 We decided to use the LPD24 group (preservation in LPD at 10°C during 24 hours) as a model for the standard preservation regimen because preservation solutions with a low potassium content have been reported to offer good protection of lung function at reperfusion [12][13][14][15][16] and because the optimal temperature of lung storage in LPD has been established at around 10°C. 14 Likewise, we decided to test our hypothesis after a period of 72 hours of ischemia (TEST72 group) because previous experimental studies have already reported good results with LPD after 48 hours of lung preservation.…”

“…1,2 We decided to use the LPD24 group (preservation in LPD at 10°C during 24 hours) as a model for the standard preservation regimen because preservation solutions with a low potassium content have been reported to offer good protection of lung function at reperfusion [12][13][14][15][16] and because the optimal temperature of lung storage in LPD has been established at around 10°C. 14 Likewise, we decided to test our hypothesis after a period of 72 hours of ischemia (TEST72 group) because previous experimental studies have already reported good results with LPD after 48 hours of lung preservation. 15,16 Finally, we decided to use ethanol as an antifreezing agent, but we recognize that many endogenous alcohols (eg, ketona bodies or glycerol), either alone or in combination, might have also been used for this purpose.…”

Lung preservation: from perfusion to temperature

“…Research from the University of Toronto has challenged the standard temperature for static cold storage of 4 o C based on early evidence suggesting that the ideal temperature for lung preservation is 10 o C with lower temperatures having a detrimental effect on mitochondrial function [40]. Using a porcine model, lungs were compared after 36 h of cold static storage at either 4 or 10 o C. The lungs preserved at 10 o C demonstrated significantly better oxygenation and compliance when assessed on an ex-vivo lung perfusion (EVLP) machine.…”

Overview and Future Practice Patterns in Cardiac and Pulmonary Preservation

“…In vivo and in vitro laboratory studies looking for the ideal temperature for lung preservation have determined the ideal preservation temperature to be 1 0°C. [23][24][25] The optimal volume of flush solution is unknown. A single dose of antegrade flushing is standard.…”