In <i>Vitro</i> Effects of the Phytocomplex TrichoTech<sup>TM</sup> on Human Fibroblasts: Proliferative Potential and Effects on Gene Expression of FGF-7 and FGF-10

Abstract

The human hair follicle, a mini-organ formed with neuroectodermal-mesodermal interaction, is a complex structure, in the active steady state (anagen) the dermal papilla can be considered as a ball of extracellular matrix, surrounding specialized fibroblasts. The cross-talk of dermal papilla with neighbouring matrix cells results in the maintenance of hair fibre production. This study aimed to investigate the proliferative potential of the compound Trichotech TM , a phytocomplex obtained from a mixture of essential oils, on cultured human fibroblasts and its ability to modulate the gene expression of FGF-7 and FGF-10. Trichotech TM was shown to enhance fibroblasts proliferation in concentrations of 0.5% to 2.0%, and also increase the percentage of cells in the S/G2/M phases of the cell cycle. Trichotech TM at both 1.0% and 2.0% induced a statistically significant effect on wound healing assay compared to the untreated control. We examined the interaction between cell survival (PI3K/Akt) and mitogenic (Ras/MAPK) signal transduction pathways after Trichotech TM treatment (1.0% and 2.0%) on the fibroblast cell line.

Hair Growth Promoting Effect of Trichoxidil<up>TM</up>: A New Natural Compound for Hair Loss

“…In the present work, hair growth was evaluated by dermoscopy, histological KGF [17], and VEGF [18]. Growth factors that stimulate the anagen phase, comprise fibroblast growth factor 7 (FGF-7 or KGF), hepatocyte growth factor (HGF), IGF-1, prostaglandin E2 (PGE2), and VEGF [19].…”

Compatibility of Different Formulations in TrichoConceptTM Vehicles for Hair Treatments

“…In this study, we explored the compatibility of several active pharmaceutical ingredients (APIs) in bases designed to be used for patients with alopecia, including TrichoWash TM (a readyto-use vehicle for personalized non-irritant shampoos), TrichoCond TM (a ready-to-use vehicle for personalized conditioners and with moisturizing properties), TrichoCream TM (a ready-to-use vehicle for personalized creams for eyelashes, eyebrows, and beards), and TrichoSol TM (a ready-to-use vehicle for personalized hair solutions; consisting of a highly spreadable hydrophilic solution containing mineral salts of vegetable origin and is free from alcohol and propylene glycol), each of which contains TrichoTech TM , a patented technology designed to promote scalp health. All vehicles are free from parabens, formaldehyde, PABA, boric acid, benzyl alcohol, benzyl benzoate, propylene glycol, lanolin, triclosan, 1,4-dioxane, petrolatum, and artificial colorants [4].…”